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. 1982 Nov;79(21):6434–6437. doi: 10.1073/pnas.79.21.6434

Amino acid sequence of a basic blue protein from cucumber seedlings

M Murata *, G S Begg †, F Lambrou †, B Leslie †, R J Simpson †, H C Freeman *,‡, F J Morgan †
PMCID: PMC347140  PMID: 16593245

Abstract

The amino acid sequence of a type 1 copper protein, the 96-residue basic blue protein from cucumber seedlings, has been determined by Edman degradation of the intact molecule and of fragments produced by cleavage with cyanogen bromide and with trypsin. The cucumber basic blue protein shows a marked sequence homology with stellacyanin, and to a smaller degree with plastocyanin and azurin. The known copper ligands of plastocyanin and azurin (corresponding to histidine-37, cysteine-84, histidine-87, and methionine-92 in plastocyanin) are present in the cucumber basic blue protein. However, the latter also contains a half-cystine residue analogous to the suggested fourth ligand of stellacyanin, where methionine is absent.

Keywords: copper proteins, azurin, plastocyanin, stellacyanin, sequence homology

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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