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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Sep;79(18):5675–5679. doi: 10.1073/pnas.79.18.5675

Monoclonal radioimmunoassays for hepatitis B surface antigen: demonstration of hepatitis B virus DNA or related sequences in serum and viral epitopes in immune complexes.

D A Shafritz, H M Lieberman, K J Isselbacher, J R Wands
PMCID: PMC346967  PMID: 6182569

Abstract

High-affinity monoclonal IgM and IgG antibodies (anti-HBs) to hepatitis B surface antigen (HBsAg) have recently been developed and used by us in the construction of highly sensitive radioimmunoassays for the detection of HBsAg-associated determinants in serum. We now report that selected serum samples demonstrating monoclonal immunoreactive material also contain nucleic acid sequences complementary to hepatitis B virus (HBV) DNA by molecular hybridization analysis. In addition, monoclonal radioimmunoassays can detect viral determinants in HBsAg-anti-HBs immune complexes formed in anti-HBs excess; such determinants are undetectable by commercial radioimmunoassay. These and our previous studies suggest that there are HBV or "HBV-related" agents present in human serum that are detected by monoclonal antibodies but are not identified by conventional polyvalent antibodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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