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. 1982 Sep;79(17):5435–5437. doi: 10.1073/pnas.79.17.5435

Isolation and amino acid sequence analysis of a 4,000-dalton dynorphin from porcine pituitary.

W Fischli, A Goldstein, M W Hunkapiller, L E Hood
PMCID: PMC346912  PMID: 6127674

Abstract

A 4,000-dalton dynorphin was isolated from porcine pituitary. It has 32 amino acids (Mr = 3,986), with the previously described heptadecapeptide (now called dynorphin A) at its amino terminus and a related tridecapeptide, dynorphin B, at its carboxyl terminus. The two peptides are separated by the "processing signal" Lys-Arg.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chavkin C., Goldstein A. Demonstration of a specific dynorphin receptor in guinea pig ileum myenteric plexus. Nature. 1981 Jun 18;291(5816):591–593. doi: 10.1038/291591a0. [DOI] [PubMed] [Google Scholar]
  2. Chavkin C., Goldstein A. Specific receptor for the opioid peptide dynorphin: structure--activity relationships. Proc Natl Acad Sci U S A. 1981 Oct;78(10):6543–6547. doi: 10.1073/pnas.78.10.6543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chavkin C., James I. F., Goldstein A. Dynorphin is a specific endogenous ligand of the kappa opioid receptor. Science. 1982 Jan 22;215(4531):413–415. doi: 10.1126/science.6120570. [DOI] [PubMed] [Google Scholar]
  4. Comb M., Seeburg P. H., Adelman J., Eiden L., Herbert E. Primary structure of the human Met- and Leu-enkephalin precursor and its mRNA. Nature. 1982 Feb 25;295(5851):663–666. doi: 10.1038/295663a0. [DOI] [PubMed] [Google Scholar]
  5. Ghazarossian V. E., Chavkin C., Goldstein A. A specific radioimmunoassay for the novel opioid peptide dynorphin. Life Sci. 1980 Jul 7;27(1):75–86. doi: 10.1016/0024-3205(80)90021-1. [DOI] [PubMed] [Google Scholar]
  6. Goldstein A., Fischli W., Lowney L. I., Hunkapiller M., Hood L. Porcine pituitary dynorphin: complete amino acid sequence of the biologically active heptadecapeptide. Proc Natl Acad Sci U S A. 1981 Nov;78(11):7219–7223. doi: 10.1073/pnas.78.11.7219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goldstein A., Schulz R. Morphine-tolerant longitudinal muscle strip from guinea-pig ileum. Br J Pharmacol. 1973 Aug;48(4):655–666. doi: 10.1111/j.1476-5381.1973.tb08254.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldstein A., Tachibana S., Lowney L. I., Hunkapiller M., Hood L. Dynorphin-(1-13), an extraordinarily potent opioid peptide. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6666–6670. doi: 10.1073/pnas.76.12.6666. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gubler U., Seeburg P., Hoffman B. J., Gage L. P., Udenfriend S. Molecular cloning establishes proenkephalin as precursor of enkephalin-containing peptides. Nature. 1982 Jan 21;295(5846):206–208. doi: 10.1038/295206a0. [DOI] [PubMed] [Google Scholar]
  10. Hewick R. M., Hunkapiller M. W., Hood L. E., Dreyer W. J. A gas-liquid solid phase peptide and protein sequenator. J Biol Chem. 1981 Aug 10;256(15):7990–7997. [PubMed] [Google Scholar]
  11. Jacobs J. W., Goodman R. H., Chin W. W., Dee P. C., Habener J. F., Bell N. H., Potts J. T., Jr Calcitonin messenger RNA encodes multiple polypeptides in a single precursor. Science. 1981 Jul 24;213(4506):457–459. doi: 10.1126/science.6264603. [DOI] [PubMed] [Google Scholar]
  12. Kangawa K., Minamino N., Chino N., Sakakibara S., Matsuo H. The complete amino acid sequence of alpha-neo-endorphin. Biochem Biophys Res Commun. 1981 Apr 15;99(3):871–878. doi: 10.1016/0006-291x(81)91244-4. [DOI] [PubMed] [Google Scholar]
  13. Kilpatrick D. L., Taniguchi T., Jones B. N., Stern A. S., Shively J. E., Hullihan J., Kimura S., Stein S., Udenfriend S. A highly potent 3200-dalton adrenal opioid peptide that contains both a [Met]- and [Leu]enkephalin sequence. Proc Natl Acad Sci U S A. 1981 May;78(5):3265–3268. doi: 10.1073/pnas.78.5.3265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Lord J. A., Waterfield A. A., Hughes J., Kosterlitz H. W. Endogenous opioid peptides: multiple agonists and receptors. Nature. 1977 Jun 9;267(5611):495–499. doi: 10.1038/267495a0. [DOI] [PubMed] [Google Scholar]
  16. Lund P. K., Goodman R. H., Dee P. C., Habener J. F. Pancreatic preproglucagon cDNA contains two glucagon-related coding sequences arranged in tandem. Proc Natl Acad Sci U S A. 1982 Jan;79(2):345–349. doi: 10.1073/pnas.79.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A. C., Cohen S. N., Numa S. Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature. 1979 Mar 29;278(5703):423–427. doi: 10.1038/278423a0. [DOI] [PubMed] [Google Scholar]
  18. Noda M., Furutani Y., Takahashi H., Toyosato M., Hirose T., Inayama S., Nakanishi S., Numa S. Cloning and sequence analysis of cDNA for bovine adrenal preproenkephalin. Nature. 1982 Jan 21;295(5846):202–206. doi: 10.1038/295202a0. [DOI] [PubMed] [Google Scholar]
  19. Tachibana S., Araki K., Ohya S., Yoshida S. Isolation and structure of dynorphin, an opioid peptide, from porcine duodenum. Nature. 1982 Jan 28;295(5847):339–340. doi: 10.1038/295339a0. [DOI] [PubMed] [Google Scholar]
  20. Weber E., Roth K. A., Barchas J. D. Immunohistochemical distribution of alpha-neo-endorphin/dynorphin neuronal systems in rat brain: evidence for colocalization. Proc Natl Acad Sci U S A. 1982 May;79(9):3062–3066. doi: 10.1073/pnas.79.9.3062. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wüster M., Schulz R., Herz A. Highly specific opiate receptors for dynorphin-(1-13) in the mouse vas deferens. Eur J Pharmacol. 1980 Mar 21;62(2-3):235–236. doi: 10.1016/0014-2999(80)90283-6. [DOI] [PubMed] [Google Scholar]

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