Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Dec;79(23):7326–7330. doi: 10.1073/pnas.79.23.7326

In vivo uptake of chylomicron [3H]retinyl ester by rat liver: evidence for retinol transfer from parenchymal to nonparenchymal cells.

R Blomhoff, P Helgerud, M Rasmussen, T Berg, K R Norum
PMCID: PMC347332  PMID: 6961410

Abstract

We have studied hepatic uptake of chylomicron retinyl ester. Chylomicrons were obtained from intestinal lymph of rats that were given retinol in groundnut oil by intraduodenal injection. When lymph was injected intravenously into normal rats, the radioactivity was cleared from blood with t1/2 approximately equal to 10 min. Retinyl ester was taken up initially by the liver, which, after 30 min, contained 80-90% of the radioactivity injected. Initially, most of the radioactivity was in hepatocytes, but after 30 min it disappeared from these cells and reappeared in nonparenchymal liver cells. After 2 hr these cells contained more radioactivity than the hepatocytes. When lymph was injected into vitamin A-deficient rats or rats given vitamin A in the form of retinoic acid, the plasma clearance and initial hepatic uptake of radioactivity were similar to that found in control animals. However, the nonparenchymal cells in these animals did not accumulate radioactivity. The current data suggest that vitamin A (in chylomicron remnants) is taken up initially by hepatocytes and then is released from these cells and delivered mainly to nonparenchymal liver cells in normal animals. In vitamin A-deficient rats, the vitamin is transferred from the hepatocytes to extrahepatic tissues.

Full text

PDF
7326

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bayfield R. F., Cole E. R. Colorimetric estimation of vitamin A with trichloroacetic acid. Methods Enzymol. 1980;67:189–195. doi: 10.1016/s0076-6879(80)67026-8. [DOI] [PubMed] [Google Scholar]
  2. Berg T., Boman D. Distribution of lysosomal enzymes between parenchymal and Kupffer cells of rat liver. Biochim Biophys Acta. 1973 Oct 10;321(2):585–596. doi: 10.1016/0005-2744(73)90201-5. [DOI] [PubMed] [Google Scholar]
  3. Bronfenmajer S., Schaffner F., Popper H. Fat-storing cells (lipocytes) in human liver. Arch Pathol. 1966 Nov;82(5):447–453. [PubMed] [Google Scholar]
  4. Brown M. S., Kovanen P. T., Goldstein J. L. Regulation of plasma cholesterol by lipoprotein receptors. Science. 1981 May 8;212(4495):628–635. doi: 10.1126/science.6261329. [DOI] [PubMed] [Google Scholar]
  5. Bucolo G., David H. Quantitative determination of serum triglycerides by the use of enzymes. Clin Chem. 1973 May;19(5):476–482. [PubMed] [Google Scholar]
  6. Drevon C. A. Cholesteryl ester metabolism in fat- and cholesterol/fat-fed guinea pigs. Atherosclerosis. 1978 Jun;30(2):123–136. doi: 10.1016/0021-9150(78)90055-2. [DOI] [PubMed] [Google Scholar]
  7. Drevon C. A., Norum K. R. Cholesterol esterification and lipids in plasma and liver from newborn and young guinea pigs raised on milk and non-milk diet. Nutr Metab. 1975;18(3):137–151. doi: 10.1159/000175588. [DOI] [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. GOODMAN D. W., HUANG H. S., SHIRATORI T. TISSUE DISTRIBUTION AND METABOLISM OF NEWLY ABSORBED VITAMIN A IN THE RAT. J Lipid Res. 1965 Jul;6:390–396. [PubMed] [Google Scholar]
  10. Goodman D. S. Vitamin A metabolism. Fed Proc. 1980 Aug;39(10):2716–2722. [PubMed] [Google Scholar]
  11. HAVEL R. J., EDER H. A., BRAGDON J. H. The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum. J Clin Invest. 1955 Sep;34(9):1345–1353. doi: 10.1172/JCI103182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Helgerud P., Petersen L. B., Norum K. R. Acyl CoA:retinol acyltransferase in rat small intestine: its activity and some properties of the enzymic reaction. J Lipid Res. 1982 May;23(4):609–618. [PubMed] [Google Scholar]
  13. Hirosawa K., Yamada E. The localization of the vitamin A in the mouse liver as revealed by electron microscope radioautography. J Electron Microsc (Tokyo) 1973;22(4):337–346. [PubMed] [Google Scholar]
  14. Hori S. H., Kitamura T. The vitamin A content and retinol esterifying activity of a Kupffer cell fraction of rat liver. J Histochem Cytochem. 1972 Oct;20(10):811–816. doi: 10.1177/20.10.811. [DOI] [PubMed] [Google Scholar]
  15. Hruban Z., Russell R. M., Boyer J. L., Glagov S., Bagheri S. A. Ultrastructural changes in livers of two patients with hypervitaminosis A. Am J Pathol. 1974 Sep;76(3):451–461. [PMC free article] [PubMed] [Google Scholar]
  16. Knook D. L., Sleyster E. C. Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriation. Exp Cell Res. 1976 May;99(2):444–449. doi: 10.1016/0014-4827(76)90605-4. [DOI] [PubMed] [Google Scholar]
  17. Lawrence C. W., Crain F. D., Lotspeich F. J., Krause R. F. Absorption, transport, and storage of retinyl-15-14C palmitate-9,10-3H in the rat. J Lipid Res. 1966 Mar;7(2):226–229. [PubMed] [Google Scholar]
  18. Lewis K. C., Green M. H., Underwood B. A. Vitamin A turnover in rats as influenced by vitamin A status. J Nutr. 1981 Jul;111(7):1135–1144. doi: 10.1093/jn/111.7.1135. [DOI] [PubMed] [Google Scholar]
  19. Linder M. C., Anderson G. H., Ascarelli I. Quantitative distribution of vitamin A in Kupffer cell and hepatocyte populations of rat liver. J Biol Chem. 1971 Sep 10;246(17):5538–5540. [PubMed] [Google Scholar]
  20. Lippiello P. M., Dijkstra J., van Galen M., Scherphof G., Waite B. M. The uptake and metabolism of chylomicron-remnant lipids by nonparenchymal cells in perfused liver and by Kupffer cells in culture. J Biol Chem. 1981 Jul 25;256(14):7454–7460. [PubMed] [Google Scholar]
  21. Munthe-Kaas A. C., Berg T., Seljelid R. Distribution of lysosomal enzymes in different types of rat liver cells. Exp Cell Res. 1976 Apr;99(1):146–154. doi: 10.1016/0014-4827(76)90689-3. [DOI] [PubMed] [Google Scholar]
  22. Munthe-Kaas A. C., Kaplan G., Seljelid R. On the mechanism of internalization of opsonized particles by rat Kupffer cells in vitro. Exp Cell Res. 1976 Nov;103(1):201–212. doi: 10.1016/0014-4827(76)90256-1. [DOI] [PubMed] [Google Scholar]
  23. Nilsson M., Berg T. Uptake and degradation of formaldehyde-treated 125I-labelled human serum albumin in rat liver cells in vivo and in vitro. Biochim Biophys Acta. 1977 Mar 29;497(1):171–182. doi: 10.1016/0304-4165(77)90150-7. [DOI] [PubMed] [Google Scholar]
  24. Nilsson M., Berg T. Uptake and degradation of formaldehyde-treated 125I-labelled human serum albumin in rat liver cells in vivo and in vitro. Biochim Biophys Acta. 1977 Mar 29;497(1):171–182. doi: 10.1016/0304-4165(77)90150-7. [DOI] [PubMed] [Google Scholar]
  25. Ose L., Ose T., Reinertsen R., Berg T. Fluid endocytosis in isolated rat parenchymal and non-parenchymal liver cells. Exp Cell Res. 1980 Mar;126(1):109–119. doi: 10.1016/0014-4827(80)90475-9. [DOI] [PubMed] [Google Scholar]
  26. Peterson P. A., Rask L., Ostberg L., Andersson L., Kamwendo F., Pertoft H. Studies on the transport and cellular distribution of vitamin A in normal and vitamin A-deficient rats with special reference to the vitamin A-binding plasma protein. J Biol Chem. 1973 Jun 10;248(11):4009–4022. [PubMed] [Google Scholar]
  27. RAO K. S., SASTRY P. S., GANGULY J. Fatty acid component of vitamin A ester in sheep liver. Arch Biochem Biophys. 1961 Nov;95:285–289. doi: 10.1016/0003-9861(61)90147-3. [DOI] [PubMed] [Google Scholar]
  28. Redgrave T. G., Vakakis N. Hepatic vitamin A fat-storage cells and the metabolism of chylomicron cholesterol. Aust J Exp Biol Med Sci. 1976 Dec;54(6):519–525. doi: 10.1038/icb.1976.53. [DOI] [PubMed] [Google Scholar]
  29. Ross A. C., Zilversmit D. B. Chylomicron remnant cholesteryl esters as the major constituent of very low density lipoproteins in plasma of cholesterol-fed rabbits. J Lipid Res. 1977 Mar;18(2):169–181. [PubMed] [Google Scholar]
  30. Tolleshaug H., Berg T., Nilsson M., Norum K. R. Uptake and degradation of 125I-labelled asialo-fetuin by isolated rat hepatocytes. Biochim Biophys Acta. 1977 Aug 25;499(1):73–84. doi: 10.1016/0304-4165(77)90230-6. [DOI] [PubMed] [Google Scholar]
  31. Wake K. Perisinusoidal stellate cells (fat-storing cells, interstitial cells, lipocytes), their related structure in and around the liver sinusoids, and vitamin A-storing cells in extrahepatic organs. Int Rev Cytol. 1980;66:303–353. doi: 10.1016/s0074-7696(08)61977-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES