Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Dec;79(24):7783–7787. doi: 10.1073/pnas.79.24.7783

Serum and epidermal growth factor transiently depolarize quiescent BSC-1 epithelial cells.

P Rothenberg, L Reuss, L Glaser
PMCID: PMC347432  PMID: 6984193

Abstract

Continuous intracellular recording of membrane potential with microelectrodes in BSC-1 epithelial cells has been used to study the early ionic effects of the interaction of mitogens with cell surface receptors. Initial results show that (i) there is no significant difference in membrane potential between growing and quiescent cells, (ii) addition of epidermal growth factor or serum to quiescent BSC-1 cells induces a brief and transient depolarization, (iii) the mitogenic response of BSC-1 cells to epidermal growth factor and the transient depolarization show similar concentration dependences, and (iv) serum addition to quiescent BSC-1 cells induces a sustained increase in Na+ influx that is electroneutral and amiloride sensitive. Intracellular pH changes may be a primary event triggering the response of quiescent cells to mitogenic polypeptides.

Full text

PDF
7783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Averdunk R., Lauf P. K. Effects of mitogens on sodium-potassium transport, 3H-ouabain binding, and adenosine triphosphatase activity in lymphocytes. Exp Cell Res. 1975 Jul;93(2):331–342. doi: 10.1016/0014-4827(75)90458-9. [DOI] [PubMed] [Google Scholar]
  2. Benos D. J. Amiloride: a molecular probe of sodium transport in tissues and cells. Am J Physiol. 1982 Mar;242(3):C131–C145. doi: 10.1152/ajpcell.1982.242.3.C131. [DOI] [PubMed] [Google Scholar]
  3. Boonstra J., Mummery C. L., Tertoolen L. G., Van Der Saag P. T., De Laat S. W. Cation transport and growth regulation in neuroblastoma cells. Modulations of K+ transport and electrical membrane properties during the cell cycle. J Cell Physiol. 1981 Apr;107(1):75–83. doi: 10.1002/jcp.1041070110. [DOI] [PubMed] [Google Scholar]
  4. Carpenter G., King L., Jr, Cohen S. Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. J Biol Chem. 1979 Jun 10;254(11):4884–4891. [PubMed] [Google Scholar]
  5. Davis M. H., Pato C. N., Gruenstein E. Analysis of neurotoxin and mitogen-stimulated sodium transport in human fibroblasts. J Biol Chem. 1982 Apr 25;257(8):4356–4361. [PubMed] [Google Scholar]
  6. Epel D. Mechanisms of activation of sperm and egg during fertilization of sea urchin gametes. Curr Top Dev Biol. 1978;12:185–246. doi: 10.1016/s0070-2153(08)60597-9. [DOI] [PubMed] [Google Scholar]
  7. Fehlmann M., Samson M., Koch K. S., Leffert H. L., Freychet P. Amiloride inhibits protein synthesis in isolated rat hepatocytes. Life Sci. 1981 Mar 16;28(11):1295–1302. doi: 10.1016/0024-3205(81)90456-2. [DOI] [PubMed] [Google Scholar]
  8. Freedman M. H. Early biochemical events in lymphocyte activation. I. Investigations on the nature and significance of early calcium fluxes observed in mitogen-induced T and B lymphocytes. Cell Immunol. 1979 May;44(2):290–313. doi: 10.1016/0008-8749(79)90007-8. [DOI] [PubMed] [Google Scholar]
  9. HOPPS H. E., BERNHEIM B. C., NISALAK A., TJIO J. H., SMADEL J. E. BIOLOGIC CHARACTERISTICS OF A CONTINUOUS KIDNEY CELL LINE DERIVED FROM THE AFRICAN GREEN MONKEY. J Immunol. 1963 Sep;91:416–424. [PubMed] [Google Scholar]
  10. Heine U. I., Keski-Oja J., Wetzel B. Rapid membrane changes in mouse epithelial cells after exposure to epidermal growth factor. J Ultrastruct Res. 1981 Dec;77(3):335–343. doi: 10.1016/s0022-5320(81)80029-9. [DOI] [PubMed] [Google Scholar]
  11. Holley R. W., Armour R., Baldwin J. H., Brown K. D., Yeh Y. C. Density-dependent regulation of growth of BSC-1 cells in cell culture: control of growth by serum factors. Proc Natl Acad Sci U S A. 1977 Nov;74(11):5046–5050. doi: 10.1073/pnas.74.11.5046. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hunter T., Cooper J. A. Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells. Cell. 1981 Jun;24(3):741–752. doi: 10.1016/0092-8674(81)90100-8. [DOI] [PubMed] [Google Scholar]
  13. Jan Y. N., Jan L. Y., Kuffler S. W. Further evidence for peptidergic transmission in sympathetic ganglia. Proc Natl Acad Sci U S A. 1980 Aug;77(8):5008–5012. doi: 10.1073/pnas.77.8.5008. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Johnson J. D., Epel D. Intracellular pH and activation of sea urchin eggs after fertilisation. Nature. 1976 Aug 19;262(5570):661–664. doi: 10.1038/262661a0. [DOI] [PubMed] [Google Scholar]
