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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Oct;79(20):6242–6245. doi: 10.1073/pnas.79.20.6242

Expression of the alpha subunit of human chorionic gonadotropin is specifically correlated with tumorigenic expression in human cell hybrids.

E J Stanbridge, S W Rosen, H H Sussman
PMCID: PMC347096  PMID: 6959112

Abstract

The expression of HeLa parent phenotype protein markers, the alpha subunit of human chorionic gonadotropin and placental alkaline phosphatase isoenzymes, has been evaluated in paired tumorigenic and nontumorigenic HeLa-fibroblast human cell hybrids. Both of these proteins have been used clinically as markers of malignancy. The results showed that both are expressed in the hybrids. Expression of the gonadotropin subunit in the hybrids is specifically correlated with tumorigenicity; the placental alkaline phosphatase isoenzyme showed no such correlation and was expressed in both tumorigenic and nontumorigenic hybrids.

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Selected References

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  1. ARMITAGE P., DOLL R. The age distribution of cancer and a multi-stage theory of carcinogenesis. Br J Cancer. 1954 Mar;8(1):1–12. doi: 10.1038/bjc.1954.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bahl O. P. Human chorionic gonadotropin. I. Purification and physicochemical properties. J Biol Chem. 1969 Feb 25;244(4):567–574. [PubMed] [Google Scholar]
  3. Barrett J. C., Ts'o P. O. Evidence for the progressive nature of neoplastic transformation in vitro. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3761–3765. doi: 10.1073/pnas.75.8.3761. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. COX R. P., MACLEOD C. M. Alkaline phosphatase content and the effects of prednisolone on mammalian cells in culture. J Gen Physiol. 1962 Jan;45:439–485. doi: 10.1085/jgp.45.3.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chou J. Y., Robinson J. C., Wang S. S. Effects of sodium butyrate on synthesis of human chorionic gonadotrophin in trophoblastic and non-trophoblastic tumours. Nature. 1977 Aug 11;268(5620):543–544. doi: 10.1038/268543a0. [DOI] [PubMed] [Google Scholar]
  6. Chou J. Y., Wang S. S., Robinson J. C. Regulation of the synthesis of human chorionic gonadotrophin by 5-bromo-2'-deoxyuridine and dibutyryl cyclic AMP in trophoblastic and nontrophoblastic tumor cells. J Clin Endocrinol Metab. 1978 Jul;47(1):46–51. doi: 10.1210/jcem-47-1-46. [DOI] [PubMed] [Google Scholar]
  7. Comings D. E. A general theory of carcinogenesis. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3324–3328. doi: 10.1073/pnas.70.12.3324. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Der C. J., Stanbridge E. J. Alterations in the extracellular matrix organization associated with the reexpression of tumorigenicity in human cell hybrids. Int J Cancer. 1980 Oct 15;26(4):451–459. doi: 10.1002/ijc.2910260410. [DOI] [PubMed] [Google Scholar]
  9. Fishman W. H. Perspectives on alkaline phosphatase isoenzymes. Am J Med. 1974 May;56(5):617–650. doi: 10.1016/0002-9343(74)90631-7. [DOI] [PubMed] [Google Scholar]
  10. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Griffin M. J., Price G. H., Bazzell K. L. A study of adenosine 3':5'-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase. Arch Biochem Biophys. 1974 Oct;164(2):619–623. doi: 10.1016/0003-9861(74)90073-3. [DOI] [PubMed] [Google Scholar]
  12. Hamilton T. A., Tin A. W., Sussman H. H., Weintraub B. D., Rosen S. W. Independent expression of alpha-HCG and placental alkaline phosphatase in clonal human cell lines. Oncodev Biol Med. 1980;1(3):161–167. [PubMed] [Google Scholar]
  13. Hamilton T. A., Wada H. G., Sussman H. H. Human placental cell surface antigens:expression by cultured cells of diverse phenotypic origin. J Supramol Struct. 1979;11(4):503–515. doi: 10.1002/jss.400110409. [DOI] [PubMed] [Google Scholar]
  14. Harris H., Miller O. J., Klein G., Worst P., Tachibana T. Suppression of malignancy by cell fusion. Nature. 1969 Jul 26;223(5204):363–368. doi: 10.1038/223363a0. [DOI] [PubMed] [Google Scholar]
  15. Koyama H., Ono T. Induction of alkaline phosphatase by 5-bromodeoxyuridine in a hybrid line between mouse and Chinese hamster in culture. Exp Cell Res. 1971 Dec;69(2):468–470. doi: 10.1016/0014-4827(71)90257-6. [DOI] [PubMed] [Google Scholar]
  16. Kucherlapati R., Shin S. I. Genetic control of tumorigenicity in interspecific mammalian cell hybrids. Cell. 1979 Mar;16(3):639–648. doi: 10.1016/0092-8674(79)90037-0. [DOI] [PubMed] [Google Scholar]
  17. Lieblich J. M., Rosen S. W., Weintraub B. D., Sindelar W. F., Tralka T. S., Rabson A. S. Maintenance of ectopic chorionic gonadotropin and alpha subunit secretion by a human lung cancer cell line (ChaGo) transplanted into nude mice. J Natl Cancer Inst. 1977 Oct;59(4):1285–1289. doi: 10.1093/jnci/59.4.1285. [DOI] [PubMed] [Google Scholar]
