Abstract
Antisera against isolated cell surface preparations (PCSP-As) of Leishmania donovani promastigotes were used to detect extracellular antigens produced during the growth of these organisms in four different growth media. The PCSP-As precipitated two major antigenically identical but electrophoretically distinct components, in addition to several minor antigens. Immunoelectrophoretic studies employing PCSP-As, PCSP-As absorbed with intact, live promastigotes, and PCSP-As absorbed with a major extracellular antigen demonstrated the antigenic identity between the major extracellular antigens and two major components externally disposed at the surface of promastigotes. Growth curve kinetic investigations suggested that the major extracellular antigens did not appear in the growth media primarily as a result of cell lysis or damage. The carbohydrate nature of the major extracellular antigens was indicated by physicochemical characterization.
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Selected References
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- Annual meeting of the Israel Society for Parasitology, 23 April 1980, Jerusalem. Abstracts of papers. Isr J Med Sci. 1980 Jul;16(7):559–562. [PubMed] [Google Scholar]
- Bailey C. F., Bowers B. Localization of lipophosphonoglycan in membranes of Acanthamoeba by using specific antibodies. Mol Cell Biol. 1981 Apr;1(4):358–369. doi: 10.1128/mcb.1.4.358. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berman J. D., Dwyer D. M. Expression of Leishmania antigen on the surface membrane of infected human macrophages in vitro. Clin Exp Immunol. 1981 May;44(2):342–348. [PMC free article] [PubMed] [Google Scholar]
- Berman J. D., Dwyer D. M., Wyler D. J. Multiplication of Leishmania in human macrophages in vitro. Infect Immun. 1979 Oct;26(1):375–379. doi: 10.1128/iai.26.1.375-379.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bowers B., Korn E. D. Localization of lipophosphonoglycan on both sides of Acanthamoeba plasma membrane. J Cell Biol. 1974 Aug;62(2):533–540. doi: 10.1083/jcb.62.2.533. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang K. P., Dwyer D. M. Leishmania donovani. Hamster macrophage interactions in vitro: cell entry, intracellular survival, and multiplication of amastigotes. J Exp Med. 1978 Feb 1;147(2):515–530. doi: 10.1084/jem.147.2.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Clinton B. A., Palczuk N. C., Stauber L. A. Leishmania donovani; partial characterization of some flagellate cytoplasmic immunogens. J Immunol. 1972 Jun;108(6):1570–1577. [PubMed] [Google Scholar]
- Decker-Jackson J. E., Honigberg B. M. Glycoproteins released by Leishmania donovani: immunologic relationships with host and bacterial antigens and preliminary biochemical analysis. J Protozool. 1978 Nov;25(4):514–525. doi: 10.1111/j.1550-7408.1978.tb04178.x. [DOI] [PubMed] [Google Scholar]
- Dwyer D. M. Analysis of the antigenic relationships among Trichomonas, Histomonas, Dientamoeba, and Entamoeba. II. Gel diffusion methods. J Protozool. 1972 May;19(2):326–332. doi: 10.1111/j.1550-7408.1972.tb03468.x. [DOI] [PubMed] [Google Scholar]
- Dwyer D. M. Antibody-induced modulation of Leishmania donovani surface membrane antigens. J Immunol. 1976 Dec;117(6):2081–2091. [PubMed] [Google Scholar]
- Dwyer D. M. Isolation and partial characterization of surface membranes from Leishmania donovani promastigotes. J Protozool. 1980 May;27(2):176–182. doi: 10.1111/j.1550-7408.1980.tb04676.x. [DOI] [PubMed] [Google Scholar]
- Dwyer D. M. Leishmania donovani: surface membrane carbohydrates of promastigotes. Exp Parasitol. 1977 Apr;41(2):341–358. doi: 10.1016/0014-4894(77)90107-2. [DOI] [PubMed] [Google Scholar]
- El-On J., Bradley D. J., Freeman J. C. Leishmania donovani: action of excreted factor on hydrolytic enzyme activity of macrophages from mice with genetically different resistance to infection. Exp Parasitol. 1980 Apr;49(2):167–174. doi: 10.1016/0014-4894(80)90114-9. [DOI] [PubMed] [Google Scholar]
- El-On J., Schnur L. F., Greenblatt C. L. Leishmania donovani: physicochemical, immunological, and biological characterization of excreted factor from promastigotes. Exp Parasitol. 1979 Apr;47(2):254–269. doi: 10.1016/0014-4894(79)90078-x. [DOI] [PubMed] [Google Scholar]
- Gottlieb M. A carbohydrate-containing antigen from Trypanosoma cruzi and its detection in the circulation of infected mice. J Immunol. 1977 Aug;119(2):465–470. [PubMed] [Google Scholar]
- Gottlieb M., Dwyer D. M. Leishmania donovani: surface membrane acid phosphatase activity of promastigotes. Exp Parasitol. 1981 Aug;52(1):117–128. doi: 10.1016/0014-4894(81)90067-9. [DOI] [PubMed] [Google Scholar]
- Handman E., Greenblatt C. L. Promotion of leishmanial infections in non-permissive host macrophages by conditioned medium. Z Parasitenkd. 1977 Sep 21;53(2):143–147. doi: 10.1007/BF00380458. [DOI] [PubMed] [Google Scholar]
- Hendricks L. D., Wood D. E., Hajduk M. E. Haemoflagellates: commercially available liquid media for rapid cultivation. Parasitology. 1978 Jun;76(3):309–316. doi: 10.1017/s0031182000048186. [DOI] [PubMed] [Google Scholar]
- Schnur L. F., Zuckerman A., Greenblatt C. L. Leishmanial serotypes as distinguished by the gel diffusion of factors excreted in vitro and in vivo. Isr J Med Sci. 1972 Jul;8(7):932–942. [PubMed] [Google Scholar]
- Semprevivo L. H. Exometabolites of Leishmania donovani promastigotes. I. Isolation and initial characterization. Proc Soc Exp Biol Med. 1978 Oct;159(1):105–110. doi: 10.3181/00379727-159-40293. [DOI] [PubMed] [Google Scholar]
- Semprevivo L. H., Honigberg B. M. Exometabolites of Leishmania donovani promastigotes. II. Spontaneous changes of exometabolite after isolation. Z Parasitenkd. 1980;62(3):201–211. doi: 10.1007/BF00926562. [DOI] [PubMed] [Google Scholar]
- Shaw J. J., Lainson R. Leishmaniasis in Brazil: X. Some observations of intradermal reactions to different trypanosomatid antigens of patients suffering from cutaneous and mucocutaneous leishmaniasis. Trans R Soc Trop Med Hyg. 1975;69(3):323–335. doi: 10.1016/0035-9203(75)90127-3. [DOI] [PubMed] [Google Scholar]
- Slutzky G. M., El-On J., Greenblatt C. L. Leishmanial excreted factor: protein-bound and free forms from promastigote cultures of Leishmania tropica and Leishmania donovani. Infect Immun. 1979 Dec;26(3):916–924. doi: 10.1128/iai.26.3.916-924.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slutzky G. M., Greenblatt C. L. Analysis by SDS-polyacrylamide gel electrophoresis of an immunologically active factor of Leishmania tropica from growth media, promastigotes, and infected macrophages. Biochem Med. 1979 Feb;21(1):70–77. doi: 10.1016/0006-2944(79)90057-7. [DOI] [PubMed] [Google Scholar]
- Steiger R. F., Black C. D. Simplified defined media for cultivating Leishmania donovani promastigotes. Acta Trop. 1980 Jun;37(2):195–198. [PubMed] [Google Scholar]
- Steiger R. F., Steiger E. Cultivation of Leishmania donovani and Leishmania braziliensis in defined media: nutritional requirements. J Protozool. 1977 Aug;24(3):437–441. doi: 10.1111/j.1550-7408.1977.tb04771.x. [DOI] [PubMed] [Google Scholar]
- Svendsen J., Axelsen N. H. A modified antigen--antibody crossed electrophoresis characterizing the specificity and titre of human precipiting against Candida albicans. J Immunol Methods. 1972 Jan;1(2):169–176. doi: 10.1016/0022-1759(72)90044-0. [DOI] [PubMed] [Google Scholar]
- Weeke B. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures. Scand J Immunol Suppl. 1973;1:15–35. doi: 10.1111/j.1365-3083.1973.tb03776.x. [DOI] [PubMed] [Google Scholar]