  15. Koch K. S., Leffert H. L. Increased sodium ion influx is necessary to initiate rat hepatocyte proliferation. Cell. 1979 Sep;18(1):153–163. doi: 10.1016/0092-8674(79)90364-7. [DOI] [PubMed] [Google Scholar]
  16. Moolenaar W. H., Mummery C. L., van der Saag P. T., de Laat S. W. Rapid ionic events and the initiation of growth in serum-stimulated neuroblastoma cells. Cell. 1981 Mar;23(3):789–798. doi: 10.1016/0092-8674(81)90443-8. [DOI] [PubMed] [Google Scholar]
  17. Moolenaar W. H., Yarden Y., de Laat S. W., Schlessinger J. Epidermal growth factor induces electrically silent Na+ influx in human fibroblasts. J Biol Chem. 1982 Jul 25;257(14):8502–8506. [PubMed] [Google Scholar]
  18. Nicoll R. A. The action of thyrotropin-releasing hormone, substance P and related peptides on frog spinal motoneurons. J Pharmacol Exp Ther. 1978 Dec;207(3):817–824. [PubMed] [Google Scholar]
  19. Pardee A. B., Dubrow R., Hamlin J. L., Kletzien R. F. Animal cell cycle. Annu Rev Biochem. 1978;47:715–750. doi: 10.1146/annurev.bi.47.070178.003435. [DOI] [PubMed] [Google Scholar]
  20. Pouysségur J., Chambard J. C., Franchi A., Paris S., Van Obberghen-Schilling E. Growth factor activation of an amiloride-sensitive Na+/H+ exchange system in quiescent fibroblasts: coupling to ribosomal protein S6 phosphorylation. Proc Natl Acad Sci U S A. 1982 Jul;79(13):3935–3939. doi: 10.1073/pnas.79.13.3935. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Roos A., Boron W. F. Intracellular pH. Physiol Rev. 1981 Apr;61(2):296–434. doi: 10.1152/physrev.1981.61.2.296. [DOI] [PubMed] [Google Scholar]
  22. Rozengurt E., Heppel L. A. Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4492–4495. doi: 10.1073/pnas.72.11.4492. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rozengurt E., Mendoza S. Monovalent ion fluxes and the control of cell proliferation in cultured fibroblasts. Ann N Y Acad Sci. 1980;339:175–190. doi: 10.1111/j.1749-6632.1980.tb15977.x. [DOI] [PubMed] [Google Scholar]
  24. Savage C. R., Jr, Cohen S. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization. J Biol Chem. 1972 Dec 10;247(23):7609–7611. [PubMed] [Google Scholar]
  25. Sawyer S. T., Cohen S. Enhancement of calcium uptake and phosphatidylinositol turnover by epidermal growth factor in A-431 cells. Biochemistry. 1981 Oct 13;20(21):6280–6286. doi: 10.1021/bi00524a057. [DOI] [PubMed] [Google Scholar]
  26. Shen S. S., Steinhardt R. A. Direct measurement of intracellular pH during metabolic derepression of the sea urchin egg. Nature. 1978 Mar 16;272(5650):253–254. doi: 10.1038/272253a0. [DOI] [PubMed] [Google Scholar]
  27. Smith J. B., Rozengurt E. Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5560–5564. doi: 10.1073/pnas.75.11.5560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Smith R. L., Macara I. G., Levenson R., Housman D., Cantley L. Evidence that a Na+/Ca2+ antiport system regulates murine erythroleukemia cell differentiation. J Biol Chem. 1982 Jan 25;257(2):773–780. [PubMed] [Google Scholar]
  29. Taub M. Isolation of amiloride-resistant clones from dog kidney epithelial cells. Somatic Cell Genet. 1978 Sep;4(5):609–616. doi: 10.1007/BF01542930. [DOI] [PubMed] [Google Scholar]
  30. Villereal M. L., Cook J. S. Regulation of active amino acid transport by growth-related changes in membrane potential in a human fibroblast. J Biol Chem. 1978 Nov 25;253(22):8257–8262. [PubMed] [Google Scholar]
  31. Villereal M. L. Sodium fluxes in human fibroblasts: effect of serum, Ca+2, and amiloride. J Cell Physiol. 1981 Jun;107(3):359–369. doi: 10.1002/jcp.1041070307. [DOI] [PubMed] [Google Scholar]
  32. Villereal M. L. Sodium fluxes in human fibroblasts: kinetics of serum-dependent and serum-independent pathways. J Cell Physiol. 1981 Aug;108(2):251–259. doi: 10.1002/jcp.1041080215. [DOI] [PubMed] [Google Scholar]
  33. Whittenberger B., Raben D., Lieberman M. A., Glaser L. Inhibition of growth of 3T3 cells by extract of surface membranes. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5457–5461. doi: 10.1073/pnas.75.11.5457. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES