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. 1982 Aug;37(2):463–468. doi: 10.1128/iai.37.2.463-468.1982

Immunoglobulin M responses to the Norwalk virus of gastroenteritis.

G Cukor, N A Nowak, N R Blacklow
PMCID: PMC347556  PMID: 6288563

Abstract

Eighty-seven serum specimens from 20 human subjects experimentally inoculated one or more times with Norwalk virus were quantitatively examined for virus-specific immunoglobulin M (IgM). A sensitive and specific radioimmunoassay for anti-Norwalk virus blocking activity was applied to whole serum and to separate IgM and IgG fractions obtained by sucrose density gradient ultracentrifugation. The peak IgM response occurred at about 2 weeks after illness, but IgM was detectable at lower titers for up to 21 weeks after infection. The IgM response was seen in volunteers who became ill, whether or not prechallenge total serum antibody was present. On long-term (27 to 42 months) rechallenge, volunteers who were previously ill and had produced IgM antibody again developed illness, and a secondary IgM response greater than the first was detected. Inoculated volunteers who did not develop illness, as well as previously ill volunteers on short-term rechallenge (4 to 14 weeks), usually failed to generate an IgM response, whether or not an IgG response had occurred. In ill subjects, the rise in IgM and IgG occurred concomitantly. Virus-specific IgM is not necessarily indicative of primary infection with Norwalk agent inasmuch as reinfection produces an enhancement of the IgM response. Furthermore, Norwalk-specific IgM responses do not appear to be associated with subclinical illness.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blacklow N. R., Cukor G., Bedigian M. K., Echeverria P., Greenberg H. B., Schreiber D. S., Trier J. S. Immune response and prevalence of antibody to Norwalk enteritis virus as determined by radioimmunoassay. J Clin Microbiol. 1979 Dec;10(6):903–909. doi: 10.1128/jcm.10.6.903-909.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blacklow N. R., Cukor G. Viral gastroenteritis. N Engl J Med. 1981 Feb 12;304(7):397–406. doi: 10.1056/NEJM198102123040705. [DOI] [PubMed] [Google Scholar]
  3. Dörries R., ter Meulen V. Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay. Med Microbiol Immunol. 1980;168(3):159–171. doi: 10.1007/BF02122850. [DOI] [PubMed] [Google Scholar]
  4. Greenberg H. B., Wyatt R. G., Valdesuso J., Kalica A. R., London W. T., Chanock R. M., Kapikian A. Z. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. doi: 10.1002/jmv.1890020204. [DOI] [PubMed] [Google Scholar]
  5. Minor T. E., Helstrom P. B., Nelson D. B., D'Alessio D. J. Counterimmunoelectrophoresis test for immunoglobulin M antibodies to group B coxsackievirus. J Clin Microbiol. 1979 Apr;9(4):503–506. doi: 10.1128/jcm.9.4.503-506.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mortimer P. P., Parry J. V., Appleton H. Diagnosis of recent hepatitis A infection: a comparison of two methods for detecting specific IgM. J Hyg (Lond) 1981 Feb;86(1):71–78. doi: 10.1017/s0022172400068753. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Osterholm M. T., Kantor R. J., Bradley D. W., Hall W. N., Francis D. P., Aaron H. C., Washburn J. W., Velde D. Immunoglobulin M-specific serologic testing in an outbreak of foodborne viral hepatitis, type A. Am J Epidemiol. 1980 Jul;112(1):8–16. doi: 10.1093/oxfordjournals.aje.a112978. [DOI] [PubMed] [Google Scholar]
  8. Parrino T. A., Schreiber D. S., Trier J. S., Kapikian A. Z., Blacklow N. R. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 1977 Jul 14;297(2):86–89. doi: 10.1056/NEJM197707142970204. [DOI] [PubMed] [Google Scholar]
  9. Schmidt N. J., Lennette E. H., Dennis J. Characterization of antibodies produced in natural and experimental coxsackievirus infections. J Immunol. 1968 Jan;100(1):99–106. [PubMed] [Google Scholar]
  10. Schreiber D. S., Blacklow N. R., Trier J. S. The mucosal lesion of the proximal small intestine in acute infectious nonbacterial gastroenteritis. N Engl J Med. 1973 Jun 21;288(25):1318–1323. doi: 10.1056/NEJM197306212882503. [DOI] [PubMed] [Google Scholar]
  11. Schrohenloher R. E. Copper-catalysed reoxidation of human monoclonal IgM. Scand J Immunol. 1978;8(5):443–449. doi: 10.1111/j.1365-3083.1978.tb00540.x. [DOI] [PubMed] [Google Scholar]
  12. Sheridan J. F., Aurelian L., Barbour G., Santosham M., Sack R. B., Ryder R. W. Traveler's diarrhea associated with rotavirus infection: analysis of virus-specific immunoglobulin classes. Infect Immun. 1981 Jan;31(1):419–429. doi: 10.1128/iai.31.1.419-429.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Snydman D. R., Dienstag J. L., Stedt B., Brink E. W., Ryan D. M., Fawaz K. A. Use of IgM-hepatitis A antibody testing. Investigating a common-source, food borne outbreak. JAMA. 1981 Feb 27;245(8):827–830. [PubMed] [Google Scholar]
  14. Widerlite L., Trier J. S., Blacklow N. R., Schreiber D. S. Structure of the gastric mucosa in acute infectious bacterial gastroenteritis. Gastroenterology. 1975 Mar;68(3):425–430. [PubMed] [Google Scholar]
  15. Yolken R. H., Wyatt R. G., Kim H. W., Kapikian A. Z., Chanock R. M. Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. Infect Immun. 1978 Feb;19(2):540–546. doi: 10.1128/iai.19.2.540-546.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]

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