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Journal of Assisted Reproduction and Genetics logoLink to Journal of Assisted Reproduction and Genetics
. 2002 Mar;19(3):137–143. doi: 10.1023/A:1014788821965

Protein Supplementation of Human IVF Culture Media

Deborah Blake 1, Peter Svalander 2, Meishan Jin 2, Christer Silversand 2, Lars Hamberger 2,
PMCID: PMC3468252  PMID: 12005309

Abstract

This review travels the road of protein supplementation in embryo culture development—from whole crude plasma in the mid Twentieth century moving through to the completely genetically engineered human albumin with successful births at the beginning of the Twenty-first.

Keywords: Albumin, culture media, human IVF, recombinant albumin, serum

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REFERENCES

  • 1.Steptoe PC, Edwards RG. Re-implantation of a human embryo with subsequent tubal pregnancy. Lancet. 1976;1:880–882. doi: 10.1016/s0140-6736(76)92096-1. [DOI] [PubMed] [Google Scholar]
  • 2.Nygren KG, Nyboe Andersen A. Assisted reproductive technology in Europe, 1997. Results generated from European registers by ESHRE. Hum Reprod. 2001;16:384–391. doi: 10.1093/humrep/16.2.384. [DOI] [PubMed] [Google Scholar]
  • 3.Bavister BD. Culture of preimplantation embryos: Facts and artifacts. Hum Reprod Update. 1995;1:91–148. doi: 10.1093/humupd/1.2.91. [DOI] [PubMed] [Google Scholar]
  • 4.Hammond J. Recovery and culture of tubal mouse ova. Nature. 1949;163:28. doi: 10.1038/163028b0. [DOI] [PubMed] [Google Scholar]
  • 5.Whittingham DG. Culture of mouse ova. J Reprod Fert Suppl. 1971;14:7–21. [PubMed] [Google Scholar]
  • 6.Whittingham DG. Culture of mouse ova. J Reprod Fert Suppl. 1971;14:7–21. [PubMed] [Google Scholar]
  • 7.Whitten WK. Culture of tubal mouse ova. Nature. 1956;177:96. doi: 10.1038/177096a0. [DOI] [PubMed] [Google Scholar]
  • 8.Biggers JK, Brinster RL. Biometrical problems in the study of early mammalian embryos in vitro. J Exp Zool. 1965;158:39. doi: 10.1002/jez.1401580104. [DOI] [PubMed] [Google Scholar]
  • 9.Whittingham DG. Fertilisation of mouse eggs in vitro. Nature. 1968;220:592. doi: 10.1038/220592a0. [DOI] [PubMed] [Google Scholar]
  • 10.Leese HJ. Formation and function of oviduct fluid. J Reprod Fertil. 1988;82:843–856. doi: 10.1530/jrf.0.0820843. [DOI] [PubMed] [Google Scholar]
  • 11.Mehta TS, Kiessling AA. Development potential of mouse embryos conceived in vitro and cultured in ethylenediaminetetraacetic acid with or without amino acids or serum. Biol Reprod. 1990;43:600–606. doi: 10.1095/biolreprod43.4.600. [DOI] [PubMed] [Google Scholar]
  • 12.Caro CM, Trounson A. The effect of protein on preimplantation mouse embryo development in vitro. J In Vitro Fert Embryo Transf. 1984;1:183–187. doi: 10.1007/BF01139212. [DOI] [PubMed] [Google Scholar]
  • 13.Ali J, Shahata MA, Al-Natsha SD. Formulation of a proteinfree medium for human assisted reproduction. Hum Reprod. 2000;15:145–156. doi: 10.1093/humrep/15.1.145. [DOI] [PubMed] [Google Scholar]
  • 14.Kaye PL, Gardner HG. Preimplantation access to maternal insulin and albumin increases fetal growth rate in mice. Hum Reprod. 1999;14:3052–3059. doi: 10.1093/humrep/14.12.3052. [DOI] [PubMed] [Google Scholar]
  • 15.Alvarez JG, Storey BT. Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol Reprod Dev. 1995;42:334–346. doi: 10.1002/mrd.1080420311. [DOI] [PubMed] [Google Scholar]
  • 16.Claassens OE, Harrison KL: Optimising sensitivity of the human sperm motility assay for embryology toxicity testing. In Proceedings of the World Congress on IVF and Human Reproductive Genetics 1999.
