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. 1982 Aug;79(16):4988–4991. doi: 10.1073/pnas.79.16.4988

Tumor-promoter-resistant cells lack trisialoganglioside response.

L Srinivas, T D Gindhart, N H Colburn
PMCID: PMC346811  PMID: 6956909

Abstract

JB6 mouse epidermal cells shift irreversibly to tumor cell phenotype (anchorage independence and tumorigenicity) on treatment with phorbol esters and other tumor promoters. Exposure to phorbol 12-myristate 13-acetate (PMA) decreased the de novo synthesis of trisialoganglioside (GT) in these "promotable" JB6 cells to 5-10% of that of untreated cells. The GT decrease occurred consistently in promotion-sensitive cells and not in promotion-resistant variants. Insertion of GT into membranes of PMA-treated cells inhibited PMA promotion of transformation as measured by agar colony induction. This ability to inhibit promotion of transformation is specific to GT and is not shared by other sialoglycoconjugates, including gangliosides GM1, GD1a, and asialo-GM1. The mechanism of the blocking activity of GT must be distal to the binding of PMA to its receptors, as exogenously added GT does not inhibit specific binding of tritiated phorbol diester. GT is unable to block agar colony formation by transformed cell lines, showing that its level of action is at induction of the transformed phenotype rather than at expression of it.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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