Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Nov;79(21):6646–6650. doi: 10.1073/pnas.79.21.6646

Antigen-specific human T-cell hybridomas with helper activity.

E C DeFreitas, S Vella, A Linnenbach, C Zmijewski, H Koprowski, C M Croce
PMCID: PMC347185  PMID: 6983073

Abstract

Human T cell hybridomas were produced by fusing the hypoxanthine phosphoribosyltransferase-deficient line of the human T cell lymphoma Jurkat with a continuous line of normal human T cells specific for tetanus toxoid (TeT). The hybridomas were selected for their ability to produce interleukin 2 after exposure to TeT on semiautologous monocytes and for their ability to bind to TeT-pulsed semiautologous monocytes. These antigen-specific T hybridomas demonstrated potent helper activity for semiautologous B cells as determined by the production of high levels of anti-TeT antibody in vitro.

Full text

PDF
6646

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Schreier M. H., Melchers F. T-cell-dependent B-cell stimulation is H-2 restricted and antigen dependent only at the resting B-cell level. Proc Natl Acad Sci U S A. 1980 Mar;77(3):1612–1616. doi: 10.1073/pnas.77.3.1612. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bergholtz B. O., Thorsby E. Macrophage-dependent response of immune human T lymphocytes to PPD in vitro. Influence of HLA-D histocompatibility. Scand J Immunol. 1977;6(8):779–786. doi: 10.1111/j.1365-3083.1977.tb02151.x. [DOI] [PubMed] [Google Scholar]
  3. Corte G., Mingari M. C., Moretta A., Damiani G., Moretta L., Bargellesi A. Human T cell subpopulations defined by a monoclonal antibody. I. A small subset is responsible for proliferation to allogeneic cells or to soluble antigens and for helper activity for B cell differentiation. J Immunol. 1982 Jan;128(1):16–19. [PubMed] [Google Scholar]
  4. Croce C. M., Kieba I., Koprowski H. Unidirectional loss of human chromosomes in rat-human hybrids. Exp Cell Res. 1973 Jun;79(2):461–463. doi: 10.1016/0014-4827(73)90470-9. [DOI] [PubMed] [Google Scholar]
  5. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  6. Gillis S., Watson J. Biochemical and biological characterization of lymphocyte regulatory molecules. V. Identification of an interleukin 2-producing human leukemia T cell line. J Exp Med. 1980 Dec 1;152(6):1709–1719. doi: 10.1084/jem.152.6.1709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grillot-Courvalin C., Brouet J. C., Berger R., Bernheim A. Establishment of a human T-cell hybrid line with suppressive activity. Nature. 1981 Aug 27;292(5826):844–845. doi: 10.1038/292844a0. [DOI] [PubMed] [Google Scholar]
  8. Heijnen C. J., UytdeHaag F., Gmelig-Meyling F. H., Ballieux R. E. Localization of human antigen-specific helper and suppressor function in distinct T-cell subpopulations. Cell Immunol. 1979 Mar 15;43(2):282–292. doi: 10.1016/0008-8749(79)90173-4. [DOI] [PubMed] [Google Scholar]
  9. Hoffmann M. K. Antigen-specific induction and regulation of antibody synthesis in cultures of human peripheral blood mononuclear cells. Proc Natl Acad Sci U S A. 1980 Feb;77(2):1139–1143. doi: 10.1073/pnas.77.2.1139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Irigoyen O., Rizzolo P. V., Thomas Y., Rogozinski L., Chess L. Generation of functional human T cell hybrids. J Exp Med. 1981 Dec 1;154(6):1827–1837. doi: 10.1084/jem.154.6.1827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jondal M., Holm G., Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972 Aug 1;136(2):207–215. doi: 10.1084/jem.136.2.207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kappler J. W., Skidmore B., White J., Marrack P. Antigen-inducible, H-2-restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J Exp Med. 1981 May 1;153(5):1198–1214. doi: 10.1084/jem.153.5.1198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kontiainen S., Simpson E., Bohrer E., Beverley P. C., Herzenberg L. A., Fitzpatrick W. C., Vogt P., Torano A., McKenzie I. F., Feldmann M. T-cell lines producing antigen-specific suppressor factor. Nature. 1978 Aug 3;274(5670):477–480. doi: 10.1038/274477a0. [DOI] [PubMed] [Google Scholar]