  • 17.Fissore RA, Jackson KV, Kiessling AA. Mouse zygote development in culture medium without protein in the presence of ethylenediaminetetraacetic acid. Biol Reprod. 1989;41:835–841. doi: 10.1095/biolreprod41.5.835. [DOI] [PubMed] [Google Scholar]
  • 18.Pemble LB, Kaye PL. Whole protein uptake and metabolism by mouse blastocysts. J Reprod Fertil. 1976;78:149–157. doi: 10.1530/jrf.0.0780149. [DOI] [PubMed] [Google Scholar]
  • 19.Brown JR, Shockley P. Serum albumin: Structure and characterisation of its ligand binding sites. In: Jost PC, Griffith OH, editors. Lipid-Protein Interactions. New York: Wiley; 1982. pp. 22–68. [Google Scholar]
  • 20.Ammit AJ, O'Neill CO. The role of albumin in the release of platelet-activating factor by mouse pre-implantation embryos in vitro. J Reprod Fert. 1997;109:309–318. doi: 10.1530/jrf.0.1090309. [DOI] [PubMed] [Google Scholar]
  • 21.Leung PCS, Gronow MJ, Kellow GN, Lopata A, Speirs AL, McBain JC, du Plessis YP, Johnston I. Serum supplement in human in vitro fertilization and embryo development. Fertil Steril. 1984;41:36–39. doi: 10.1016/s0015-0282(16)47537-0. [DOI] [PubMed] [Google Scholar]
  • 22.Law SKA, Reid KBM. Complement. Oxford: IRL Press; 1988. pp. 9–27. [Google Scholar]
  • 23.Imoedemhe DA, Sigue AB, Pacpaco EL, Olazo AB, Luciano EC. Absence of heat treatment of serum for culture medium supplementation does not adversely affect the outcome of in-vitro fertilization. Hum Reprod. 1994;9:1749–1751. doi: 10.1093/oxfordjournals.humrep.a138787. [DOI] [PubMed] [Google Scholar]
  • 24.Kemer P, Feichtinger W. Pregnancy following in vitro fertilization and embryo transfer using pure human serum as culture and transfer medium. Fertil Steril. 1984;6:936–937. doi: 10.1016/s0015-0282(16)47912-4. [DOI] [PubMed] [Google Scholar]
  • 25.Alberda AT, VanOs HC, Zeilmaker GH. Hepatitis Bvirusinfectie bij Vrouwen behandeld met in-vitro fertilisatie. Nederlands Tijdschrift Voor Gieneeskunde. 1989;133:20–25. [PubMed] [Google Scholar]
  • 26.Shaw JM, Harrison KL, Wilson LM, Breen TM, Shaw G, Cummins JM, Hennessey JF. Results using medium supplemented with either fresh or frozen stored serum in human in vitro fertilization. J In Vitro Fert Embryo Transf. 1987;4:5–9. doi: 10.1007/BF01555427. [DOI] [PubMed] [Google Scholar]
  • 27.Leveille MC, Carnegie J, Tanphaichitr N. Effects of human sera and human serum albumin on mouse embryo culture. J Assist Reprod Genet. 1992;9:45–52. doi: 10.1007/BF01204114. [DOI] [PubMed] [Google Scholar]
  • 28.Dokras A, Sargen IL, Redman CWG. Sera from women with unexplained infertility inhibit both mouse and human embryo growth in vitro. Fertil Steril. 1993;60:285–292. doi: 10.1016/s0015-0282(16)56099-3. [DOI] [PubMed] [Google Scholar]
  • 29.Thompson JG, Gardner DK, Pugh A, McMillan WH, Tervit HR. Lamb birth weight is affected by culture system utilized during in vitro pre-elongation development of ovine embryos. Biol Reprod. 1995;53:1385–1391. doi: 10.1095/biolreprod53.6.1385. [DOI] [PubMed] [Google Scholar]
  • 30.Gardner DK, Lane M, Spitzer A, Batt P. Enhanced rates of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: Amino acids, vitamins, and culturing embryos in groups stimulate development. Biol Repord. 1994;50:390–400. doi: 10.1095/biolreprod50.2.390. [DOI] [PubMed] [Google Scholar]
  • 31.Leese HJ, Donnay I, Thompson JG. Human assisted conception: A cautionary tale. Lessons from domestic animals. Hum Reprod. 1998;13(Suppl4):184–202. doi: 10.1093/humrep/13.suppl_4.184. [DOI] [PubMed] [Google Scholar]
  • 32.Ashwood-Smith MJ, Hollands P, Edwards RG. The use of Albuminar (TM) as a medium supplement in clinical IVF. Hum Reprod. 1989;4:702–705. doi: 10.1093/oxfordjournals.humrep.a136970. [DOI] [PubMed] [Google Scholar]
