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. 1998 Jan;15(1):50–53. doi: 10.1023/A:1022582422932

Effectiveness of Menuzo's B2 Medium with Buffalo Rat Liver Cells for Development of In Vitro Matured/In Vitro Fertilized Bovine Oocytes

R L Krisher 1, J R Gibbons 1, F C Gwazdauskas 1,✉
PMCID: PMC3468206  PMID: 9493067

Abstract

Purpose:The effectiveness of two culture media on the development of bovine embryos in a buffalo rat liver (BRL) coculture system was investigated.

Methods:Bovine oocytes were matured and fertilized in vitro, then cocultured, 25 per well, for 7 days in 500 μl of modified M199 or modified Menuzo's B2 medium over a BRL cell monolayer at 39°C in an atmosphere of 5% CO2in air. Medium 199 was modified by the addition of 10% of (v/v) fetal bovine serum (FBS), 9 mg/ml bovine serum albumin, 2 mM glycine, 1 mM alanine, and 0.1 mM nonessential amino acids (NEAA). Menuzo's B2 medium was modified by the addition of 10% (v/v) FBS, 1 mM alanine, and 0.1 mM NEAA.

Results:Modified Menuzo's B2 medium improved embryo development to the morula or blastocyst stage compared to modified M199 (121/353, 34.3%, versus 99/362, 27.3%, P<0.05).

Conclusions:These results suggest that Menuzo's B2 medium with modifications in a BRL coculture system can provide a significant benefit for culture of early bovine embryos over the traditional use of medium 199.

Keywords: bovine, buffalo rat liver cells, embryo culture

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