Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1982 Dec;79(24):7809–7813. doi: 10.1073/pnas.79.24.7809

Chromosomal localization of human leukocyte, fibroblast, and immune interferon genes by means of in situ hybridization

Jeffrey M Trent *, Sharon Olson *, Richard M Lawn †
PMCID: PMC347438  PMID: 6818550

Abstract

Recombinant plasmids containing cDNA inserts from human leukocyte interferon (IFN-α), fibroblast interferon (IFN-β), or immune interferon (INF-γ) genes were radiolabeled and hybridized in situ to human metaphase chromosome preparations. The results localized the human IFN-α and IFN-β genes to the short arm of chromosome 9(p21→pter) and localized the IFN-γ gene to the long arm of chromosome 12(q24.1).

Keywords: human metaphase chromosomes

Full text

PDF
7809

Images in this article

7810Image
on p.7810
7811Image
on p.7811
7811Image
on p.7811
7812Image
on p.7812

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brack C., Nagata S., Mantei N., Weissmann C. Molecular analysis of the human interferon-alpha gene family. Gene. 1981 Dec;15(4):379–394. doi: 10.1016/0378-1119(81)90181-5. [DOI] [PubMed] [Google Scholar]
  2. Chandler M. E., Kedes L. H., Cohn R. H., Yunis J. J. Genes coding for histone proteins in man are located on the distal end of the long arm of chromosome 7. Science. 1979 Aug 31;205(4409):908–910. doi: 10.1126/science.472711. [DOI] [PubMed] [Google Scholar]
  3. Chandler M. E., Yunis J. J. A high resolution in situ hybridization technique for the direct visualization of labeled G-banded early metaphase and prophase chromosomes. Cytogenet Cell Genet. 1978;22(1-6):352–356. doi: 10.1159/000130970. [DOI] [PubMed] [Google Scholar]
  4. Derynck R., Content J., DeClercq E., Volckaert G., Tavernier J., Devos R., Fiers W. Isolation and structure of a human fibroblast interferon gene. Nature. 1980 Jun 19;285(5766):542–547. doi: 10.1038/285542a0. [DOI] [PubMed] [Google Scholar]
  5. Durie B. G., Salmon S. E. High speed scintillation autoradiography. Science. 1975 Dec 12;190(4219):1093–1095. doi: 10.1126/science.1188385. [DOI] [PubMed] [Google Scholar]
  6. Gerhard D. S., Kawasaki E. S., Bancroft F. C., Szabo P. Localization of a unique gene by direct hybridization in situ. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3755–3759. doi: 10.1073/pnas.78.6.3755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Goeddel D. V., Leung D. W., Dull T. J., Gross M., Lawn R. M., McCandliss R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. The structure of eight distinct cloned human leukocyte interferon cDNAs. Nature. 1981 Mar 5;290(5801):20–26. doi: 10.1038/290020a0. [DOI] [PubMed] [Google Scholar]
  8. Goeddel D. V., Shepard H. M., Yelverton E., Leung D., Crea R., Sloma A., Pestka S. Synthesis of human fibroblast interferon by E. coli. Nucleic Acids Res. 1980 Sep 25;8(18):4057–4074. doi: 10.1093/nar/8.18.4057. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goeddel D. V., Yelverton E., Ullrich A., Heyneker H. L., Miozzari G., Holmes W., Seeburg P. H., Dull T., May L., Stebbing N. Human leukocyte interferon produced by E. coli is biologically active. Nature. 1980 Oct 2;287(5781):411–416. doi: 10.1038/287411a0. [DOI] [PubMed] [Google Scholar]
  10. Gray P. W., Leung D. W., Pennica D., Yelverton E., Najarian R., Simonsen C. C., Derynck R., Sherwood P. J., Wallace D. M., Berger S. L. Expression of human immune interferon cDNA in E. coli and monkey cells. Nature. 1982 Feb 11;295(5849):503–508. doi: 10.1038/295503a0. [DOI] [PubMed] [Google Scholar]
  11. Gusella J. F., Keys C., VarsanyiBreiner A., Kao F. T., Jones C., Puck T. T., Housman D. Isolation and localization of DNA segments from specific human chromosomes. Proc Natl Acad Sci U S A. 1980 May;77(5):2829–2833. doi: 10.1073/pnas.77.5.2829. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Harper M. E., Barrera-Saldaña H. A., Saunders G. F. Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24. Am J Hum Genet. 1982 Mar;34(2):227–234. [PMC free article] [PubMed] [Google Scholar]
  13. Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
  14. Lawn R. M., Adelman J., Dull T. J., Gross M., Goeddel D., Ullrich A. DNA sequence of two closely linked human leukocyte interferon genes. Science. 1981 Jun 5;212(4499):1159–1162. doi: 10.1126/science.6165082. [DOI] [PubMed] [Google Scholar]
  15. Meager A., Graves H. E., Walker J. R., Burke D. C., Swallow D. M., Westerveld A. Somatic cell genetics of human interferon production in human-rodent cell hybrids. J Gen Virol. 1979 Nov;45(2):309–321. doi: 10.1099/0022-1317-45-2-309. [DOI] [PubMed] [Google Scholar]
  16. Nagata S., Brack C., Henco K., Schamböck A., Weissmann C. Partial mapping of ten genes of the human interferon- alpha family. J Interferon Res. 1981 Feb;1(2):333–336. doi: 10.1089/jir.1981.1.333. [DOI] [PubMed] [Google Scholar]
  17. Nagata S., Mantei N., Weissmann C. The structure of one of the eight or more distinct chromosomal genes for human interferon-alpha. Nature. 1980 Oct 2;287(5781):401–408. doi: 10.1038/287401a0. [DOI] [PubMed] [Google Scholar]
  18. Nagata S., Taira H., Hall A., Johnsrud L., Streuli M., Ecsödi J., Boll W., Cantell K., Weissmann C. Synthesis in E. coli of a polypeptide with human leukocyte interferon activity. Nature. 1980 Mar 27;284(5754):316–320. doi: 10.1038/284316a0. [DOI] [PubMed] [Google Scholar]
  19. Owerbach D., Bell G. I., Rutter W. J., Shows T. B. The insulin gene is located on chromosome 11 in humans. Nature. 1980 Jul 3;286(5768):82–84. doi: 10.1038/286082a0. [DOI] [PubMed] [Google Scholar]
  20. Owerbach D., Rutter W. J., Martial J. A., Baxter J. D., Shows T. B. Genes for growth hormone, chorionic somatommammotropin, and growth hormones-like gene on chromosome 17 in humans. Science. 1980 Jul 11;209(4453):289–292. doi: 10.1126/science.7384802. [DOI] [PubMed] [Google Scholar]
  21. Owerbach D., Rutter W. J., Roberts J. L., Whitfeld P., Shine J., Seeburg P. H., Shows T. B. The proopiocortin (adrenocorticotropin/beta-lipoprotein) gene is located on chromosome 2 in humans. Somatic Cell Genet. 1981 May;7(3):359–369. doi: 10.1007/BF01538860. [DOI] [PubMed] [Google Scholar]
  22. Owerbach D., Rutter W. J., Shows T. B., Gray P., Goeddel D. V., Lawn R. M. Leukocyte and fibroblast interferon genes are located on human chromosome 9. Proc Natl Acad Sci U S A. 1981 May;78(5):3123–3127. doi: 10.1073/pnas.78.5.3123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sehgal P. B., Sagar A. D. Heterogeneity of poly(I) x poly(C)-induced human fibroblast interferon mRNA species. Nature. 1980 Nov 6;288(5786):95–97. doi: 10.1038/288095a0. [DOI] [PubMed] [Google Scholar]
  24. Shows T. B., Sakaguchi A. Y., Naylor S. L., Goedell D. V., Lawn R. M. Clustering of leukocyte and fibroblast interferon genes of human chromosome 9. Science. 1982 Oct 22;218(4570):373–374. doi: 10.1126/science.6181564. [DOI] [PubMed] [Google Scholar]
  25. Slate D. L., Ruddle F. H. Fibroblast interferon in man is coded by two loci on separate chromosomes. Cell. 1979 Jan;16(1):171–180. doi: 10.1016/0092-8674(79)90198-3. [DOI] [PubMed] [Google Scholar]
  26. Tan Y. H., Berthold W. A mechanism for the induction and regulation of human fibroblastoid interferon genetic expression. J Gen Virol. 1977 Mar;34(3):401–411. doi: 10.1099/0022-1317-34-3-401. [DOI] [PubMed] [Google Scholar]
  27. Tan Y. H., Creagan R. P., Ruddle F. H. The somatic cell genetics of human interferon: assignment of human interferon loci to chromosomes 2 and 5. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2251–2255. doi: 10.1073/pnas.71.6.2251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Tan Y. H., Schneider E. L., Tischfield J., Epstein C. J., Ruddle F. H. Human chromosome 21 dosage: effect on the expression of the interferon induced antiviral state. Science. 1974 Oct 4;186(4158):61–63. doi: 10.1126/science.186.4158.61. [DOI] [PubMed] [Google Scholar]
  29. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  30. Ullrich A., Gray A., Goeddel D. V., Dull T. J. Nucleotide sequence of a portion of human chromosome 9 containing a leukocyte interferon gene cluster. J Mol Biol. 1982 Apr 15;156(3):467–486. doi: 10.1016/0022-2836(82)90261-3. [DOI] [PubMed] [Google Scholar]
  31. Wahl G. M., Stern M., Stark G. R. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683–3687. doi: 10.1073/pnas.76.8.3683. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Weissenbach J., Chernajovsky Y., Zeevi M., Shulman L., Soreq H., Nir U., Wallach D., Perricaudet M., Tiollais P., Revel M. Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies. Proc Natl Acad Sci U S A. 1980 Dec;77(12):7152–7156. doi: 10.1073/pnas.77.12.7152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yu L. C., Szabo P., Borun T. W., Prensky W. The localization of the genes coding for histone H4 in human chromosomes. Cold Spring Harb Symp Quant Biol. 1978;42(Pt 2):1101–1105. doi: 10.1101/sqb.1978.042.01.111. [DOI] [PubMed] [Google Scholar]
  34. Yunis J. J., Kuo M. T., Saunders G. F. Localization of sequences specifying messenger RNA to light-staining G-bands of human chromosomes. Chromosoma. 1977 Jun 23;61(4):335–344. doi: 10.1007/BF00288617. [DOI] [PubMed] [Google Scholar]
  35. Yunis J. J., Sawyer J. R., Ball D. W. Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping. Cytogenet Cell Genet. 1978;22(1-6):679–683. doi: 10.1159/000131052. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES