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. 1982 Dec;79(24):7847–7851. doi: 10.1073/pnas.79.24.7847

Anti-idiotypes against anti-H-2 monoclonal antibodies: structural analysis of the molecules induced by in vivo anti-idiotype treatment.

J A Bluestone, H C Krutzsch, H Auchincloss Jr, P A Cazenave, T J Kindt, D H Sachs
PMCID: PMC347446  PMID: 6818552

Abstract

Previous studies have shown that treatment of mice in vivo with xenogeneic anti-idiotype produced against a monoclonal anti-H-2Kk antibody, 11-4.1, leads to the induction of molecules (Id') that inhibit the binding of anti-idiotype to idiotype. To investigate the nature of these Id' molecules, spleens from such anti-idiotype-treated mice were fused with the SP2/0 myeloma to produce monoclonal Id' antibodies. All four monoclonal Id' antibodies were found to react with goat and rabbit anti-11-4.1 in addition to the pig anti-idiotype used for their induction and one of the four, J1-8-1, reacted with syngeneic BALB/c anti-11-4.1. Partial amino acid sequences were determined for the heavy and light chains of these monoclonal antibodies. J1-8-1 heavy chain had an NH2-terminal amino acid sequence identical to that of 11-4.1 for the 39 NH2-terminal residues assigned, whereas its light chain and the heavy and light chains of the other Id' molecules differed markedly from those of the 11-4.1 antibody. Isolated heavy chains and light chains of J1-8-1 and 11-4.1 were reassociated in homologous and heterologous pairs. When J1-8-1 heavy chains and 11-4.1 light chains were mixed in equimolar concentrations, anti-H-2Kk reactivity was found at a level approximately 10% of that observed for reassociated 11-4.1 homologous heavy and light chains. The finding that in vivo anti-idiotype treatment can trigger Id' molecules structurally similar to the original idiotype has implications regarding the mechanism of induction of Id' molecules and the regulation of repertoire expression by idiotypic networks.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bluestone J. A., Epstein S. L., Ozato K., Sharrow S. O., Sachs D. H. Anti-idiotypes to monoclonal anti-H-2 antibodies. II. Expression of anti-H-2Kk idiotypes on antibodies induced by anti-idiotype or H-2Kk antigen. J Exp Med. 1981 Nov 1;154(5):1305–1318. doi: 10.1084/jem.154.5.1305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bluestone J. A., Metzger J. J., Knode M. C., Ozato K., Sachs D. H. Anti-idiotypes to monoclonal anti-H-2 antibodies--I. Contribution of isolated heavy and light chains to idiotype expression. Mol Immunol. 1982 Apr;19(4):515–524. doi: 10.1016/0161-5890(82)90219-x. [DOI] [PubMed] [Google Scholar]
  3. Bluestone J. A., Sharrow S. O., Epstein S. L., Ozato K., Sachs D. H. Induction of anti-H-2 antibodies without alloantigen exposure by in vivo administration of anti-idiotype. Nature. 1981 May 21;291(5812):233–235. doi: 10.1038/291233a0. [DOI] [PubMed] [Google Scholar]
  4. Epstein S. L., Ozato K., Bluestone J. A., Sachs D. H. Idiotypes of anti-Ia antibodies. I. Expression of the 14-4-4S idiotype in humoral immune responses. J Exp Med. 1981 Aug 1;154(2):397–409. doi: 10.1084/jem.154.2.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gates F. T., 3rd, Coligan J. E., Kindt T. J. Complete amino acid sequence of rabbit beta 2-microglobulin. Biochemistry. 1979 May 29;18(11):2267–2272. doi: 10.1021/bi00578a021. [DOI] [PubMed] [Google Scholar]
  6. Nisonoff A., Lamoyi E. Implications of the presence of an internal image of the antigen in anti-idiotypic antibodies: possible application to vaccine production. Clin Immunol Immunopathol. 1981 Dec;21(3):397–406. doi: 10.1016/0090-1229(81)90228-2. [DOI] [PubMed] [Google Scholar]
  7. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  8. Ozato K., Mayer N., Sachs D. H. Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens. J Immunol. 1980 Feb;124(2):533–540. [PubMed] [Google Scholar]
  9. Podell D. N., Abraham G. N. A technique for the removal of pyroglutamic acid from the amino terminus of proteins using calf liver pyroglutamate amino peptidase. Biochem Biophys Res Commun. 1978 Mar 15;81(1):176–185. doi: 10.1016/0006-291x(78)91646-7. [DOI] [PubMed] [Google Scholar]
  10. Sachs D. H., El-Gamil M., Miller G. Genetic control of the immune response to staphylococcal nuclease. XI. Effects of in vivo administration of anti-idiotypic antibodies. Eur J Immunol. 1981 Jun;11(6):509–516. doi: 10.1002/eji.1830110613. [DOI] [PubMed] [Google Scholar]
  11. Sharrow S. O., Ozato K., Sachs D. H. Phenotypic expression of I-A and I-E/C subregion determinants on murine thymocytes. J Immunol. 1980 Nov;125(5):2263–2268. [PubMed] [Google Scholar]
  12. Winkler M., Franssen J. D., Collignon C., Leo O., Mariamé B., van de Walle P., de Groote D., Urbain J. Idiotypic regulation of the immune system. Common idiotypic specificities between idiotypes and antibodies raised against anti-idiotypic antibodies in rabbits. J Exp Med. 1979 Jul 1;150(1):184–195. doi: 10.1084/jem.150.1.184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wysocki L. J., Sato V. L. The strain A anti-p-azophenylarsonate major cross-reactive idiotypic family includes members with no reactivity toward p-azophenylarsonate. Eur J Immunol. 1981 Oct;11(10):832–839. doi: 10.1002/eji.1830111016. [DOI] [PubMed] [Google Scholar]

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