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. 1982 Dec;79(23):7253–7257. doi: 10.1073/pnas.79.23.7253

Deletion mutants defining the Escherichia coli replication factor Y effector site sequences in pBR322 DNA.

W C Soeller, K J Marians
PMCID: PMC347317  PMID: 6130524

Abstract

The Escherichia coli DNA replication factor Y, along with other genetically undefined replication proteins, is involved in a dnaB-, dnaC-, and dnaG-dependent pathway of primer formation on phi X174 single-stranded circular DNA. In addition, replication factor Y has a site-specific, single-stranded DNA-dependent ATPase activity. We have previously demonstrated the presence of two factor Y effector sites on pBR322 DNA. When inserted into the filamentous phage f1R229, these sites can function as rifampicin-resistant dnaB-, dnaC-, and dnaG-dependent origins of DNA replication. We report here the construction of deletion mutants of the two pBR322 factor Y effector sites. These deleted sites no longer function as effectors for factor Y ATPase activity nor as templates for rifampicin-resistant dnaB-, dnaC-, and dnaG-dependent DNA synthesis. We conclude that the DNA sequences required for factor Y ATPase activity and origin function are likely to be identical.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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