  18. Lieblich J. M., Weintraub B. D., Rosen S. W., Chou J. Y., Robinson J. C. HeLa cells secrete alpha subunit of glycoprotein tropic hormones. Nature. 1976 Apr 8;260(5551):530–532. doi: 10.1038/260530a0. [DOI] [PubMed] [Google Scholar]
  19. Ludueña M. A., Iverson G. M., Sussman H. H. Expression of liver and placental alkaline phosphatases in Chang liver cells. J Cell Physiol. 1977 Apr;91(1):119–129. doi: 10.1002/jcp.1040910112. [DOI] [PubMed] [Google Scholar]
  20. Mehdi S. Q., Nussey S. S. A radio-ligand receptor assay for the long-acting thyroid stimulator. Inhibition by the long-acting thyroid stimulator of the binding of radioiodinated thyroid-stimulating hormone to human thyroid membranes. Biochem J. 1975 Jan;145(1):105–111. doi: 10.1042/bj1450105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rubin H. Is somatic mutation the major mechanism of malignant transformation? J Natl Cancer Inst. 1980 May;64(5):995–1000. [PubMed] [Google Scholar]
  22. Ruddon R. W., Bryan A. H., Meade-Cobun K. S., Pollack V. A. Production of human chorionic gonadotropin and its subunits by human tumors growing in nude mice. Cancer Res. 1980 Nov;40(11):4007–4012. [PubMed] [Google Scholar]
  23. Russell W. C., Newman C., Williamson D. H. A simple cytochemical technique for demonstration of DNA in cells infected with mycoplasmas and viruses. Nature. 1975 Feb 6;253(5491):461–462. doi: 10.1038/253461a0. [DOI] [PubMed] [Google Scholar]
  24. Sager R., Kovac P. E. Genetic analysis of tumorigenesis: IV. Chromosome reduction and marker segregation in progeny clones from Chinese hamster cell hybrids. Somatic Cell Genet. 1979 Jul;5(4):491–502. doi: 10.1007/BF01538883. [DOI] [PubMed] [Google Scholar]
  25. Stanbridge E. J., Ceredig R. Growth-regulatory control of human cell hybrids in nude mice. Cancer Res. 1981 Feb;41(2):573–580. [PubMed] [Google Scholar]
  26. Stanbridge E. J., Der C. J., Doersen C. J., Nishimi R. Y., Peehl D. M., Weissman B. E., Wilkinson J. E. Human cell hybrids: analysis of transformation and tumorigenicity. Science. 1982 Jan 15;215(4530):252–259. doi: 10.1126/science.7053574. [DOI] [PubMed] [Google Scholar]
  27. Stanbridge E. J., Flandermeyer R. R., Daniels D. W., Nelson-Rees W. A. Specific chromosome loss associated with the expression of tumorigenicity in human cell hybrids. Somatic Cell Genet. 1981 Nov;7(6):699–712. doi: 10.1007/BF01538758. [DOI] [PubMed] [Google Scholar]
  28. Stanbridge E. J. Suppression of malignancy in human cells. Nature. 1976 Mar 4;260(5546):17–20. doi: 10.1038/260017a0. [DOI] [PubMed] [Google Scholar]
  29. Stanbridge E. J., Wilkinson J. Analysis of malignancy in human cells: malignant and transformed phenotypes are under separate genetic control. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1466–1469. doi: 10.1073/pnas.75.3.1466. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stanbridge E. J., Wilkinson J. Dissociation of anchorage independence form tumorigenicity in human cell hybrids. Int J Cancer. 1980 Jul 15;26(1):1–8. doi: 10.1002/ijc.2910260102. [DOI] [PubMed] [Google Scholar]
  31. Sussman H. H., Small P. A., Jr, Cotlove E. Human alkaline phosphatase. Immunochemical identification of organ-specific isoenzymes. J Biol Chem. 1968 Jan 10;243(1):160–166. [PubMed] [Google Scholar]
  32. Swaminathan N., Bahl O. P. Dissociation and recombination of the subunits of human chorionic gonadotropin. Biochem Biophys Res Commun. 1970 Jul 27;40(2):422–427. doi: 10.1016/0006-291x(70)91026-0. [DOI] [PubMed] [Google Scholar]
  33. Tashjian A. H., Jr, Weintraub B. D., Barowsky N. J., Rabson A. S., Rosen S. W. Subunits of human chorionic gonadotropin: unbalanced synthesis and secretion by clonal cell strains derived from a bronchogenic carcinoma. Proc Natl Acad Sci U S A. 1973 May;70(5):1419–1422. doi: 10.1073/pnas.70.5.1419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Tralka T. S., Rosen S. W., Weintraub B. D., Lieblich J. M., Engel L. W., Wetzel B. K., Kingsbury E. W., Rabson A. S. Ultrastructural concomitants of sodium butyrate-enhanced ectopic production of chorionic gonadotropin and its alpha subunit human bronchogenic carcinoma (ChaGo) cells. J Natl Cancer Inst. 1979 Jan;62(1):45–61. [PubMed] [Google Scholar]
  35. Vaitukaitis J. L., Ross G. T., Braunstein G. D., Rayford P. L. Gonadotropins and their subunits: basic and clinical studies. Recent Prog Horm Res. 1976;32:289–331. doi: 10.1016/b978-0-12-571132-6.50019-1. [DOI] [PubMed] [Google Scholar]
  36. Wilson P. D., Benham F., Franks L. M. Alkaline phosphatase phenotypes in tumour and non-tumour cell lines: not an invariable marker for neoplastic transformation. Cell Biol Int Rep. 1977 May;1(3):229–238. doi: 10.1016/0309-1651(77)90047-9. [DOI] [PubMed] [Google Scholar]

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