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. 1982 Nov;79(22):6903–6907. doi: 10.1073/pnas.79.22.6903

Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthase

C J Howe *, A D Auffret †, A Doherty *,‡, C M Bowman §, T A Dyer §, J C Gray *
PMCID: PMC347242  PMID: 16593250

Abstract

The proton-translocating subunit of wheat chloroplast ATP synthase is encoded by a chloroplast gene that has been accurately mapped and whose nucleotide sequence has been determined. The predicted sequence of 81 amino acids has been confirmed in part by determination of the sequence of the first 40 amino acids from the NH2 terminus of the protein, and it shows 100% homology with the known amino acid sequence of the spinach protein but no more than 35% homology with the amino acid sequences of bacterial and mitochondrial proteins. The gene shows no deviation from the “universal” genetic code and is not split. A potential ribosome binding site is located 12 nucleotides upstream from the initiation codon, but sequences homologous to prokaryotic promotors and transcription terminators are not apparent.

Keywords: chloroplast DNA, dicyclohexylcarbodiimide-binding proteolipid, in vitro transcription-translation

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bhown A. S., Bennett J. C., Morgan P. H., Mole J. E. Use of fluorescamine as an effective blocking reagent to reduce the background in protein sequence analyses by the Beckman automated sequencer. Anal Biochem. 1981 Mar 15;112(1):158–162. doi: 10.1016/0003-2697(81)90274-8. [DOI] [PubMed] [Google Scholar]
  2. Borst P., Grivell L. A. Small is beautiful--portrait of a mitochondrial genome. Nature. 1981 Apr 9;290(5806):443–444. doi: 10.1038/290443a0. [DOI] [PubMed] [Google Scholar]
  3. Bottomley W., Whitfeld P. R. Cell-free transcription and translation of total spinach chloroplast DNA. Eur J Biochem. 1979 Jan 2;93(1):31–39. doi: 10.1111/j.1432-1033.1979.tb12791.x. [DOI] [PubMed] [Google Scholar]
  4. Brauer A. W., Margolies M. N., Haber E. The application of 0.1 M quadrol to the microsequence of proteins and the sequence of tryptic peptides. Biochemistry. 1975 Jul;14(13):3029–3035. doi: 10.1021/bi00684a036. [DOI] [PubMed] [Google Scholar]
  5. Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Doherty A., Gray J. C. Synthesis of a dicyclohexylcarbodiimide-binding proteolipid by isolated pea chloroplasts. Eur J Biochem. 1980;108(1):131–136. doi: 10.1111/j.1432-1033.1980.tb04703.x. [DOI] [PubMed] [Google Scholar]
  7. Downie J. A., Senior A. E., Gibson F., Cox G. B. A fifth gene (uncE) in the operon concerned with oxidative phosphorylation in Escherichia coli. J Bacteriol. 1979 Feb;137(2):711–718. doi: 10.1128/jb.137.2.711-718.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dretzen G., Bellard M., Sassone-Corsi P., Chambon P. A reliable method for the recovery of DNA fragments from agarose and acrylamide gels. Anal Biochem. 1981 Apr;112(2):295–298. doi: 10.1016/0003-2697(81)90296-7. [DOI] [PubMed] [Google Scholar]
  9. Friedl P., Bienhaus G., Hoppe J., Schairer H. U. The dicyclohexylcarbodiimide-binding protein c of ATP synthase from Escherichia coli is not sufficient to express an efficient H+ conduction. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6643–6646. doi: 10.1073/pnas.78.11.6643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gatenby A. A., Castleton J. A., Saul M. W. Expression in E. coli of maize and wheat chloroplast genes for large subunit of ribulose bisphosphate carboxylase. Nature. 1981 May 14;291(5811):117–121. doi: 10.1038/291117a0. [DOI] [PubMed] [Google Scholar]
