Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Jul;37(1):172–178. doi: 10.1128/iai.37.1.172-178.1982

Production and partial characterization of monoclonal antibodies to Mycobacterium leprae.

T P Gillis, T M Buchanan
PMCID: PMC347507  PMID: 6179876

Abstract

Monoclonal antibodies to Mycobacterium leprae were produced by the fusion of BALB/c splenocytes and lymph node cells to BALB/c myeloma (NSI/1) cells. Eleven monoclonal antibodies were characterized as to their reactivity with M. leprae and 18 other mycobacterial species by enzyme-linked immunosorbent assay and immunofluorescence. Two monoclonal antibodies reacted only with M. leprae, and the other nine showed unique patterns of reactivity by enzyme-linked immunosorbent assay. One monoclonal antibody (IIH9) reacted with a 68,000-dalton protein present in extracts from M. leprae, M. tuberculosis H37Rv, M. gastri, and M. smegmatis. Potential uses for these antibodies in serological tests and immunochemical analyses are discussed.

Full text

PDF

Images in this article

176Image
on p.176

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe M., Minagawa F., Yoshino Y., Okamura K. Studies on the antigenic specificity of Mycobacterium leprae. II. Purification and immunological characterization of the soluble antigen in leprosy nodules. Int J Lepr Other Mycobact Dis. 1972 Apr-Jun;40(2):107–117. [PubMed] [Google Scholar]
  2. Abe M., Minagawa F., Yoshino Y., Ozawa T., Saikawa K., Saito T. Fluorescent leprosy antibody absorption (FLA-ABS) test for detecting subclinical infection with Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1980 Jun;48(2):109–119. [PubMed] [Google Scholar]
  3. Axelsen N. H., Harboe M., Closs O., Godal T. BCG antibody profiles in tuberculoid and lepromatous leprosy. Infect Immun. 1974 May;9(5):952–958. doi: 10.1128/iai.9.5.952-958.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brennan P. J., Barrow W. W. Evidence for species-specific lipid antigens in Mycobacterium leprae. Int J Lepr Other Mycobact Dis. 1980 Dec;48(4):382–387. [PubMed] [Google Scholar]
  5. Brennan P. J., Goren M. B. Structural studies on the type-specific antigens and lipids of the mycobacterium avium. Mycobacterium intracellulare. Mycobacterium scrofulaceum serocomplex. Mycobacterium intracellulare serotype 9. J Biol Chem. 1979 May 25;254(10):4205–4211. [PubMed] [Google Scholar]
  6. Estrada-Parra S. Immunochemical identification of a defined antigen of Mycobacterium leprae. Infect Immun. 1972 Feb;5(2):258–259. doi: 10.1128/iai.5.2.258-259.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gillis T. P., Abe M., Bullock W. E., Rojas-Espinosa O., Garcia-Ortigoza E., Draper P., Kirchheimer W., Buchanan T. M. Comparison of 22 species of Mycobacteria by immunodiffusion against an absorbed reference leprosy serum. Int J Lepr Other Mycobact Dis. 1981 Sep;49(3):287–293. [PubMed] [Google Scholar]
  8. Harboe M., Closs O., Bjune G., Kronvall G., Axelsen N. H. Mycobacterium leprae specific antibodies detected by radioimmunoassay. Scand J Immunol. 1978;7(2):111–120. doi: 10.1111/j.1365-3083.1978.tb00433.x. [DOI] [PubMed] [Google Scholar]
  9. Johnson G. D., Nogueira Araujo G. M. A simple method of reducing the fading of immunofluorescence during microscopy. J Immunol Methods. 1981;43(3):349–350. doi: 10.1016/0022-1759(81)90183-6. [DOI] [PubMed] [Google Scholar]
  10. Kirchheimer W. F., Storrs E. E. Attempts to establish the armadillo (Dasypus novemcinctus Linn.) as a model for the study of leprosy. I. Report of lepromatoid leprosy in an experimentally infected armadillo. Int J Lepr Other Mycobact Dis. 1971 Jul-Sep;39(3):693–702. [PubMed] [Google Scholar]
  11. Kwapinski J. B., Alcasid A., Kwapinski E. H., Nairn V. The immunology of cytoplasmic antigens of mycobacteria. Can J Microbiol. 1972 Aug;18(8):1201–1211. doi: 10.1139/m72-188. [DOI] [PubMed] [Google Scholar]
  12. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  13. Köhler G., Milstein C. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol. 1976 Jul;6(7):511–519. doi: 10.1002/eji.1830060713. [DOI] [PubMed] [Google Scholar]
  14. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  15. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  16. Lostrom M. E., Stone M. R., Tam M., Burnette W. N., Pinter A., Nowinski R. C. Monoclonal antibodies against murine leukemia viruses: identification of six antigenic determinants on the p 15(E) and gp70 envelope proteins. Virology. 1979 Oct 30;98(2):336–350. doi: 10.1016/0042-6822(79)90557-9. [DOI] [PubMed] [Google Scholar]
  17. Navalkar R. G. Immunologic studies on leprosy. 2. Antigenic studies of Mycobacterium leprae. Z Tropenmed Parasitol. 1973 Dec;24(Suppl):66–72. [PubMed] [Google Scholar]
  18. Navalkar R. G., Patel P. J., Dalvi R. R. Immunological studies on leprosy: separation and evaluation of the antigens of Mycobacterium leprae. J Med Microbiol. 1975 May;8(2):319–324. doi: 10.1099/00222615-8-2-319. [DOI] [PubMed] [Google Scholar]
  19. Renart J., Reiser J., Stark G. R. Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3116–3120. doi: 10.1073/pnas.76.7.3116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Stone M. R., Nowinski R. C. Topological mapping of murine leukemia virus proteins by competition-binding assays with monoclonal antibodies. Virology. 1980 Jan 30;100(2):370–381. doi: 10.1016/0042-6822(80)90528-0. [DOI] [PubMed] [Google Scholar]
  21. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES