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. 1982 Sep;79(18):5503–5507. doi: 10.1073/pnas.79.18.5503

Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome.

R Callahan, W Drohan, S Tronick, J Schlom
PMCID: PMC346932  PMID: 6291047

Abstract

Sequences related to the mouse mammary tumor virus (MMTV) genome have been detected in fragments of restricted human cellular DNA. These results were obtained by using recombinant DNA containing the MMTV proviral genome and lowering the stringency of blot-hybridization conditions. The MMTV genome also reacts with unique families of fragments in restricted cellular DNA from other mammalian species but not with salmon sperm DNA. A clone that reacted with labeled MMTV proviral DNA was selected from a human DNA library in Charon 4A. Under stringent conditions, a 3.7-kilobase MMTV-related EcoRI fragment of this clone hybridized with many of the same EcoRI restriction fragments of human cellular DNA detectable with MMTV proviral DNA under low-stringency conditions. Specific fragments of the human clone were shown to contain sequences related to the molecularly cloned gag, pol, and env regions of the MMTV genome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen P. R., Tronick S. R., Aaronson S. A. Structural organization and biological activity of molecular clones of the integrated genome of a BALB/c mouse sarcoma virus. J Virol. 1981 Nov;40(2):431–439. doi: 10.1128/jvi.40.2.431-439.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Axel R., Schlom J., Spiegelman S. Presence in human breast cancer of RNA homologous to mouse mammary tumour virus RNA. Nature. 1972 Jan 7;235(5332):32–36. doi: 10.1038/235032a0. [DOI] [PubMed] [Google Scholar]
  3. Barbacid M., Long L. K., Aaronson S. A. Major structural proteins of type B, type C, and type D oncoviruses share interspecies antigenic determinants. Proc Natl Acad Sci U S A. 1980 Jan;77(1):72–76. doi: 10.1073/pnas.77.1.72. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bergmann D. G., Souza L. M., Baluda M. A. Vertebrate DNAs contain nucleotide sequences related to the transforming gene of avian myeloblastosis virus. J Virol. 1981 Nov;40(2):450–455. doi: 10.1128/jvi.40.2.450-455.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Bishop J. M., Quintrell N., Medeiros E., Varmus H. E. Of birds and mice and men: comments on the use of animal models and molecular hybridization in the search for human tumor viruses. Cancer. 1974 Oct;34(4 Suppl):suppl–suppl:1426. doi: 10.1002/1097-0142(197410)34:8+<1421::aid-cncr2820340813>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  6. Callahan R., Drohan W., Gallahan D., D'Hoostelaere L., Potter M. Novel class of mouse mammary tumor virus-related DNA sequences found in all species of Mus, including mice lacking the virus proviral genome. Proc Natl Acad Sci U S A. 1982 Jul;79(13):4113–4117. doi: 10.1073/pnas.79.13.4113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Callahan R., Kuff E. L., Lueders K. K., Birkenmeier E. Genetic relationship between the Mus cervicolor M432 retrovirus and the Mus Musculus intracisternal type A particle. J Virol. 1981 Dec;40(3):901–911. doi: 10.1128/jvi.40.3.901-911.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dalla-Favera R., Gelmann E. P., Gallo R. C., Wong-Staal F. A human onc gene homologous to the transforming gene (v-sis) of simian sarcoma virus. Nature. 1981 Jul 2;292(5818):31–35. doi: 10.1038/292031a0. [DOI] [PubMed] [Google Scholar]
  9. Das M. R., Mink M. M. Sequence homology of nucleic acids from human breast cancer cells and complementary DNA's from murine mammary tumor virus and Mason-Pfizer monkey virus. Cancer Res. 1979 Dec;39(12):5106–5113. [PubMed] [Google Scholar]
  10. Day N. K., Witkin S. S., Sarkar N. H., Kinne D., Jussawalla D. J., Levin A., Hsia C. C., Geller N., Good R. A. Antibodies reactive with murine mammary tumor virus in sera of patients with breast cancer: geographic and family studies. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2483–2487. doi: 10.1073/pnas.78.4.2483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Drohan W., Schlom J. Differential distribution of mouse mammary tumor virus-related sequences in the DNA's of rats. J Natl Cancer Inst. 1979 May;62(5):1279–1286. doi: 10.1093/jnci/62.5.1279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ellis R. W., Defeo D., Shih T. Y., Gonda M. A., Young H. A., Tsuchida N., Lowy D. R., Scolnick E. M. The p21 src genes of Harvey and Kirsten sarcoma viruses originate from divergent members of a family of normal vertebrate genes. Nature. 1981 Aug 6;292(5823):506–511. doi: 10.1038/292506a0. [DOI] [PubMed] [Google Scholar]
  13. Goff S. P., Gilboa E., Witte O. N., Baltimore D. Structure of the Abelson murine leukemia virus genome and the homologous cellular gene: studies with cloned viral DNA. Cell. 1980 Dec;22(3):777–785. doi: 10.1016/0092-8674(80)90554-1. [DOI] [PubMed] [Google Scholar]
  14. Greenberg J. R. The polyribosomal mRNA--protein complex is a dynamic structure. Proc Natl Acad Sci U S A. 1981 May;78(5):2923–2926. doi: 10.1073/pnas.78.5.2923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Gross-Bellard M., Oudet P., Chambon P. Isolation of high-molecular-weight DNA from mammalian cells. Eur J Biochem. 1973 Jul 2;36(1):32–38. doi: 10.1111/j.1432-1033.1973.tb02881.x. [DOI] [PubMed] [Google Scholar]
  16. Howard D. K., Schlom J. Isolation of host-range variants of mouse mammary tumor viruses that efficiently infect cells in vitro. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5718–5722. doi: 10.1073/pnas.75.11.5718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Lawn R. M., Fritsch E. F., Parker R. C., Blake G., Maniatis T. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA. Cell. 1978 Dec;15(4):1157–1174. doi: 10.1016/0092-8674(78)90043-0. [DOI] [PubMed] [Google Scholar]
  18. Martin M. A., Bryan T., McCutchan T. F., Chan H. W. Detection and cloning of murine leukemia virus-related sequences from African green monkey liver DNA. J Virol. 1981 Sep;39(3):835–844. doi: 10.1128/jvi.39.3.835-844.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Martin M. A., Bryan T., Rasheed S., Khan A. S. Identification and cloning of endogenous retroviral sequences present in human DNA. Proc Natl Acad Sci U S A. 1981 Aug;78(8):4892–4896. doi: 10.1073/pnas.78.8.4892. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mesa-Tejada R., Keydar I., Ramanarayanan M., Ohno T., Fenoglio C., Spiegelman S. Detection in human breast carcinomas of an antigen immunologically related to a group-specific antigen of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1529–1533. doi: 10.1073/pnas.75.3.1529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Morris V. L., Medeiros E., Ringold G. M., Bishop J. M., Varmus H. E. Comparison of mouse mammary tumor virus-specific DNA in inbred, wild and Asian mice, and in tumors and normal organs from inbred mice. J Mol Biol. 1977 Jul;114(1):73–91. doi: 10.1016/0022-2836(77)90284-4. [DOI] [PubMed] [Google Scholar]
  22. Ohno T., Mesa-Tejada R., Keydar I., Ramanarayanan M., Bausch J., Spiegelman S. Human breast carcinoma antigen is immunologically related to the polypeptide of the group-specific glycoprotein of mouse mammary tumor virus. Proc Natl Acad Sci U S A. 1979 May;76(5):2460–2464. doi: 10.1073/pnas.76.5.2460. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Phillips S. J., Birkenmeier E. H., Callahan R., Eicher E. M. Male and female mouse DNAs can be discriminated using retroviral probes. Nature. 1982 May 20;297(5863):241–243. doi: 10.1038/297241a0. [DOI] [PubMed] [Google Scholar]
  24. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  25. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  26. Spector D. H., Varmus H. E., Bishop J. M. Nucleotide sequences related to the transforming gene of avian sarcoma virus are present in DNA of uninfected vertebrates. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4102–4106. doi: 10.1073/pnas.75.9.4102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Srinivasan A., Reddy E. P., Aaronson S. A. Abelson murine leukemia virus: molecular cloning of infectious integrated proviral DNA. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2077–2081. doi: 10.1073/pnas.78.4.2077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vaidya A. B., Black M. M., Dion A. S., Moore D. H. Homology between human breast tumour RNA and mouse mammary tumour virus genome. Nature. 1974 Jun 7;249(457):565–567. doi: 10.1038/249565a0. [DOI] [PubMed] [Google Scholar]
  29. Williams B. G., Blattner F. R. Construction and characterization of the hybrid bacteriophage lambda Charon vectors for DNA cloning. J Virol. 1979 Feb;29(2):555–575. doi: 10.1128/jvi.29.2.555-575.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wong-Staal F., Dalla-Favera R., Franchini G., Gelmann E. P., Gallo R. C. Three distinct genes in human DNA related to the transforming genes of mammalian sarcoma retroviruses. Science. 1981 Jul 10;213(4504):226–228. doi: 10.1126/science.6264598. [DOI] [PubMed] [Google Scholar]
  31. Yamamoto K. R., Chandler V. L., Ross S. R., Ucker D. S., Ring J. C., Feinstein S. C. Integration and activity of mammary tumor virus genes: regulation by hormone receptors and chromosomal position. Cold Spring Harb Symp Quant Biol. 1981;45(Pt 2):687–697. doi: 10.1101/sqb.1981.045.01.086. [DOI] [PubMed] [Google Scholar]

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