Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Aug;37(2):532–538. doi: 10.1128/iai.37.2.532-538.1982

Binding characteristics of wild mouse type C virus to mouse spinal cord and spleen cells.

S Mohan, B K Pal
PMCID: PMC347567  PMID: 6288566

Abstract

Binding characteristics of mouse spinal cord and spleen cells to naturally occurring, ecotropic (paralytogenic and lymphomagenic 1504M virus) and amphotropic (lymphomagenic 1504A virus) retroviruses of wild mice were investigated. 125I-labeled ecotropic (N-tropic) virus bound efficiently to both spinal cord and spleen cells from SWR/J mice (Fv-1n), but labeled amphotropic (N-tropic) virus bound efficiently only to spleen cells. The extent of binding of 1504M virus to the spinal cord cells was related to the time of incubation and to the amount of labeled input virus. 1504M virus bound to both glial and neuronal subpopulations of the spinal cord with almost equal efficiency. Binding of 125I-labeled 1504M virus to SWR/J mouse spinal cord cells was competitively inhibited by unlabeled homologous virus, whereas an excess of unlabeled 1504A virus inhibited only 10% of the ecotropic virus binding. Unlabeled 1504M virus almost completely inhibited the low-level binding of 125I-labeled 1504A virus to spinal cord cells. The different extent of binding of 1504M virus to spinal cord cells from different strains of mice (SWR/J, NIH Swiss, BALB/c-Jax, Lake Casitas wild, and CD-1) correlated with the susceptibility to paralysis in these strains of mice after inoculation with 1504M virus. Although the spinal cord cells of BALB/c mice contained a moderate amount of 1504M virus receptor sites, these mice were resistant to virus-induced paralysis because of their Fv-1b genotype. Our results indicate that the receptor sites for wild mouse ecotropic retrovirus are strain and organ specific and suggest that the presence of such receptors in central nervous system tissue may be a prerequisite for a successful virus infection and paralysis induction in Fv-1n mice.

