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. 1982 Sep;79(17):5357–5361. doi: 10.1073/pnas.79.17.5357

Evidence for DNA modification in the maintenance of X-chromosome inactivation of adult mouse tissues.

V M Chapman, P G Kratzer, L D Siracusa, B A Quarantillo, R Evans, R M Liskay
PMCID: PMC346895  PMID: 6957868

Abstract

The role of DNA modification in the maintenance of mammalian X-chromosome inactivation was investigated by using the technique of DNA transformation in mammalian cells. The ability of inactive X-chromosome DNA from adult mouse tissues to act in transformation for the X-linked hypoxanthine phosphoribosyltransferase gene (Hprt) could be ascertained by utilizing a recently discovered electrophoretic variant form of the hypoxanthine phosphoribosyltransferase enzyme and a previously available X:autosome translocation. Our findings indicate that inactive X-chromosome DNA from several tissues of adult female mice is strikingly inefficient, in comparison to active X-chromosome DNA, in eliciting genetic transformation for hypoxanthine phosphoribosyltransferase. These results provide in vivo evidence that is consistent with DNA modification playing an important role in the maintenance of X-chromosome inactivation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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