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. 2012 Aug 22;9:69. doi: 10.1186/1742-4690-9-69

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Copyright ©2012 Negri et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transduction of murine DC with lentiviral vectors expressing GFP. (a) Murine bone marrow-derived dendritic cells (BMDC) differentiated with GM-CSF (GM-DC) were transduced with normalized amounts of IDLV-GFP, IDLV-GFP/Vpx or LV-GFP (MOI 1) or left untreated (no vector). After 5 days from transduction cells were analysed by FACS to evaluate the percentage of GFP expressing cells. A representative experiment out of three performed is shown. The percentages of GFP-expressing CD11c + DC are indicated within the dot plots. (b) Murine BMDC differentiated in the presence of GM-CSF alone (GM-DC), GM-CSF and IL-4 (GM+IL4-DC) or Flt3L alone (FL-DC) were transduced with normalized amounts of IDLV-GFP, IDLVV-GFP/Vpx (MOI 4) or left untreated (no vector). The expression of GFP was analyzed on CD11c + population. Percentages of GFP expressing cells are indicated within the histograms. A representative experiment out of three performed is shown.