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. 1982 Jul;37(1):243–249. doi: 10.1128/iai.37.1.243-249.1982

Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion.

B F Fernie, J L Gerin
PMCID: PMC347519  PMID: 7107006

Abstract

We identified by immunochemical methods 13 polypeptides associated with the infectious respiratory syncytial virus virion. Eight of these polypeptides (VP200, VP84, VP66, VP43, VP40, VP37, VP28, and VP19) were identified as virus specific. Two other polypeptides, (VP) 22 and (VP) 12, are provisionally considered to be of viral origin. Three nonviral proteins are also intimately associated with the infectious virion. These nonviral proteins were identified as cellular actin and two proteins with bovine serum albumin immunospecificity. VP40 was identified as the major ribonucleoprotein. Based on biochemical and biophysical similarities with paramyxovirus proteins, other respiratory syncytial virus proteins are believed to have these specific viral functions: VP84, "hemagglutinin"; VP66, undissociated fusion protein, F1,2; VP43, F1; and VP19, F2, VP66 contains a major determinant involved in viral infectivity since all neutralizing antibodies tested, including a monoclone, precipitated this protein.

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Selected References

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  1. Anker H. S. A solubilizable acrylamide gel for electrophoresis. FEBS Lett. 1970 Apr 16;7(3):293–293. doi: 10.1016/0014-5793(70)80185-5. [DOI] [PubMed] [Google Scholar]
  2. Belshe R. B., Richardson L. S., London W. T., Sly D. L., Lorfeld J. H., Camargo E., Prevar D. A., Chanock R. M. Experimental respiratory syncytial virus infection of four species of primates. J Med Virol. 1977;1(3):157–162. doi: 10.1002/jmv.1890010302. [DOI] [PubMed] [Google Scholar]
  3. Bernstein J. M., Hruska J. F. Respiratory syncytial virus proteins: identification by immunoprecipitation. J Virol. 1981 Apr;38(1):278–285. doi: 10.1128/jvi.38.1.278-285.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Buynak E. B., Weibel R. E., Carlson A. J., McLean A. A., Hilleman M. R. Further investigations of live respiratory syncytial virus vaccine administered parenterally. Proc Soc Exp Biol Med. 1979 Feb;160(2):272–277. doi: 10.3181/00379727-160-40433. [DOI] [PubMed] [Google Scholar]
  5. CHANOCK R., ROIZMAN B., MYERS R. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am J Hyg. 1957 Nov;66(3):281–290. doi: 10.1093/oxfordjournals.aje.a119901. [DOI] [PubMed] [Google Scholar]
  6. Campbell J. J., Cosby S. L., Scott J. K., Rima B. K., Martin S. J., Appel M. A comparison of measles and canine distemper virus polypeptides. J Gen Virol. 1980 May;48(1):149–159. doi: 10.1099/0022-1317-48-1-149. [DOI] [PubMed] [Google Scholar]
  7. Coates H. V., Alling D. W., Chanock R. M. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am J Epidemiol. 1966 Mar;83(2):299–313. doi: 10.1093/oxfordjournals.aje.a120586. [DOI] [PubMed] [Google Scholar]
  8. Cote P. J., Jr, Fernie B. F., Ford E. C., Shih J. W., Gerin J. L. Monoclonal antibodies to respiratory syncytial virus: detection of virus neutralization and other antigen-antibody systems using infected human and murine cells. J Virol Methods. 1981 Oct;3(3):137–147. doi: 10.1016/0166-0934(81)90048-3. [DOI] [PubMed] [Google Scholar]
  9. Dimitriadis G. J. Effect of detergents on antibody-antigen interaction. Anal Biochem. 1979 Oct 1;98(2):445–451. doi: 10.1016/0003-2697(79)90165-9. [DOI] [PubMed] [Google Scholar]
  10. Fernie B. F., Ford E. C., Gerin J. L. The development of Balb/c cells persistently infected with respiratory syncytial virus: presence of ribonucleoprotein on the cell surface. Proc Soc Exp Biol Med. 1981 May;167(1):83–86. doi: 10.3181/00379727-167-41129. [DOI] [PubMed] [Google Scholar]
  11. Fernie B. F., Gerin J. L. The stabilization and purification of respiratory syncytial virus using MgSO4. Virology. 1980 Oct 15;106(1):141–144. doi: 10.1016/0042-6822(80)90229-9. [DOI] [PubMed] [Google Scholar]
