Abstract
Four isolates of Staphylococcus aureus from patients with endocarditis and bacteremia were capable of secreting high-molecular-weight soluble peptidoglycans when grown in a minimal cell wall medium containing penicillin G. Vancomycin was not able to substitute for penicillin G in triggering this secretion. Secretion reflected de novo synthesis of soluble peptidoglycan and was strongly dependent on time of incubation (30 to 60 min), and number of bacteria (2 X 10(8) to 5 X 10(8) colony-forming units per ml), but not on penicillin G concentration (10 to 250 micrograms/ml). The incorporation of alanine into the peptidoglycans secreted in vitro by these isolates incubated in the presence of penicillin G under optimal conditions was variable. The least incorporation of alanine into peptidoglycan occurred with an isolate from a patient treated with nafcillin who had no detectable antipeptidoglycan titer.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- CHATTERJEE A. N., PARK J. T. BIOSYNTHESIS OF CELL WALL MUCOPEPTIDE BY A PARTICULATE FRACTION FROM STAPHYLOCOCCUS AUREUS. Proc Natl Acad Sci U S A. 1964 Jan;51:9–16. doi: 10.1073/pnas.51.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DICKINSON J. C., ROSENBLUM H., HAMILTON P. B. ION EXCHANGE CHROMATOGRAPHY OF THE FREE AMINO ACIDS IN THE PLASMA OF THE NEWBORN INFANT. Pediatrics. 1965 Jul;36:2–13. [PubMed] [Google Scholar]
- Keglević D., Ladesić B., Hadzija O., Tomasić J., Valinger Z., Pokorny M., Naumski R. Isolation and study of the composition of a peptidoglycan complex excreted by the biotin-requiring mutant of Brevibacterium divaricatum NRRL-2311 in the presence of penicillin. Eur J Biochem. 1974 Mar 1;42(2):389–400. doi: 10.1111/j.1432-1033.1974.tb03351.x. [DOI] [PubMed] [Google Scholar]
- Mirelman D., Bracha R., Sharon N. Penicillin-induced secretion of soluble, uncross-linked peptidoglycan by Micrococcus luteus cells. Biochemistry. 1974 Nov 19;13(24):5045–5053. doi: 10.1021/bi00721a028. [DOI] [PubMed] [Google Scholar]
- PARK J. T., STROMINGER J. L. Mode of action of penicillin. Science. 1957 Jan 18;125(3238):99–101. doi: 10.1126/science.125.3238.99. [DOI] [PubMed] [Google Scholar]
- PARK J. T. Uridine-5'-pyrophosphate derivatives. III. Amino acid-containing derivatives. J Biol Chem. 1952 Feb;194(2):897–904. [PubMed] [Google Scholar]
- Rosenthal R. S., Jungkind D., Daneo-Moore L., Shockman G. D. Evidence for the synthesis of soluble peptidoglycan fragments by protoplasts of Streptococcus faecalis. J Bacteriol. 1975 Oct;124(1):398–409. doi: 10.1128/jb.124.1.398-409.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tynecka Z., Ward J. B. Peptidoglycan synthesis in Bacillus licheniformis. The inhibition of cross-linking by benzylpenicillin and cephaloridine in vivo accompanied by the formation of soluble peptidoglycan. Biochem J. 1975 Jan;146(1):253–267. doi: 10.1042/bj1460253. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zeiger A. R., Eaton S. M., Mirelman D. Antibodies against a synthetic peptidoglycan-precursor pentapeptide cross-react with at least two distinct populations of uncross-linked soluble peptidoglycan secreted by Micrococcus luteus cells. Eur J Biochem. 1978 May;86(1):235–240. doi: 10.1111/j.1432-1033.1978.tb12304.x. [DOI] [PubMed] [Google Scholar]
- Zeiger A. R., Maurer P. H. Immunochemistry of a synthetic peptidoglycan-precursor pentapeptide. Biochemistry. 1973 Aug 28;12(18):3387–3394. doi: 10.1021/bi00742a004. [DOI] [PubMed] [Google Scholar]
- Zeiger A. R., Tuazon C. U., Sheagren J. N. Antibody levels to bacterial peptidoglycan in human sera during the time course of endocarditis and bacteremic infections caused by Staphylococcus aureus. Infect Immun. 1981 Sep;33(3):795–800. doi: 10.1128/iai.33.3.795-800.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]