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. 1982 Aug;37(2):656–661. doi: 10.1128/iai.37.2.656-661.1982

Effects of local immunization with glucosyltransferase on colonization of hamsters by Streptococcus mutans.

D J Smith, M A Taubman, J L Ebersole
PMCID: PMC347582  PMID: 6214510

Abstract

Experiments were performed to study the effect of antibody to Streptococcus mutans glucosyltransferase (GTF) on the implantation of these organisms in hamsters. Salivary (immunoglobulin A) and serum (immunoglobulin G) antibodies to GTF and GTF-inhibiting activity were elicited by injection of GTF in Freund complete adjuvant in the salivary gland region. Sham-immunized and GTF-immunized groups were then orally challenged with approximately 10(7), 10(8), or 10(9) colony-forming units of cariogenic S. mutans 6715. The results were evaluated by systematically swabbing molars 4 days and approximately 4 weeks after challenge. In general, fewer GTF-immunized hamsters became infected with S. mutans after challenge with 10(7) or 10(8) organisms than did identically challenged sham-immunized hamsters. Of the animals that did become infected, fewer S. mutans colony-forming units were recovered from GTF-immunized hamsters. These results indicate that the presence of antibody to GTF can diminish the ability of S. mutans to implant in the oral cavity of immunized hamsters.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark W. B., Gibbons R. J. Influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of Streptococcus mutans 6715 to hydroxyapatite surfaces. Infect Immun. 1977 Nov;18(2):514–523. doi: 10.1128/iai.18.2.514-523.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ebersole J. L., Taubman M. A., Smith D. J. Thymic control of secretory antibody responses in the rat. J Immunol. 1979 Jul;123(1):19–24. [PubMed] [Google Scholar]
  3. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  4. Janda W. M., Kuramitsu H. K. Properties of a variant of Streptococcus mutans altered in its ability to interact with glucans. Infect Immun. 1977 May;16(2):575–586. doi: 10.1128/iai.16.2.575-586.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KEYES P. H., JORDAN H. V. PERIODONTAL LESIONS IN THE SYRIAN HAMSTER. III. FINDINGS RELATED TO AN INFECTIOUS AND TRANSMISSIBLE COMPONENT. Arch Oral Biol. 1964 Jul-Aug;9:377–400. doi: 10.1016/0003-9969(64)90024-x. [DOI] [PubMed] [Google Scholar]
  6. Kuramitsu H., Ingersoll L. Molecular basis for the different sucrose-dependent adherence properties of Streptococcus mutans and Streptococcus sanguis. Infect Immun. 1977 Aug;17(2):330–337. doi: 10.1128/iai.17.2.330-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Liljemark W. F., Bloomquist C. G., Germaine G. R. Effect of bacterial aggregation on the adherence of oral streptococci to hydroxyapatite. Infect Immun. 1981 Mar;31(3):935–941. doi: 10.1128/iai.31.3.935-941.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rölla G., Iversen O. J., Bonesvoll P. Lipoteichoic acid - the key to the adhesiveness of sucrose grown Streptococcus mutans. Adv Exp Med Biol. 1978;107:607–617. doi: 10.1007/978-1-4684-3369-2_69. [DOI] [PubMed] [Google Scholar]
  10. Schachtele C. F., Harlander S. K., Germaine G. R. Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. Infect Immun. 1976 May;13(5):1522–1524. doi: 10.1128/iai.13.5.1522-1524.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schachtele C. F., Staat R. H., Harlander S. K. Dextranases from oral bacteria: inhibition of water-insoluble glucan production and adherence to smooth surfaces by Streptococcus mutans. Infect Immun. 1975 Aug;12(2):309–317. doi: 10.1128/iai.12.2.309-317.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith D. J., Taubman M. A. Antigenic relatedness of glucosyltransferase enzymes from streptococcus mutans. Infect Immun. 1977 Jan;15(1):91–103. doi: 10.1128/iai.15.1.91-103.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Smith D. J., Taubman M. A., Ebersole J. L. Effect of oral administration of glucosyltransferase antigens on experimental dental caries. Infect Immun. 1979 Oct;26(1):82–89. doi: 10.1128/iai.26.1.82-89.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith D. J., Taubman M. A., Ebersole J. L. Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in hamsters caused by homologous and heterologous serotypes of Streptococcus mutans. Infect Immun. 1978 Sep;21(3):843–851. doi: 10.1128/iai.21.3.843-851.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Smith D. J., Taubman M. A., Ebersole J. L. Local and systemic antibody response to oral administration of glucosyltransferase antigen complex. Infect Immun. 1980 May;28(2):441–450. doi: 10.1128/iai.28.2.441-450.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith D. J., Taubman M. A., Ebersole J. L. Preparation of glucosyltransferase from Streptococcus mutans by elution from water-insoluble polysaccharide with a dissociating solvent. Infect Immun. 1979 Feb;23(2):446–452. doi: 10.1128/iai.23.2.446-452.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Staat R. H., Langley S. D., Doyle R. J. Streptococcus mutans adherence: presumptive evidence for protein-mediated attachment followed by glucan-dependent cellular accumulation. Infect Immun. 1980 Feb;27(2):675–681. doi: 10.1128/iai.27.2.675-681.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Taubman M. A., Smith D. J. Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in rats and hamsters. J Immunol. 1977 Feb;118(2):710–720. [PubMed] [Google Scholar]
  19. Tinanoff N., Tanzer J. M., Freedman M. L. In vitro colonization of Streptococcus mutans on enamel. Infect Immun. 1978 Sep;21(3):1010–1019. doi: 10.1128/iai.21.3.1010-1019.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Van Houte J., Upeslacis V. N. Studies of the mechanism of sucrose-associated colonization of Streptococcus mutans on teeth of conventional rats. J Dent Res. 1976 Mar-Apr;55(2):216–222. doi: 10.1177/00220345760550020901. [DOI] [PubMed] [Google Scholar]
  21. Wu-Yuan C. D., Tai S., Slade H. D. Properties of Streptococcus mutans grown in a synthetic medium: binding of glucosyltransferase and in vitro adherence, and binding of dextran/glucan and glycoprotein and agglutination. Infect Immun. 1979 Mar;23(3):600–608. doi: 10.1128/iai.23.3.600-608.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Houte J., Burgess R. C., Onose H. Oral implantation of human strains of Streptococcus mutans in rats fed sucrose or glucose diets. Arch Oral Biol. 1976;21(9):561–564. doi: 10.1016/0003-9969(76)90023-6. [DOI] [PubMed] [Google Scholar]

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