Full text

PDF
532

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews J. M., Gardner M. B. Lower motor neuron degeneration associated with type C RNA virus infection in mice: neuropathological features. J Neuropathol Exp Neurol. 1974 Apr;33(2):285–307. doi: 10.1097/00005072-197404000-00007. [DOI] [PubMed] [Google Scholar]
  2. Brooks B. R., Swarz J. R., Johnson R. T. Spongiform polioencephalomyelopathy caused by a murine retrovirus. I. Pathogenesis of infection in newborn mice. Lab Invest. 1980 Nov;43(5):480–486. [PubMed] [Google Scholar]
  3. Bryant M. L., Klement V. Clonal heterogeneity of wild mouse leukemia viruses: host ranges and antigenicity. Virology. 1976 Sep;73(2):532–536. doi: 10.1016/0042-6822(76)90415-3. [DOI] [PubMed] [Google Scholar]
  4. Bryant M. L., Pal B. K., Gardner M. B., Elder J. H., Jensen F. C., Lerner R. A. Structural analysis of the major envelope glycoprotein (gp70) of the amphotropic and ecotropic type C viruses of wild mice. Virology. 1978 Feb;84(2):348–358. doi: 10.1016/0042-6822(78)90254-4. [DOI] [PubMed] [Google Scholar]
  5. Bryant M. L., Scott J. L., Pal B. K., Estes J. D., Gardner M. B. Immunopathology of natural and experimental lymphomas induced by wild mouse leukemia virus. Am J Pathol. 1981 Sep;104(3):272–282. [PMC free article] [PubMed] [Google Scholar]
  6. Capps-Covey P., McIlwain D. L. Bulk isolation of large ventral spinal neurons. J Neurochem. 1975 Oct;25(4):517–521. doi: 10.1111/j.1471-4159.1975.tb04358.x. [DOI] [PubMed] [Google Scholar]
  7. Dales S. Early events in cell-animal virus interactions. Bacteriol Rev. 1973 Jun;37(2):103–135. doi: 10.1128/br.37.2.103-135.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Devare S. G., Stephenson J. R. Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. J Virol. 1977 Aug;23(2):443–447. doi: 10.1128/jvi.23.2.443-447.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Duran-Troise G., Bassin R. H., Wallace B. F., Rein A. Balb/3T3 cells chronically infected with N-tropic murine leukemia virus continue to express Fv-1b restriction. Virology. 1981 Jul 30;112(2):795–799. doi: 10.1016/0042-6822(81)90329-9. [DOI] [PubMed] [Google Scholar]
  10. Fowler A. K., Twardzik D. R., Reed C. D., Weislow O. S., Hellman A. Binding characteristics of Rauscher leukemia virus envelope glycoprotein gp71 to murine lymphoid cells. J Virol. 1977 Dec;24(3):729–735. doi: 10.1128/jvi.24.3.729-735.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gardner M. B., Henderson B. E., Officer J. E., Rongey R. W., Parker J. C., Oliver C., Estes J. D., Huebner R. J. A spontaneous lower motor neuron disease apparently caused by indigenous type-C RNA virus in wild mice. J Natl Cancer Inst. 1973 Oct;51(4):1243–1254. doi: 10.1093/jnci/51.4.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gardner M. B., Klement V., Rongey R. R., McConahey P., Estes J. D., Huebner R. J. Type C virus expression in lymphoma-paralysis-prone wild mice. J Natl Cancer Inst. 1976 Sep;57(3):585–590. doi: 10.1093/jnci/57.3.585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gardner M. B. Type C viruses of wild mice: characterization and natural history of amphotropic, ecotropic, and xenotropic MuLv. Curr Top Microbiol Immunol. 1978;79:215–259. doi: 10.1007/978-3-642-66853-1_5. [DOI] [PubMed] [Google Scholar]
  14. Hartley J. W., Rowe W. P. Naturally occurring murine leukemia viruses in wild mice: characterization of a new "amphotropic" class. J Virol. 1976 Jul;19(1):19–25. doi: 10.1128/jvi.19.1.19-25.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hazama H., Uchimura H. Bulk separation of neurons and glia from small amounts of brain tissue. Exp Cell Res. 1973 Dec;82(2):452–454. doi: 10.1016/0014-4827(73)90364-9. [DOI] [PubMed] [Google Scholar]
  16. Jolicoeur P., Baltimore D. Effect of Fv-1 gene product on proviral DNA formation and integration in cells infected with murine leukemia viruses. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2236–2240. doi: 10.1073/pnas.73.7.2236. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Jolicoeur P. The Fv-1 gene of the mouse and its control of murine leukemia virus replication. Curr Top Microbiol Immunol. 1979;86:67–122. doi: 10.1007/978-3-642-67341-2_3. [DOI] [PubMed] [Google Scholar]
  18. Kalyanaraman V. S., Sarngadharan M. G., Gallo R. C. Characterization of Rauscher murine leukemia virus envelope glycoprotein receptor in membranes from murine fibroblasts. J Virol. 1978 Dec;28(3):686–696. doi: 10.1128/jvi.28.3.686-696.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Miyamoto K., Gilden R. V. Electron microscopic studies of tumor viruses. I. Entry of murine leukemia virus into mouse embryo fibroblasts. J Virol. 1971 Mar;7(3):395–406. doi: 10.1128/jvi.7.3.395-406.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oldstone M. B., Jensen F., Dixon F. J., Lampert P. W. Pathogenesis of the slow disease of the central nervous system associated with wild mouse virus. II. Role of virus and host gene products. Virology. 1980 Nov;107(1):180–193. doi: 10.1016/0042-6822(80)90283-4. [DOI] [PubMed] [Google Scholar]
  21. Pal B. K., Wright M., Officer J. E., Gardner M. B., Roy-Burman P. Subviral components of a wild mouse embryo-derived type C oncornavirus. Virology. 1973 Nov;56(1):189–197. doi: 10.1016/0042-6822(73)90298-5. [DOI] [PubMed] [Google Scholar]
  22. Rasheed S., Gardner M. B., Chan E. Amphotropic host range of naturally occuring wild mouse leukemia viruses. J Virol. 1976 Jul;19(1):13–18. doi: 10.1128/jvi.19.1.13-18.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rasheed S., Toth E., Gardner M. B. Characterization of purely ecotropic and amphotropic naturally occurring wild mouse leukemia viruses. Intervirology. 1977;8(6):323–335. doi: 10.1159/000148908. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES