Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Sep;79(18):5636–5640. doi: 10.1073/pnas.79.18.5636

Cellular analysis of specificity of antibodies and of delayed type hypersensitivity responses toward some structurally related synthetic antigens: Boosting is determined by specificity of T cells

Behnaz Parhami-Seren 1, Gideon Strassmann 1, Edna Mozes 1, Michael Sela 1
PMCID: PMC346959  PMID: 6982473

Abstract

The crossreactivity between the random synthetic polypeptide antigen poly(Tyr,Glu)-poly(DLAla)--poly(Lys) [(T,G)-A--L] and its ordered-sequence analogs (Tyr-Tyr-Glu-Glu)-poly(DLAla)--poly(Lys) [(T-T-G-G)-A--L] and (Tyr-Glu-Tyr-Glu)-poly(DLAla)--poly(Lys) [(T-G-T-G)-A--L] at the level of humoral and cellular responses was studied. For delayed type hypersensitivity responses, (T,G)-A--L-activated T cells could be challenged with the homologous antigen as well as with the ordered analogs. T cells activated by (T-T-G-G)-A--L could be challenged with either the homologous antigen or with (T,G)-A--L but not with (T-G-T-G)-A--L. Similarly, no cross stimulation was observed between (T-G-T-G)-A--L-activated cells and (T-T-G-G)-A--L, whereas (T,G)-A--L could challenge the latter cells to mediate significant responses. Similar but not identical cross reactions were observed when primed spleen cells or lymph nodes were transferred to irradiated recipients that were boosted for the production of antibodies. In contrast to observations at the level of cellular responses, (T-G-T-G)-A--L-primed spleen or lymph node cells could not be boosted with (T,G)-A--L for the production of detectable amounts of antibodies, although boosting with the homologous antigen resulted in significant levels of (T-G-T-G)-A--L-specific antibodies. Transfer experiments in which mixtures of T and B cells, each primed to a different ordered polypeptide antigen, were injected into irradiated recipients showed that successful cooperation occurs provided that the boost is given with the T-cell-specific antigen. The antibodies produced were specific to the antigen used for B-cell priming. The T-cell-B-cell collaboration probably occurs through specific determinants that are shared between the two antigens in which the ordered peptides are attached to the same multichain polymer and that are recognized by both the T and the B cells.

Keywords: T-cell-B-cell collaboration, random and ordered synthetic polypeptides, cellular response, humoral response

Full text

PDF
5636

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benacerraf B., McDevitt H. O. Histocompatibility-linked immune response genes. Science. 1972 Jan 21;175(4019):273–279. doi: 10.1126/science.175.4019.273. [DOI] [PubMed] [Google Scholar]
  2. Bolton A. E., Hunter W. M. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent. Biochem J. 1973 Jul;133(3):529–539. doi: 10.1042/bj1330529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eshhar Z., Strassmann G., Waks T., Mozes E. In vitro and in vivo induction of effector T cells mediating DTH responses to a protein and a synthetic polypeptide antigen. Cell Immunol. 1979 Oct;47(2):378–389. doi: 10.1016/0008-8749(79)90347-2. [DOI] [PubMed] [Google Scholar]
  4. Hunter R. Standardization of the chloramine-T method of protein iodination. Proc Soc Exp Biol Med. 1970 Mar;133(3):989–992. doi: 10.3181/00379727-133-34611. [DOI] [PubMed] [Google Scholar]
  5. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  6. Klinman N. R., Pickard A. R., Sigal N. H., Gearhart P. J., Metcalf E. S., Pierce S. K. Assessing B cell diversification by antigen receptor and precursor cell analysis. Ann Immunol (Paris) 1976 Jun-Jul;127(3-4):489–502. [PubMed] [Google Scholar]
  7. Mozes E., Schwartz M., Sela M. Antibody response of inbred mouse strains to ordered tetrapeptides of tyrosine and glutamic acid attached to multichain polyalanine or polyproline. Tyr-Tyr-Glu-Glu is a major determinant of the random poly-(Tyr, Glu)-polyDLAla--polyLys. J Exp Med. 1974 Aug 1;140(2):349–355. doi: 10.1084/jem.140.2.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mozes E., Shearer G. M. Genetic control of immune responses. Curr Top Microbiol Immunol. 1972;59:167–200. doi: 10.1007/978-3-642-65444-2_6. [DOI] [PubMed] [Google Scholar]
  9. NISONOFF A., MARKUS G., WISSLER F. C. Separation of univalent fragments of rabbit antibody by reduction of a single, labile disulphide bond. Nature. 1961 Jan 28;189:293–295. doi: 10.1038/189293a0. [DOI] [PubMed] [Google Scholar]
  10. Raff M. Theta isoantigen as a marker of thymus-derived lymphocytes in mice. Nature. 1969 Oct 25;224(5217):378–379. doi: 10.1038/224378a0. [DOI] [PubMed] [Google Scholar]
  11. SELA M., FUCHS S., ARNON R. Studies on the chemical basis of the antigenicity of proteins. 5. Synthesis, characterization and immunogenicity of some multichain and linear polypeptides containing tyrosine. Biochem J. 1962 Oct;85:223–235. doi: 10.1042/bj0850223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Schwartz M., Hooghe R. J., Mozes E., Sela M. Role of antigenic structure in cell to cell cooperation. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4184–4186. doi: 10.1073/pnas.73.11.4184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwartz M., Parhami B., Mozes E., Sela M. Change in specificity of antibodies to a random synthetic branched polypeptide in mice tolerant to its ordered analogs. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5286–5288. doi: 10.1073/pnas.76.10.5286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Strassmann G., Eshhar Z., Mozes E. Genetic regulation of delayed-type hypersensitivity responses to poly(LTyr,LGu)-poly(DLAla)--poly(LLys). I. Expression of the genetic defect at two phases of the immune process. J Exp Med. 1980 Feb 1;151(2):265–274. doi: 10.1084/jem.151.2.265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Strausbauch P. H., Tarrab R., Sulica A., Sela M. Stimulation in vivo and in vitro of anti-dinitrophenyl antibodies with homologous protein carriers devoid of hapten. J Immunol. 1972 Jan;108(1):236–245. [PubMed] [Google Scholar]
  16. Vadas M. A., Miller J. F., Gamble J., Whitelaw A. A radioisotopic method to measure delayed type hypersensitivity in the mouse. I. Studies in sensitized and normal mice. Int Arch Allergy Appl Immunol. 1975;49(5):670–692. doi: 10.1159/000231449. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES