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. 1982 Sep;79(17):5347–5351. doi: 10.1073/pnas.79.17.5347

Incompatibility of plasmids containing the replication origin of the Escherichia coli chromosome.

K Yamaguchi, M Yamaguchi, J Tomizawa
PMCID: PMC346893  PMID: 6291040

Abstract

Plasmids containing the replication origin of the Escherichia coli chromosome (oriC plasmids) are unstable in certain recA strains of E. coli. However, they can be maintained more stably in other recA strains. This stable maintenance has allowed us to study the incompatibility properties of oriC plasmids. We have found that two oriC plasmids are incompatible: they cannot be stably coinherited in individual dividing cells. An oriC plasmid is excluded from growing bacteria at a much faster rate in the presence of a hybrid plasmid made from an oriC plasmid and a high-copy-number vector plasmid than in the presence of another oriC plasmid. By inserting various segments around the oriC region into high-copy-number vectors, we have shown that two different regions in the vicinity of the oriC region determine incompatibility. One region, which we named incA, includes the region essential for autonomous replication of the oriC plasmid. The other, incB, is adjacent to incA but is not required for autonomous replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Crosa J. H., Falkow S. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977;2(2):95–113. [PubMed] [Google Scholar]
  2. Cooper S., Helmstetter C. E. Chromosome replication and the division cycle of Escherichia coli B/r. J Mol Biol. 1968 Feb 14;31(3):519–540. doi: 10.1016/0022-2836(68)90425-7. [DOI] [PubMed] [Google Scholar]
  3. Crosa J. H., Luttropp L. K., Falkow S. Nature of R-factor replication in the presence of chloramphenicol. Proc Natl Acad Sci U S A. 1975 Feb;72(2):654–658. doi: 10.1073/pnas.72.2.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirota Y., Yasuda S., Yamada M., Nishimura A., Sugimoto K., Sugisaki H., Oka A., Takanami M. Structural and functional properties of the Escherichia coli origin of DNA replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):129–138. doi: 10.1101/sqb.1979.043.01.019. [DOI] [PubMed] [Google Scholar]
  5. Iida S., Meyer J., Arber W. The insertion element IS1 is a natural constituent of coliphage P1 DNA. Plasmid. 1978 Jun;1(3):357–365. doi: 10.1016/0147-619x(78)90051-3. [DOI] [PubMed] [Google Scholar]
  6. Itoh T., Tomizawa J. Formation of an RNA primer for initiation of replication of ColE1 DNA by ribonuclease H. Proc Natl Acad Sci U S A. 1980 May;77(5):2450–2454. doi: 10.1073/pnas.77.5.2450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Itoh T., Tomizawa J. Initiation of replication of plasmid ColE1 DNA by RNA polymerase, ribonuclease H, and DNA polymerase I. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):409–417. doi: 10.1101/sqb.1979.043.01.047. [DOI] [PubMed] [Google Scholar]
  8. Jacq A., Kohiyama M. A DNA-binding protein specific for the early replicated region of the chromosome obtained from Escherichia coli membrane fractions. Eur J Biochem. 1980 Mar;105(1):25–31. doi: 10.1111/j.1432-1033.1980.tb04470.x. [DOI] [PubMed] [Google Scholar]
  9. Kuempel P. L., Maglothin P., Prescott D. M. Bidirectional termination of chromosome replication in Escherichia coli. Mol Gen Genet. 1973 Sep 5;125(1):1–8. doi: 10.1007/BF00292981. [DOI] [PubMed] [Google Scholar]
  10. Lother H., Messer W. Promoters in the E. coli replication origin. Nature. 1981 Nov 26;294(5839):376–378. doi: 10.1038/294376a0. [DOI] [PubMed] [Google Scholar]
  11. Louarn J., Funderburgh M., Bird R. E. More precise mapping of the replication origin in Escherichia coli K-12. J Bacteriol. 1974 Oct;120(1):1–5. doi: 10.1128/jb.120.1.1-5.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Low B. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A. 1968 May;60(1):160–167. doi: 10.1073/pnas.60.1.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marsh R. C., Worcel A. A DNA fragment containing the origin of replication of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2720–2724. doi: 10.1073/pnas.74.7.2720. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Messer W., Meijer M., Bergmans H. E., Hansen F. G., von Meyenburg K., Beck E., Schaller H. Origin of replication, oriC, of the Escherichia coli K12 chromosome: nucleotide sequence. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):139–145. doi: 10.1101/sqb.1979.043.01.020. [DOI] [PubMed] [Google Scholar]
  15. Monk M., Kinross J. Conditional lethality of recA and recB derivatives of a strain of Escherichia coli K-12 with a temperature-sensitive deoxyribonucleic acid polymerase I. J Bacteriol. 1972 Mar;109(3):971–978. doi: 10.1128/jb.109.3.971-978.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Novick R. P., Clowes R. C., Cohen S. N., Curtiss R., 3rd, Datta N., Falkow S. Uniform nomenclature for bacterial plasmids: a proposal. Bacteriol Rev. 1976 Mar;40(1):168–189. doi: 10.1128/br.40.1.168-189.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ogura T., Miki T., Hiraga S. Copy-number mutants of the plasmid carrying the replication origin of the Escherichia coli chromosome: evidence for a control region of replication. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3993–3997. doi: 10.1073/pnas.77.7.3993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Oka A., Sugimoto K., Takanami M., Hirota Y. Replication origin of the Escherichia coli K-12 chromosome: the size and structure of the minimum DNA segment carrying the information for autonomous replication. Mol Gen Genet. 1980 Apr;178(1):9–20. doi: 10.1007/BF00267207. [DOI] [PubMed] [Google Scholar]
  19. Shimada K., Ogawa H., Tomizawa J. Studies on radiation-sensitive mutants of E. coli. II. Breakage and repair of ultraviolet irradiated intracellular DNA of phage lambda. Mol Gen Genet. 1968 May 3;101(3):245–256. doi: 10.1007/BF00271626. [DOI] [PubMed] [Google Scholar]
  20. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  21. Sutcliffe J. G. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. Nucleic Acids Res. 1978 Aug;5(8):2721–2728. doi: 10.1093/nar/5.8.2721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tomizawa J., Itoh T. Plasmid ColE1 incompatibility determined by interaction of RNA I with primer transcript. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6096–6100. doi: 10.1073/pnas.78.10.6096. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tomizawa J., Itoh T., Selzer G., Som T. Inhibition of ColE1 RNA primer formation by a plasmid-specified small RNA. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1421–1425. doi: 10.1073/pnas.78.3.1421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Yamaguchi K., Tomizawa J. Establishment of Escherichia coli cells with an integrated high copy number plasmid. Mol Gen Genet. 1980;178(3):525–533. doi: 10.1007/BF00337857. [DOI] [PubMed] [Google Scholar]
  25. Yasuda S., Hirota Y. Cloning and mapping of the replication origin of Escherichia coli. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5458–5462. doi: 10.1073/pnas.74.12.5458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. von Meyenburg K., Hansen F. G., Nielsin L. D., Riise E. Origin of replication, oriC, or the Escherichia coli chromosome on specialized transducing phages lambda asn. Mol Gen Genet. 1978 Apr 17;160(3):287–295. doi: 10.1007/BF00332972. [DOI] [PubMed] [Google Scholar]
  27. von Meyenburg K., Hansen F. G., Riise E., Bergmans H. E., Meijer M., Messer W. Origin of replication, oriC, of the Escherichia coli K12 chromosome: genetic mapping and minichromosome replication. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):121–128. doi: 10.1101/sqb.1979.043.01.018. [DOI] [PubMed] [Google Scholar]

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