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. 1982 Jul;37(1):82–88. doi: 10.1128/iai.37.1.82-88.1982

Antibody response of infants to cell surface-exposed outer membrane proteins of Haemophilus influenzae type b after systemic Haemophilus disease.

P A Gulig, G H McCracken Jr, C F Frisch, K H Johnston, E J Hansen
PMCID: PMC347494  PMID: 6980838

Abstract

The immune response of nine infants with Haemophilus influenzae type b meningitis was examined by using a radioimmunoprecipitation procedure designed to detect antibodies directed against cell surface-exposed outer membrane proteins of this pathogen. Using intrinsically or extrinsically radiolabeled intact H. influenzae type b cells with acute- and convalescent-phase human sera in this radioimmunoprecipitation system, we found that all of the infants produced an antibody response directed against several different H. influenzae type b outer membrane proteins. Anti-H. influenzae type b outer membrane protein antibodies present in convalescent sera, but not found in acute sera, were directed against cell surface-exposed H. influenzae type b outer membrane proteins. In contrast, both acute and convalescent sera contained antibody activity directed against numerous H. influenzae type b outer membrane proteins whose antigenic determinants were apparently inaccessible to antibody on intact H. influenzae type b cells. The ability of infants to develop an antibody response to cell surface-exposed, antibody-accessible H. influenzae type b outer membrane proteins indicates that these proteins may have vaccinogenic potential.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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