Abstract
The unusual NH2-terminal blocking group of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein was found to be amide-linked n-tetradecanoic acid by gas chromatographic-, direct chemical ionization-, and fast atom bombardment-mass spectrometry. In addition, fast atom bombardment mass spectrometry revealed the presence of an additional alanine which had been overlooked when the original sequence was determined. The corrected and completed NH2-terminal sequence of the 350-amino acid catalytic subunit is CH3(CH2)12CONH-Gly-Asn-Ala-Ala-Ala-Ala-Lys.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barber M., Bordoli R. S., Garner G. V., Gordon D. B., Sedgwick R. D., Tetler L. W., Tyler A. N. Fast-atom-bombardment mass spectra of enkephalins. Biochem J. 1981 Aug 1;197(2):401–404. doi: 10.1042/bj1970401. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Corbin J. D., Sugden P. H., Lincoln T. M., Keely S. L. Compartmentalization of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in heart tissue. J Biol Chem. 1977 Jun 10;252(11):3854–3861. [PubMed] [Google Scholar]
- Hantke K., Braun V. Covalent binding of lipid to protein. Diglyceride and amide-linked fatty acid at the N-terminal end of the murein-lipoprotein of the Escherichia coli outer membrane. Eur J Biochem. 1973 Apr;34(2):284–296. doi: 10.1111/j.1432-1033.1973.tb02757.x. [DOI] [PubMed] [Google Scholar]
- Jungmann R. A., Russell D. H. Cyclic AMP, cyclic AMP-dependent protein kinase, and the regulation of gene expression. Life Sci. 1977 Jun 1;20(11):1787–1797. doi: 10.1016/0024-3205(77)90213-2. [DOI] [PubMed] [Google Scholar]
- Peters K. A., Demaille J. G., Fischer E. H. Adenosine 3':5'-monophosphate dependent protein kinase from bovine heart. Characterization of the catalytic subunit. Biochemistry. 1977 Dec 27;16(26):5691–5697. doi: 10.1021/bi00645a007. [DOI] [PubMed] [Google Scholar]
- Rubin C. S., Rosenfeld R. D., Rosen O. M. Studies on the orientation of cyclic AMP-dependent protein kinase in human erythrocyte membranes. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3735–3738. doi: 10.1073/pnas.70.12.3735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schlesinger M. J., Magee A. I., Schmidt M. F. Fatty acid acylation of proteins in cultured cells. J Biol Chem. 1980 Nov 10;255(21):10021–10024. [PubMed] [Google Scholar]
- Schmidt M. F., Bracha M., Schlesinger M. J. Evidence for covalent attachment of fatty acids to Sindbis virus glycoproteins. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1687–1691. doi: 10.1073/pnas.76.4.1687. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shoji S., Parmelee D. C., Wade R. D., Kumar S., Ericsson L. H., Walsh K. A., Neurath H., Long G. L., Demaille J. G., Fischer E. H. Complete amino acid sequence of the catalytic subunit of bovine cardiac muscle cyclic AMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1981 Feb;78(2):848–851. doi: 10.1073/pnas.78.2.848. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takio K., Smith S. B., Krebs E. G., Walsh K. A., Titani K. Primary structure of the regulatory subunit of type II cAMP-dependent protein kinase from bovine cardiac muscle. Proc Natl Acad Sci U S A. 1982 Apr;79(8):2544–2548. doi: 10.1073/pnas.79.8.2544. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uy R., Wold F. Posttranslational covalent modification of proteins. Science. 1977 Dec 2;198(4320):890–896. doi: 10.1126/science.337487. [DOI] [PubMed] [Google Scholar]
- Zilberstein A., Snider M. D., Porter M., Lodish H. F. Mutants of vesicular stomatitis virus blocked at different stages in maturation of the viral glycoprotein. Cell. 1980 Sep;21(2):417–427. doi: 10.1016/0092-8674(80)90478-x. [DOI] [PubMed] [Google Scholar]