  11. Gay N. J., Walker J. E. The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase. Nucleic Acids Res. 1981 Aug 25;9(16):3919–3926. doi: 10.1093/nar/9.16.3919. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gibson F., Downie J. A., Cox G. B., Radik J. Mu-induced polarity in the unc operon of Escherichia coli. J Bacteriol. 1978 Jun;134(3):728–736. doi: 10.1128/jb.134.3.728-736.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grebanier A. E., Coen D. M., Rich A., Bogorad L. Membrane proteins synthesized but not processed by isolated maize chloroplasts. J Cell Biol. 1978 Sep;78(3):734–746. doi: 10.1083/jcb.78.3.734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Hensgens L. A., Grivell L. A., Borst P., Bos J. L. Nucleotide sequence of the mitochondrial structural gene for subunit 9 of yeast ATPase complex. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1663–1667. doi: 10.1073/pnas.76.4.1663. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kanazawa H., Mabuchi K., Kayano T., Tamura F., Futai M. Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli. Biochem Biophys Res Commun. 1981 May 15;100(1):219–225. doi: 10.1016/s0006-291x(81)80085-x. [DOI] [PubMed] [Google Scholar]
  16. Nelson N., Eytan E., Notsani B. E., Sigrist H., Sigrist-Nelson K., Gitler C. Isolation of a chloroplast N,N'-dicyclohexylcarbodiimide-binding proteolipid, active in proton translocation. Proc Natl Acad Sci U S A. 1977 Jun;74(6):2375–2378. doi: 10.1073/pnas.74.6.2375. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Palmer J. D., Thompson W. F. Rearrangements in the chloroplast genomes of mung bean and pea. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5533–5537. doi: 10.1073/pnas.78.9.5533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reisfeld A., Mattoo A. K., Edelman M. Processing of a chloroplast-translated membrane protein in vivo. Analysis of the rapidly synthesized 32 000-dalton shield protein and its precursor in Spirodela oligorrhiza. Eur J Biochem. 1982 May;124(1):125–129. doi: 10.1111/j.1432-1033.1982.tb05914.x. [DOI] [PubMed] [Google Scholar]
  19. Rosenberg M., Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. doi: 10.1146/annurev.ge.13.120179.001535. [DOI] [PubMed] [Google Scholar]
  20. Sanger F., Coulson A. R., Barrell B. G., Smith A. J., Roe B. A. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. J Mol Biol. 1980 Oct 25;143(2):161–178. doi: 10.1016/0022-2836(80)90196-5. [DOI] [PubMed] [Google Scholar]
  21. Sebald W., Graf T., Lukins H. B. The dicyclohexylcarbodiimide-binding protein of the mitochondrial ATPase complex from Neurospora crassa and Saccharomyces cerevisiae. Identification and isolation. Eur J Biochem. 1979 Feb 1;93(3):587–599. doi: 10.1111/j.1432-1033.1979.tb12859.x. [DOI] [PubMed] [Google Scholar]
  22. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sierra M. F., Tzagoloff A. Assembly of the mitochondrial system. Purification of a mitochondrial product of the ATPase. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3155–3159. doi: 10.1073/pnas.70.11.3155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Tarr G. E., Beecher J. F., Bell M., McKean D. J. Polyquarternary amines prevent peptide loss from sequenators. Anal Biochem. 1978 Feb;84(2):622–7?0=ENG. doi: 10.1016/0003-2697(78)90086-6. [DOI] [PubMed] [Google Scholar]
  25. Tohdoh N., Shinozaki K., Sugiura M. Sequence of a putative promoter region for the rRNA genes of tobacco chloroplast DNA. Nucleic Acids Res. 1981 Oct 24;9(20):5399–5406. doi: 10.1093/nar/9.20.5399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tohdoh N., Sugiura M. The complete nucleotide sequence of 16S ribosomal RNA gene from tobacco chloroplasts. Gene. 1982 Feb;17(2):213–218. doi: 10.1016/0378-1119(82)90074-9. [DOI] [PubMed] [Google Scholar]
  27. Zimmerman C. L., Appella E., Pisano J. J. Rapid analysis of amino acid phenylthiohydantoins by high-performance liquid chromatography. Anal Biochem. 1977 Feb;77(2):569–573. doi: 10.1016/0003-2697(77)90276-7. [DOI] [PubMed] [Google Scholar]
  28. Zurawski G., Perrot B., Bottomley W., Whitfeld P. R. The structure of the gene for the large subunit of ribulose 1,5-bisphosphate carboxylase from spinach chloroplast DNA. Nucleic Acids Res. 1981 Jul 24;9(14):3251–3270. doi: 10.1093/nar/9.14.3251. [DOI] [PMC free article] [PubMed] [Google Scholar]

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