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. 1982 Sep;37(3):956–960. doi: 10.1128/iai.37.3.956-960.1982

K antigen and serum sensitivity of rough Escherichia coli.

S Opal, A Cross, P Gemski
PMCID: PMC347631  PMID: 6752031

Abstract

We prepared bacterial hybrids which express both K1 and K27 antigens and examined the relative contributions of these capsules to serum resistance. Escherichia coli Hfr strain F639 (rough, K27+, serum sensitive) was conjugated with E. coli recipient strain E412 (rough, K1+, His- Trp- Strr, serum resistant). Transconjugants which inherited both the his and trp linked genes for K27 antigen synthesis were analyzed. These hybrids retained and expressed the K1 antigen since the K1 locus is nonallelic with K27 gene loci. Hybrid strains which express both K1 and K27 antigens exhibit serum resistance, but not at the level of the K1+ parental strain. An isogenic K1 derivative of a hybrid which expressed only K27 antigen was serum sensitive (greater than 99% kill, 60 min). These findings indicate that the presence of the K1 capsular antigen can protect some rough strains of E. coli from serum bactericidal activity, whereas K27 and perhaps other K antigens fail to provide such a protective effect.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns M. M., Davies D. L., Hardy K. G. Cloned fragments of the plasmid ColV,I-K94 specifying virulence and serum resistance. Nature. 1979 Jun 28;279(5716):778–781. doi: 10.1038/279778a0. [DOI] [PubMed] [Google Scholar]
  2. Feingold D. S. The serum bactericidal reaction. IV. Phenotypic conversion of Escherichia coli from serum-resistance to serum-sensitivity by diphenylamine. J Infect Dis. 1969 Oct;120(4):437–444. doi: 10.1093/infdis/120.4.437. [DOI] [PubMed] [Google Scholar]
  3. Glynn A. A., Howard C. J. The sensitivity to complement of strains of Escherichia coli related to their K antigens. Immunology. 1970 Mar;18(3):331–346. [PMC free article] [PubMed] [Google Scholar]
  4. Gross R. J., Cheasty T., Rowe B. Isolation of bacteriophages specific for the K1 polysaccharide antigen of Escherichia coli. J Clin Microbiol. 1977 Dec;6(6):548–550. doi: 10.1128/jcm.6.6.548-550.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Inoue K., Yonemasu K., Takamizawa A., Amano T. [Studies on the immune bacteriolysis. XIV. Requirement of all nine components of complement for immune bacteriolysis]. Biken J. 1968 Sep;11(3):203–206. [PubMed] [Google Scholar]
  6. McCracken G. H., Jr, Sarff L. D., Glode M. P., Mize S. G., Schiffer M. S., Robbins J. B., Gotschlich E. C., Orskov I., Orskov F. Relation between Escherichia coli K1 capsular polysaccharide antigen and clinical outcome in neonatal meningitis. Lancet. 1974 Aug 3;2(7875):246–250. doi: 10.1016/s0140-6736(74)91413-5. [DOI] [PubMed] [Google Scholar]
  7. Moll A., Manning P. A., Timmis K. N. Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli. Infect Immun. 1980 May;28(2):359–367. doi: 10.1128/iai.28.2.359-367.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Orskov F., Orskov I., Jann B., Jann K. Immunoelectrophoretic patterns of extracts from all Escherichia coli O and K antigen test strains: correlation with pathogenicity. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(2):142–152. doi: 10.1111/j.1699-0463.1971.tb02141.x. [DOI] [PubMed] [Google Scholar]
  9. Orskov I., Sharma V., Orskov F. Genetic mapping of the K1 and K4 antigens (L) of Escherichia coli. Non-allelism of K(L) antigens with K antigens of O8:K27(A), O8:K8(L) and O9:K57(B). Acta Pathol Microbiol Scand B. 1976 Jun;84(3):125–131. [PubMed] [Google Scholar]
