Abstract
Purpose: To analyze the distribution of α tubulins and acetylated α tubulins and the chromatin configuration in abnormally fertilized zygotes from a patient with a multifollicular ovarian response after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI).
Methods: Immunofluorescence and phase contrast microscopy was performed in abnormally fertilized zygotes.
Results: After phase contrast microscopy analysis, immunofluorescence staining was performed in 20 oocytes that developed ≥3 pronuclei (PN) and karyomeres after IVF–ICSI. Around 80% of the abnormal zygotes from IVF were the consequence of monospermic fertilizations. Retention of the second polar body (PB) and the presumptive split of ≥1 PN within the cytoplasm were the main events present in most oocytes after IVF–ICSI.
Conclusions: Fluorescence labeling of selected sperm and oocyte components affords a unique view of abnormal fertilized zygotes. Surprisingly, anomalies detected after IVF–ICSI showed similar etiologies in this special group of zygotes.
Keywords: Abnormal fertilization, immunofluorescence, multifollicular response, poor quality oocytes
Full Text
The Full Text of this article is available as a PDF (1.0 MB).
REFERENCES
- 1.Nagy ZP, Liu J, Joris H, Devroey P, Van Steirteghem A. Timecourse of oocyte activation, pronucleus formation and cleavage in human oocytes fertilized by intracytoplasmic sperm injection. Hum Reprod. 1995;9:1743–1748. doi: 10.1093/oxfordjournals.humrep.a138786. [DOI] [PubMed] [Google Scholar]
- 2.Dor J, Schulman A, Levran D, Ben-Rafael Z, Rudak E, Mashiach S. The treatment of patients with Polycystic Ovarian Syndrome by in vitro fertilization and embryo transfer. A comparison of results with those of patients with tubal infertility. Hum Reprod. 1990;5:816–818. doi: 10.1093/oxfordjournals.humrep.a137189. [DOI] [PubMed] [Google Scholar]
- 3.Homburg R, Berkovitz D, Levy T, Felberg D, Ashkenazi J, Ben-Rafael Z. In vitro fertilization and embryo transfer of infertility associated with polycystic ovary for the treatment of infertility associated with polycystic ovary syndrome. Fertil Steril. 1993;60:858–863. doi: 10.1016/s0015-0282(16)56287-6. [DOI] [PubMed] [Google Scholar]
- 4.Ashkenazi J, Farhi J, Orvieto R, Homburg R, Dekel A, Feldberg D, Ben Rafael Z. Polycystic ovary syndrome patients as oocyte donors: The effect of ovarian stimulation protocol on the implantation rate of the recipient. Fertil Steril. 1995;64:564–567. doi: 10.1016/s0015-0282(16)57793-0. [DOI] [PubMed] [Google Scholar]
- 5.Erickson GF, Yen SSC. New data on follicle cells in polycistic ovaries:Aproposed mechanism for the genesis of cystic follicles. Semin Reprod Endocrinol. 1984;42:39–43. [Google Scholar]
- 6.Rawe VY, Brugo Olmedo S, Nodar FN, Doncel GF, Acosta AA, Vitullo AD. Cytoskeletal organisation defects and abortive activation in human oocytes after IVF and ICSI failure. Mol Hum Reprod. 2000;6:510–516. doi: 10.1093/molehr/6.6.510. [DOI] [PubMed] [Google Scholar]