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. 1982 Aug;37(2):481–485. doi: 10.1128/iai.37.2.481-485.1982

Rates in vitro changes of gonococcal colony opacity phenotypes.

L W Mayer
PMCID: PMC347559  PMID: 6126433

Abstract

The rate of change of colony opacity phenotype was determined for 12 strains of Neisseria gonorrhoeae. The average rate of change was about 2 X 10(-3) per colony-forming unit per generation with a range of 0.2 X 10(-3) to 4 X 10(-3) per colony-forming unit per generation. Transition from opaque to transparent occurred at the same rate as transition from transparent to opaque. The following factors were shown to have no effect on the transition rate: (i) the state of piliation; (ii) the number of passages as a particular phenotype; (iii) alteration in the temperature, pH, or amount of oxygen in the atmosphere during growth; (iv) the addition of any of 194 compounds or mixtures to the growth media; (v) the addition of DNase or of DNA from opaque or transparent gonococci; and (vi) incubation between the opacity-transparency transition and the change resulting in the loss of piliation was seen. Some implications of the high transition rate are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake M. S., Gotschlich E. C., Swanson J. Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun. 1981 Jul;33(1):212–222. doi: 10.1128/iai.33.1.212-222.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dillon J. R., Pauzé M. Relationship between plasmid content and auxotype in Neisseria gonorrhoeae isolates. Infect Immun. 1981 Aug;33(2):625–628. doi: 10.1128/iai.33.2.625-628.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Draper D. L., James J. F., Brooks G. F., Sweet R. L. Comparison of virulence markers of peritoneal and fallopian tube isolates with endocervical Neisseria gonorrhoeae isolates from women with acute salpingitis. Infect Immun. 1980 Mar;27(3):882–888. doi: 10.1128/iai.27.3.882-888.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. James J. F., Swanson J. Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun. 1978 Jan;19(1):332–340. doi: 10.1128/iai.19.1.332-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  7. Lambden P. R., Heckels J. E., James L. T., Watt P. J. Variations in surface protein composition associated with virulence properties in opacity types of Neisseria gonorrhoeae. J Gen Microbiol. 1979 Oct;114(2):305–312. doi: 10.1099/00221287-114-2-305. [DOI] [PubMed] [Google Scholar]
  8. Lambden P. R., Robertson J. N., Watt P. J. Biological properties of two distinct pilus types produced by isogenic variants of Neisseria gonorrhoeae P9. J Bacteriol. 1980 Jan;141(1):393–396. doi: 10.1128/jb.141.1.393-396.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lowe T. L., Kraus S. J. Quantitation of Neisseria gonorrhoeae from women with gonorrhea. J Infect Dis. 1976 Jun;133(6):621–626. doi: 10.1093/infdis/133.6.621. [DOI] [PubMed] [Google Scholar]
  10. Mayer L. W., Holmes K. K., Falkow S. Characterization of plasmid deoxyribonucleic acid from Neisseria gonorrhoeae. Infect Immun. 1974 Oct;10(4):712–717. doi: 10.1128/iai.10.4.712-717.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mayer L. W., Schoolnik G. K., Falkow S. Genetic studies on Neisseria gonorrhoeae from disseminated gonococcal infections. Infect Immun. 1977 Oct;18(1):165–172. doi: 10.1128/iai.18.1.165-172.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McBride H. M., Lambden P. R., Heckels J. E., Watt P. J. The role of outer membrane proteins in the survival of Neisseria gonorrhoeae P9 within guinea-pig subcutaneous chambers. J Gen Microbiol. 1981 Sep;126(1):63–67. doi: 10.1099/00221287-126-1-63. [DOI] [PubMed] [Google Scholar]
  13. Norlander L., Davies J., Norqvist A., Normark S. Genetic basis for colonial variation in Neisseria gonorrhoeae. J Bacteriol. 1979 Jun;138(3):762–769. doi: 10.1128/jb.138.3.762-769.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Roberts M., Piot P., Falkow S. The ecology of gonococcal plasmids. J Gen Microbiol. 1979 Oct;114(2):491–494. doi: 10.1099/00221287-114-2-491. [DOI] [PubMed] [Google Scholar]
  15. Salit I. E., Blake M., Gotschlich E. C. Intra-strain heterogeneity of gonococcal pili is related to opacity colony variance. J Exp Med. 1980 Mar 1;151(3):716–725. doi: 10.1084/jem.151.3.716. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salit I. E., Gotschlich E. C. Gonococcal color and opacity variants: virulence for chicken embryos. Infect Immun. 1978 Nov;22(2):359–364. doi: 10.1128/iai.22.2.359-364.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Swanson J. Colony opacity and protein II compositions of gonococci. Infect Immun. 1982 Jul;37(1):359–368. doi: 10.1128/iai.37.1.359-368.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Swanson J. Studies on gonococcus infection. XII. Colony color and opacity varienats of gonococci. Infect Immun. 1978 Jan;19(1):320–331. doi: 10.1128/iai.19.1.320-331.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Swanson J. Studies on gonococcus infection. XIV. Cell wall protein differences among color/opacity colony variants of Neisseria gonorrhoeae. Infect Immun. 1978 Jul;21(1):292–302. doi: 10.1128/iai.21.1.292-302.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Virji M., Everson J. S. Comparative virulence of opacity variants of Neisseria gonorrhoeae strain P9. Infect Immun. 1981 Mar;31(3):965–970. doi: 10.1128/iai.31.3.965-970.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Zieg J., Silverman M., Hilmen M., Simon M. Recombinational switch for gene expression. Science. 1977 Apr 8;196(4286):170–172. doi: 10.1126/science.322276. [DOI] [PubMed] [Google Scholar]

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