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. 1982 Aug;79(16):5016–5020. doi: 10.1073/pnas.79.16.5016

Transposon Tn10 provides a promoter for transcription of adjacent sequences.

M S Ciampi, M B Schmid, J R Roth
PMCID: PMC346817  PMID: 6289329

Abstract

Promoters located within the Tn10 insertion element cause transcription of "host" sequences adjacent to both ends of the inserted Tn10 element. These promoters are usually not observed in genetic experiments because their transcripts are efficiently terminated at nearby rho-dependent termination sites. The observations presented here provide an explanation for several confusing aspects of transposon behavior and suggest the possibility that many transposons possess promoters that have escaped detection for similar reasons.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins J. F., Loper J. C. Transcription initiation in the histidine operon of Salmonella typhimurium. Proc Natl Acad Sci U S A. 1970 Apr;65(4):925–932. doi: 10.1073/pnas.65.4.925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg D. E., Weiss A., Crossland L. Polarity of Tn5 insertion mutations in Escherichia coli. J Bacteriol. 1980 May;142(2):439–446. doi: 10.1128/jb.142.2.439-446.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blazey D. L., Burns R. O. Transcriptional activity of the transposable element Tn10 in the Salmonella typhimurium ilvGEDA operon. Proc Natl Acad Sci U S A. 1982 Aug;79(16):5011–5015. doi: 10.1073/pnas.79.16.5011. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brewster J. M., Morgan E. A. Tn9 and IS1 inserts in a ribosomal ribonucleic acid operon of Escherichia coli are incompletely polar. J Bacteriol. 1981 Dec;148(3):897–903. doi: 10.1128/jb.148.3.897-903.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chumley F. G., Roth J. R. Genetic fusions that place the lactose genes under histidine operon control. J Mol Biol. 1981 Feb 5;145(4):697–712. doi: 10.1016/0022-2836(81)90310-7. [DOI] [PubMed] [Google Scholar]
  6. Ely B., Ciesla Z. Internal promoter P2 of the histidine operon of Salmonella typhimurium. J Bacteriol. 1974 Nov;120(2):984–986. doi: 10.1128/jb.120.2.984-986.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Errede B., Cardillo T. S., Sherman F., Dubois E., Deschamps J., Wiame J. M. Mating signals control expression of mutations resulting from insertion of a transposable repetitive element adjacent to diverse yeast genes. Cell. 1980 Nov;22(2 Pt 2):427–436. doi: 10.1016/0092-8674(80)90353-0. [DOI] [PubMed] [Google Scholar]
  8. Foster T. J. Insertion of the tetracycline resistance translocation unit Tn10 in the lac operon of Escherichia coli K12. Mol Gen Genet. 1977 Sep 9;154(3):305–309. doi: 10.1007/BF00571287. [DOI] [PubMed] [Google Scholar]
  9. Housley P. R., Leavitt A. D., Whitfield H. J. Genetic analysis of a temperature-sensitive Salmonella typhimurium rho mutant with an altered rho-associated polycytidylate-dependent adenosine triphosphatase activity. J Bacteriol. 1981 Jul;147(1):13–24. doi: 10.1128/jb.147.1.13-24.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Johnston H. M., Roth J. R. Genetic analysis of the histidine operon control region of Salmonella typhimurium. J Mol Biol. 1981 Feb 5;145(4):713–734. doi: 10.1016/0022-2836(81)90311-9. [DOI] [PubMed] [Google Scholar]
  11. Kleckner N., Chan R. K., Tye B. K., Botstein D. Mutagenesis by insertion of a drug-resistance element carrying an inverted repetition. J Mol Biol. 1975 Oct 5;97(4):561–575. doi: 10.1016/s0022-2836(75)80059-3. [DOI] [PubMed] [Google Scholar]
  12. Kleckner N., Reichardt K., Botstein D. Inversions and deletions of the Salmonella chromosome generated by the translocatable tetracycline resistance element Tn10. J Mol Biol. 1979 Jan 5;127(1):89–115. doi: 10.1016/0022-2836(79)90461-3. [DOI] [PubMed] [Google Scholar]
  13. Kleckner N., Roth J., Botstein D. Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977 Oct 15;116(1):125–159. doi: 10.1016/0022-2836(77)90123-1. [DOI] [PubMed] [Google Scholar]
  14. Kleckner N., Steele D. A., Reichardt K., Botstein D. Specificity of insertion by the translocatable tetracycline-resistance element Tn10. Genetics. 1979 Aug;92(4):1023–1040. doi: 10.1093/genetics/92.4.1023. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. MCCLINTOCK B. Controlling elements and the gene. Cold Spring Harb Symp Quant Biol. 1956;21:197–216. doi: 10.1101/sqb.1956.021.01.017. [DOI] [PubMed] [Google Scholar]
  16. Martin R. G., Silbert D. F., Smith W. E., Whitfield H. J., Jr Polarity in the histidine operon. J Mol Biol. 1966 Nov 14;21(2):357–369. doi: 10.1016/0022-2836(66)90104-5. [DOI] [PubMed] [Google Scholar]
  17. Morgan E. A. Insertions of Tn 10 into an E. coli ribosomal RNA operon are incompletely polar. Cell. 1980 Aug;21(1):257–265. doi: 10.1016/0092-8674(80)90133-6. [DOI] [PubMed] [Google Scholar]
  18. Reynolds A. E., Felton J., Wright A. Insertion of DNA activates the cryptic bgl operon in E. coli K12. Nature. 1981 Oct 22;293(5834):625–629. doi: 10.1038/293625a0. [DOI] [PubMed] [Google Scholar]
  19. Roeder G. S., Fink G. R. DNA rearrangements associated with a transposable element in yeast. Cell. 1980 Aug;21(1):239–249. doi: 10.1016/0092-8674(80)90131-2. [DOI] [PubMed] [Google Scholar]
  20. Schmidt F. J., Jorgensen R. A., de Wilde M., Davies J. E. A specific tetracycline-induced, low-molecular-weight RNA encoded by the inverted repeat of Tn10 (IS10). Plasmid. 1981 Jul;6(1):148–150. doi: 10.1016/0147-619x(81)90061-5. [DOI] [PubMed] [Google Scholar]
  21. Williamson V. M., Young E. T., Ciriacy M. Transposable elements associated with constitutive expression of yeast alcohol dehydrogenase II. Cell. 1981 Feb;23(2):605–614. doi: 10.1016/0092-8674(81)90156-2. [DOI] [PubMed] [Google Scholar]

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