  14. Kung P. C., Talle M. A., DeMaria M. E., Butler M. S., Lifter J., Goldstein G. Strategies for generating monoclonal antibodies defining human t-lymphocyte differentiation antigens. Transplant Proc. 1980 Sep;12(3 Suppl 1):141–146. [PubMed] [Google Scholar]
  15. Kurnick J. T., Grönvik K. O., Kimura A. K., Lindblom J. B., Skoog V. T., Sjöberg O., Wigzell H. Long term growth in vitro of human T cell blasts with maintenance of specificity and function. J Immunol. 1979 Apr;122(4):1255–1260. [PubMed] [Google Scholar]
  16. Larsson E. L., Iscove N. N., Coutinho A. Two distinct factors are required for induction of T-cell growth. Nature. 1980 Feb 14;283(5748):664–666. doi: 10.1038/283664a0. [DOI] [PubMed] [Google Scholar]
  17. Leibson H. J., Marrack P., Kappler J. W. B cell helper factors. I. Requirement for both interleukin 2 and another 40,000 mol wt factor. J Exp Med. 1981 Nov 1;154(5):1681–1693. doi: 10.1084/jem.154.5.1681. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lipscomb M. F., Ben-Sasson S. Z., Uhr J. W. Specific binding of T lymphocytes to macrophages. I. Kinetics of binding. J Immunol. 1977 May;118(5):1748–1754. [PubMed] [Google Scholar]
  19. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Morgan D. A., Ruscetti F. W., Gallo R. Selective in vitro growth of T lymphocytes from normal human bone marrows. Science. 1976 Sep 10;193(4257):1007–1008. doi: 10.1126/science.181845. [DOI] [PubMed] [Google Scholar]
  21. Okada M., Yoshimura N., Kaieda T., Yamamura Y., Kishimoto T. Establishment and characterization of human T hybrid cells secreting immunoregulatory molecules. Proc Natl Acad Sci U S A. 1981 Dec;78(12):7717–7721. doi: 10.1073/pnas.78.12.7717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Pichler W. J., Lum L., Broder S. Fc-receptors on human T lymphocytes. I. Transition of Tgamma to Tmu cells. J Immunol. 1978 Oct;121(4):1540–1548. [PubMed] [Google Scholar]
  23. Schrier R. D., Skidmore B. J., Kurnick J. T., Goldstine S. N., Chiller J. M. Propagation of antigen-specific T cell helper function in vitro. J Immunol. 1979 Dec;123(6):2525–2531. [PubMed] [Google Scholar]
  24. Seabright M. A rapid banding technique for human chromosomes. Lancet. 1971 Oct 30;2(7731):971–972. doi: 10.1016/s0140-6736(71)90287-x. [DOI] [PubMed] [Google Scholar]
  25. Sredni B., Volkman D., Schwartz R. H., Fauci A. S. Antigen-specific human T-cell clones: development of clones requiring HLA-DR-compatible presenting cells for stimulation in presence of antigen. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1858–1862. doi: 10.1073/pnas.78.3.1858. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Swain S. L., Dennert G., Warner J. F., Dutton R. W. Culture supernatants of a stimulated T-cell line have helper activity that acts synergistically with interleukin 2 in the response of B cells to antigen. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2517–2521. doi: 10.1073/pnas.78.4.2517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Zollinger W. D., Dalrymple J. M., Artenstein M. S. Analysis of parameters affecting the solid phase radioimmunoassay quantitation of antibody to meningococcal antigens. J Immunol. 1976 Nov;117(5 PT2):1788–1798. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES