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. 1982 Dec;79(24):7866–7870. doi: 10.1073/pnas.79.24.7866

Human alveolar macrophages produce leukotriene B4.

A O Fels, N A Pawlowski, E B Cramer, T K King, Z A Cohn, W A Scott
PMCID: PMC347450  PMID: 6296853

Abstract

Human alveolar macrophages obtained by bronchoalveolar lavage were labeled overnight with [3H]arachidonic acid. The cells were stimulated with calcium ionophore A23187, and the 20:4 oxygenated metabolites released into the culture medium were identified by reverse-phase HPLC. Leukotriene B4 was the major 20:4 metabolite produced by these cultures. Leukotriene B4 was identified by its reverse-phase HPLC elution time, its UV spectrum, and its chemotactic and chemokinetic activities for neutrophils. In addition, the macrophage- and neutrophil-derived leukotriene B4 free acids and methyl esters were found to have identical HPLC retention times.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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