Abstract
A series of 12 monoclonal antibodies has been prepared against crude or purified individual bovine neurofilament (NF) triplet proteins (designated as P68, P150, and P200). Six of the antibodies recognized unique determinants on individual NF subunits. The other six recognized shared determinants on at least two NF triplet proteins. Six of the antibodies recognized separated, immobilized individual NF in nitrocellulose paper. By the peroxidase-antiperoxidase technique, all of the antibodies stained neurons and their processes in a manner identical to the well-known distribution of NF in both the central and peripheral nervous system. These results strongly suggest that the NF subunits are distinct but related proteins and that the smaller NF subunits (P68 and P150) are not derived from larger NF proteins.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Autilio-Gambetti L., Velasco M. E., Sipple J., Gambetti P. Immunochemical characterization of antisera to rat neurofilament subunits. J Neurochem. 1981 Nov;37(5):1260–1265. doi: 10.1111/j.1471-4159.1981.tb04676.x. [DOI] [PubMed] [Google Scholar]
- Berod A., Hartman B. K., Pujol J. F. Importance of fixation in immunohistochemistry: use of formaldehyde solutions at variable pH for the localization of tyrosine hydroxylase. J Histochem Cytochem. 1981 Jul;29(7):844–850. doi: 10.1177/29.7.6167611. [DOI] [PubMed] [Google Scholar]
- Franke W. W., Schmid E., Winter S., Osborn M., Weber K. Widespread occurrence of intermediate-sized filaments of the vimentin-type in cultured cells from diverse vertebrates. Exp Cell Res. 1979 Oct 1;123(1):25–46. doi: 10.1016/0014-4827(79)90418-x. [DOI] [PubMed] [Google Scholar]
- Hogue-Angeletti R. A., Wu H. L., Schlaepfer W. W. Preparative separation and amino acid composition of neurofilament triplet proteins. J Neurochem. 1982 Jan;38(1):116–120. doi: 10.1111/j.1471-4159.1982.tb10861.x. [DOI] [PubMed] [Google Scholar]
- Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Lazarides E., Balzer D. R., Jr Specificity of desmin to avian and mammalian muscle cells. Cell. 1978 Jun;14(2):429–438. doi: 10.1016/0092-8674(78)90128-9. [DOI] [PubMed] [Google Scholar]
- Liem R. K., Yen S. H., Salomon G. D., Shelanski M. L. Intermediate filaments in nervous tissues. J Cell Biol. 1978 Dec;79(3):637–645. doi: 10.1083/jcb.79.3.637. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlaepfer W. W., Lee V., Wu H. L. Assessment of immunological properties of neurofilament triplet proteins. Brain Res. 1981 Dec 7;226(1-2):259–272. doi: 10.1016/0006-8993(81)91098-2. [DOI] [PubMed] [Google Scholar]
- Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yen S. H., Fields K. L. Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol. 1981 Jan;88(1):115–126. doi: 10.1083/jcb.88.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]