  10. Reynard A. M., Beck M. E. Plasmid-mediated resistance to the bactericidal effects of normal rabbit serum. Infect Immun. 1976 Sep;14(3):848–850. doi: 10.1128/iai.14.3.848-850.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Robbins J. B., McCracken G. H., Jr, Gotschlich E. C., Orskov F., Orskov I., Hanson L. A. Escherichia coli K1 capsular polysaccharide associated with neonatal meningitis. N Engl J Med. 1974 May 30;290(22):1216–1220. doi: 10.1056/NEJM197405302902202. [DOI] [PubMed] [Google Scholar]
  12. Schiffer M. S., Oliveira E., Glode M. P., McCracken G. H., Jr, Sarff L. M., Robbins J. B. A review: relation between invasiveness and the K1 capsular polysaccharide of Escherichia coli. Pediatr Res. 1976 Feb;10(2):82–87. doi: 10.1203/00006450-197602000-00002. [DOI] [PubMed] [Google Scholar]
  13. Schmidt G., Fromme I., Mayer H. Immunochemical studies on core lipopolysaccharides of Enterobacteriaceae of different genera. Eur J Biochem. 1970 Jun;14(2):357–366. doi: 10.1111/j.1432-1033.1970.tb00297.x. [DOI] [PubMed] [Google Scholar]
  14. Schmidt G., Jann B., Jann K. Immunochemistry of R lipopolysaccharides of Escherichia coli. Different core regions in the lipopolysaccharides of O group 8. Eur J Biochem. 1969 Oct;10(3):501–510. doi: 10.1111/j.1432-1033.1969.tb00717.x. [DOI] [PubMed] [Google Scholar]
  15. Schmidt G., Jann B., Jann K., Orskov I., Orskov F. Genetic determinants of the synthesis of the polysaccharide capsular antigen K27(A) of Escherichia coli. J Gen Microbiol. 1977 Jun;100(2):355–361. doi: 10.1099/00221287-100-2-355. [DOI] [PubMed] [Google Scholar]
  16. Silver R. P., Finn C. W., Vann W. F., Aaronson W., Schneerson R., Kretschmer P. J., Garon C. F. Molecular cloning of the K1 capsular polysaccharide genes of E. coli. Nature. 1981 Feb 19;289(5799):696–698. doi: 10.1038/289696b0. [DOI] [PubMed] [Google Scholar]
  17. Smith H. W., Huggins M. B. The association of the O18, K1 and H7 antigens and the Co1V plasmid of a strain of Escherichia coli with its virulence and immunogenicity. J Gen Microbiol. 1980 Dec;121(2):387–400. doi: 10.1099/00221287-121-2-387. [DOI] [PubMed] [Google Scholar]
  18. Taylor P. W. Genetical studies of serum resistance in Escherichia coli. J Gen Microbiol. 1975 Jul;89(1):57–66. doi: 10.1099/00221287-89-1-57. [DOI] [PubMed] [Google Scholar]
  19. Taylor P. W., Hughes C. Plasmid carriage and the serum sensitivity of enterobacteria. Infect Immun. 1978 Oct;22(1):10–17. doi: 10.1128/iai.22.1.10-17.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Taylor P. W., Parton R. A protein factor associated with serum resistance in Escherichia coli. J Med Microbiol. 1977 May;10(2):225–232. doi: 10.1099/00222615-10-2-225. [DOI] [PubMed] [Google Scholar]
  21. Taylor P. W., Robinson M. K. Determinants that increase the serum resistance of Escherichia coli. Infect Immun. 1980 Jul;29(1):278–280. doi: 10.1128/iai.29.1.278-280.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Wilkinson R. G., Gemski P., Jr, Stocker B. A. Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. J Gen Microbiol. 1972 May;70(3):527–554. doi: 10.1099/00221287-70-3-527. [DOI] [PubMed] [Google Scholar]
  23. Wolberg G., DeWitt C. W. Mouse virulence of K(L) antigen-containing strains of Escherichia coli. J Bacteriol. 1969 Nov;100(2):730–737. doi: 10.1128/jb.100.2.730-737.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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