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. 1982 Oct;79(20):6260–6264. doi: 10.1073/pnas.79.20.6260

Structures of the genes for the β and ε subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signal

Gerard Zurawski *,†, Warwick Bottomley *, Paul R Whitfeld *,‡
PMCID: PMC347100  PMID: 16593238

Abstract

A 2,4-kilobase-pair region of spinach chloroplast DNA adjacent to the gene for the large subunit of ribulosebisphosphate carboxylase has been analyzed by RNA hybridization, in vitro transcription/translation, and DNA sequence determination. The analysis indicates that this region carries the genes for the β and ε subunits of chloroplast ATPase and that the two genes are cotranscribed into a dicistronic mRNA with 4-base-pair overlap between the stop codon of the β-subunit gene and the start codon of the ε-subunit gene. The ATPase and carboxylase genes are transcribed divergently with respect to each other. The deduced amino acid sequences of the β and ε subunits from spinach show 67% and 26% homology, respectively, with the published sequences of the β and ε subunits of Escherichia coli ATPase.

Keywords: chloroplast DNA, nucleotide sequence, amino acid sequence, homology with Escherichia coli ATPase, overlapping genes

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Selected References

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