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. 2012 Aug 13;9:68. doi: 10.1186/1742-4690-9-68

Figure 6.

Figure 6

Kinetics of the ATP-dependent excision of AZTMP and d4TMP from DNA/DNA template-primers. Time course experiments for the excision reaction of AZTMP- and d4TMP-terminated primers (26-mers) annealed to their corresponding 38-nucleotide DNA templates (30 nM) were determined in the presence of 3.2 mM ATP. The excision reaction was catalyzed by WT and mutant RTs (210 nM). The calculated kobs values for the AZTMP excision reaction were 2.82 x 10-3 ± 1.48 x10-4 min-1 for WT RT, 2.42 x 10-3 ± 1.22 x 10-4 min-1 for mutant R284K RT, 4.69 x 10-2 ± 1.69 x 10-3 min-1 for M41L/L210W/T215Y RT, and 3.64 x10-2 ± 3.08 x 10-3 min-1 for M41L/L210W/T215Y/R284K RT. For the excision of d4TMP, the kobs values for WT and mutants R284K, M41L/L210W/T215Y, and M41L/L210W/T215Y/R284K were 3.37 x 10-3 ± 1.93 x 10-4 min-1, 2.62 x 10-3 ± 1.42 x 10-4 min-1, 3.72 x 10-2 ± 1.58 x 10-3 min-1, and 3.70 x 10-2 ± 2.29 x 10-3 min-1, respectively.