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. 2002 Feb;19(2):60–66. doi: 10.1023/A:1014439529813

The Mitochondrial DNA Mutation (ΔmtDNA5286) in Human Oocytes: Correlation with Age and IVF Outcome

Vered Yesodi 1, Yuval Yaron 2, Joseph B Lessing 1, Ami Amit 1, Dalit Ben-Yosef 1,✉
PMCID: PMC3468229  PMID: 11958506

Abstract

Purpose: To evaluate the correlation between the presence of a newly described mitochondrial DNA (mtDNA) mutation (ΔmtDNA5286), patients' age, and in vitro fertilization (IVF) outcomes.

Methods: The presence of ΔmtDNA5286 was analyzed by nested-primer PCR in 224 unfertilized oocytes of 81 women undergoing IVF for various reasons. Age, number of oocytes retrieved, fertilization and embryo cleavage, number of embryos transferred, and pregnancy rates were compared between patients with and without a mtDNA mutation in their oocytes.

Results: The 23 patients in which ΔmtDNA5286 was detected in at least one oocyte were significantly younger than the other 58 with no mutations (30.9 years vs. 33.8 years, respectively, P = 0.03), and had a significantly lower fertilization rate (28.9% vs. 42.1%, respectively, P = 0.01). No other outcome variable was significantly different between the two groups.

Conclusion(s): The mtDNA mutation ΔmtDNA5286 may serve as a marker of decreased oocyte quality in IVF.

Keywords: Fertilization, human oocytes, mitochondrial DNA, mutations

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