  • 33.Pool TB, Martin JE. High continuing pregnancy rates following in vitro fertilization—embryo transfer using medium supplemented with a plasma protein fraction containing alpha and beta globulins. Fertil Steril. 1994;61:714–719. doi: 10.1016/s0015-0282(16)56651-5. [DOI] [PubMed] [Google Scholar]
  • 34.Alder A, McVicker-Reing A, Bedford MJ, Alikani M, Cohen J. Plasmanate as a medium supplement for in vitro fertilization. J Assist Repord Genet. 1993;10:67–71. doi: 10.1007/BF01204443. [DOI] [PubMed] [Google Scholar]
  • 35.Weathersbee PS, Pool TB, Ord T. Synthetic serum substitute (SSS): A globulin-enriched protein supplement for human embryo culture. J Assist Reprod Genet. 1995;12:354–360. doi: 10.1007/BF02215726. [DOI] [PubMed] [Google Scholar]
  • 36.Menezo Y: Culture media and embryo metabolism: The influence of serum addition. In In Vitro Fertilization and Embryo Transfer,WF eichtinger (ed), 1984, p 159
  • 37.Batt PA, Gardner DK, Cameron AWN. Oxygen concentration and protein source affect the development of pre-implantation goat embryos in vitro. Repord Fertil Dev. 1991;3:601–607. doi: 10.1071/rd9910601. [DOI] [PubMed] [Google Scholar]
  • 39.Khan I, Staessen C, Devroey P, Van Steirteghem AC. Human serum albumin versus serum: A comparative study on embryo transfer medium. Fertil Steril. 1991;56:98–101. [PubMed] [Google Scholar]
  • 40.Hargreaves CA, Rahman F, Cowan D, Santis M, Keefe T, Howell RJ, Chard T, Homa ST. Serum is more effective than albumin in promoting human embryo development and implantation. Fertil Steril. 1995;64:1162–1166. doi: 10.1016/s0015-0282(16)57978-3. [DOI] [PubMed] [Google Scholar]
  • 41.Staessen C, Van den Abbeel E, Carle M, Khan I, Devroey P, Van Steirteghem AC. Comparison between human serum and Albuminar-20 (TM) supplement for in-vitro fertilisation. Hum Reprod. 1990;5:336–341. doi: 10.1093/oxfordjournals.humrep.a137100. [DOI] [PubMed] [Google Scholar]
  • 42.Hink JH, Hidalgo J, Seeberg VP, Johnson FF. Preparation and properties of a heat-treated human plasma protein in fraction. Vox Sang. 1957;2:174–186. doi: 10.1111/j.1423-0410.1957.tb03452.x. [DOI] [PubMed] [Google Scholar]
  • 43.Wells JA, Wittek AE, Epstein JS. Inactivation and partition of human T-cell lymphotropic virus, type III, during ethanol fractionation of plasma. Transfusion. 1986;26:210–213. doi: 10.1046/j.1537-2995.1986.26286152919.x. [DOI] [PubMed] [Google Scholar]
  • 44.Klein R, Dumble LJ. Transmission of Creutzfeldt-Jakob disease by blood transfusion. Lancet. 1993;341:768. doi: 10.1016/0140-6736(93)90549-v. [DOI] [PubMed] [Google Scholar]
  • 45.Otani T. Earthquakes and prions [letter] Fertil Steril. 1995;63:1137–1139. doi: 10.1016/s0015-0282(16)57568-2. [DOI] [PubMed] [Google Scholar]
  • 46.Svalander P, Jin Z, Larsson A, Hamberger L, Jin M: Pre-clinical studies in the mouse with sequential culture media, r-S1 and r-S2, containing recombinant human albumin. ESHRE Proc 2000;O184 oral presentation
  • 47.Gray CW, Morgan PM, Kane MT. Purification of an embryotrophic factor from commercial bovine serum albumin and its identification as citrate. J Reprod Fertil. 1992;94:471–480. doi: 10.1530/jrf.0.0940471. [DOI] [PubMed] [Google Scholar]
  • 48.Mosley AK, Brouwer KL. Heat treatment of human serum to inactivate HIV does not alter protein binding of selected drugs. Ther Drug Monit. 1997;19:477–479. doi: 10.1097/00007691-199708000-00019. [DOI] [PubMed] [Google Scholar]
  • 49.Gardner DK, Lane M: Recombinant human serum albumin and hyaluronan can replace blood-derived albumin in embryo culture media. Fertil Steril 74Suppl 1:0–086
  • 50.Bungum M, Humaidan P, Bungum L: Successful pregnancies after culturing embryos in sequential media containing recombinant human albumin. Reproductive Biomedicine On Line Submitted.

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