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. Author manuscript; available in PMC: 2012 Oct 15.
Published in final edited form as: Oncogene. 2007 Sep 10;27(10):1412–1420. doi: 10.1038/sj.onc.1210784

Figure 1.

Figure 1

E4-ORF1 exists as a monomer and trimer. (a) E4-ORF1 elutes as a monomer and trimer by SEC. 293 T cells were transfected with pGW1 encoding wt E4-ORF1 or His-dUTPase. Extracts in NETN buffer were fractionated by SEC with elution buffer lacking detergent and blotted with E4-ORF1 or His antibody. Fractions lacking E4-ORF1 or dUTPase signal are not shown. Arrows mark elution peaks of size markers. (b) Stringent detergents disrupt E4-ORF1 trimers and DOC reverses this effect. Extracts in RIPA buffer were fractionated by SEC with elution buffer lacking or containing DOC and analyzed as described in (a).