  12. Graves M. C., Silver S. M., Choppin P. W. Measles virus polypeptides synthesis in infected cells. Virology. 1978 May 1;86(1):254–263. doi: 10.1016/0042-6822(78)90025-9. [DOI] [PubMed] [Google Scholar]
  13. Hall W. W., Lamb R. A., Choppin P. W. The polypeptides of canine distemper virus: synthesis in infected cells and relatedness to the polypeptides of other morbilliviruses. Virology. 1980 Jan 30;100(2):433–449. doi: 10.1016/0042-6822(80)90534-6. [DOI] [PubMed] [Google Scholar]
  14. Jacobs J. W., Peacock D. B., Corner B. D., Caul E. O., Clarke S. K. Respiratory syncytial and other viruses associated with respiratory disease in infants. Lancet. 1971 May 1;1(7705):871–876. doi: 10.1016/S0140-6736(71)92440-8. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kennel S. J., Del Villano B. C., Levy R. L., Lerner R. A. Properties of an oncornavirus glycoprotein: evidence for its presence on the surface of virions and infected cells. Virology. 1973 Oct;55(2):464–475. doi: 10.1016/0042-6822(73)90188-8. [DOI] [PubMed] [Google Scholar]
  16. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  17. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  18. Laskey R. A., Mills A. D. Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Lett. 1977 Oct 15;82(2):314–316. doi: 10.1016/0014-5793(77)80609-1. [DOI] [PubMed] [Google Scholar]
  19. Lazarides E., Lindberg U. Actin is the naturally occurring inhibitor of deoxyribonuclease I. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4742–4746. doi: 10.1073/pnas.71.12.4742. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Melnick J. L. Classification and nomenclature of animal viruses, 1971. Prog Med Virol. 1971;13:462–484. [PubMed] [Google Scholar]
  21. Merz D. C., Scheid A., Choppin P. W. Importance of antibodies to the fusion glycoprotein of paramyxoviruses in the prevention of spread of infection. J Exp Med. 1980 Feb 1;151(2):275–288. doi: 10.1084/jem.151.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Orvell C. Immunological properties of purified mumps virus glycoproteins. J Gen Virol. 1978 Dec;41(3):517–526. doi: 10.1099/0022-1317-41-3-517. [DOI] [PubMed] [Google Scholar]
  23. Orvell C. Structural polypeptides of mumps virus. J Gen Virol. 1978 Dec;41(3):527–539. doi: 10.1099/0022-1317-41-3-527. [DOI] [PubMed] [Google Scholar]
  24. Peeples M., Levine S. Respiratory syncytial virus polypeptides: their location in the virion. Virology. 1979 May;95(1):137–145. doi: 10.1016/0042-6822(79)90408-2. [DOI] [PubMed] [Google Scholar]
  25. Pitha P. M., Fernie B., Maldarelli F., Hattman T., Wivel N. A. Effect of interferon on mouse leukaemia virus (MuLV). V. Abnormal proteins in virions of Rauscher MuLV produced in the presence of interferon. J Gen Virol. 1980 Jan;46(1):97–110. doi: 10.1099/0022-1317-46-1-97. [DOI] [PubMed] [Google Scholar]
  26. Pringle C. R., Cross A. Neutralisation of respiratory syncytial virus by cat serum. Nature. 1978 Nov 30;276(5687):501–502. doi: 10.1038/276501a0. [DOI] [PubMed] [Google Scholar]
  27. Pringle C. R., Shirodaria P. V., Gimenez H. B., Levine S. Antigen and polypeptide synthesis by temperature-sensitive mutants of respiratory syncytial virus. J Gen Virol. 1981 May;54(Pt 1):173–183. doi: 10.1099/0022-1317-54-1-173. [DOI] [PubMed] [Google Scholar]
  28. Scheid A., Choppin P. W. Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology. 1977 Jul 1;80(1):54–66. doi: 10.1016/0042-6822(77)90380-4. [DOI] [PubMed] [Google Scholar]
  29. Segrest J. P., Jackson R. L., Andrews E. P., Marchesi V. T. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis. Biochem Biophys Res Commun. 1971 Jul 16;44(2):390–395. doi: 10.1016/0006-291x(71)90612-7. [DOI] [PubMed] [Google Scholar]
  30. Tyrrell D. L., Norrby E. Structural polypeptides of measles virus. J Gen Virol. 1978 May;39(2):219–229. doi: 10.1099/0022-1317-39-2-219. [DOI] [PubMed] [Google Scholar]
  31. Wunner W. H., Pringle C. R. Respiratory syncytial virus proteins. Virology. 1976 Aug;73(1):228–243. doi: 10.1016/0042-6822(76)90077-5. [DOI] [PubMed] [Google Scholar]

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