Abstract
During the course of Candida albicans antigen production, a variant of this organism was encountered which did not produce hyphae at 37 degrees C. Presented here are some of the characteristics of this variant. It produces hyphae at 25 degrees C on cornmeal agar and synthetic medium plus N-acetylglucosamine and Tween 80. At 37 degrees C, it does not produce hyphae on these media, although C. albicans normally does produce hyphae under these circumstances. In liquid synthetic medium, this variant does not produce hyphae at 37 degrees C. The variant strain was analyzed for DNA, RNA, protein content, and particle size. After 50 to 70 h in balanced exponential-phase growth, particle size distribution was narrow, and there were no differences in the DNA, RNA, or protein content per particle in the two strains. When balanced exponential-phase cultures were brought into stationary phase, both strains contained the same amount of DNA per cell.
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Selected References
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- BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beheshti F., Smith A. G., Krause G. W. Germ tube and chlamydospore formation by Candida albicans on a new medium. J Clin Microbiol. 1975 Oct;2(4):345–348. doi: 10.1128/jcm.2.4.345-348.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dabrowa N., Howard D. H., Landau J. W., Shechter Y. Synthesis of nueic acids and proteins in the dimorphic forms of Candida albicans. Sabouraudia. 1970 Nov;8(3):163–169. doi: 10.1080/00362177085190831. [DOI] [PubMed] [Google Scholar]
- Evans E. G., Odds F. C., Richardson M. D., Holland K. T. Optimum conditions for initiation of filamentation in Candida albicans. Can J Microbiol. 1975 Mar;21(3):338–342. doi: 10.1139/m75-048. [DOI] [PubMed] [Google Scholar]
- Hartwell L. H. Saccharomyces cerevisiae cell cycle. Bacteriol Rev. 1974 Jun;38(2):164–198. doi: 10.1128/br.38.2.164-198.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hatzfeld J. DNA labelling and its assay in yeast. Biochim Biophys Acta. 1973 Feb 23;299(1):34–42. doi: 10.1016/0005-2787(73)90395-x. [DOI] [PubMed] [Google Scholar]
- Joshi K. R., Gavin J. B., Bremner D. A. The formation of germ tubes by Candida albicans in various peptone media. Sabouraudia. 1973 Nov;11(3):259–262. [PubMed] [Google Scholar]
- Lee K. L., Buckley H. R., Campbell C. C. An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans. Sabouraudia. 1975 Jul;13(2):148–153. doi: 10.1080/00362177585190271. [DOI] [PubMed] [Google Scholar]
- Ogletree F. F., Abdelal A. T., Ahearn D. G. Germ-tube formation by atypical strains of Candida albicans. Antonie Van Leeuwenhoek. 1978;44(1):15–24. doi: 10.1007/BF00400073. [DOI] [PubMed] [Google Scholar]
- Olaiya A. F., Steed J. R., Sogin S. J. Deoxyribonucleic acid-deficient strains of Candida albicans. J Bacteriol. 1980 Mar;141(3):1284–1290. doi: 10.1128/jb.141.3.1284-1290.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riggsby W. S., Torres-Bauza L. J., Wills J. W., Townes T. M. DNA content, kinetic complexity, and the ploidy question in Candida albicans. Mol Cell Biol. 1982 Jul;2(7):853–862. doi: 10.1128/mcb.2.7.853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shepherd M. G., Yin C. Y., Ram S. P., Sullivan P. A. Germ tube induction in Candida albicans. Can J Microbiol. 1980 Jan;26(1):21–26. doi: 10.1139/m80-004. [DOI] [PubMed] [Google Scholar]
- Simonetti N., Strippoli V., Cassone A. Yeast-mycelial conversion induced by N-acetyl-D-glucosamine in Candida albicans. Nature. 1974 Jul 26;250(464):344–346. doi: 10.1038/250344a0. [DOI] [PubMed] [Google Scholar]
- Strippoli V., Simonetti N. Effect of tetracycline on the virulence of Y and M forms of Candida albicans in experimentally induced infections. Mycopathol Mycol Appl. 1973 Sep 28;51(1):65–73. doi: 10.1007/BF02141286. [DOI] [PubMed] [Google Scholar]
- Syverson R. E., Buckley H. R., Campbell C. C. Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans. Infect Immun. 1975 Nov;12(5):1184–1188. doi: 10.1128/iai.12.5.1184-1188.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TASCHDJIAN C. L., BURCHALL J. J., KOZINN P. J. Rapid identification of Candida albicans by filamentation on serum and serum substitutes. AMA J Dis Child. 1960 Feb;99:212–215. doi: 10.1001/archpedi.1960.02070030214011. [DOI] [PubMed] [Google Scholar]
- TASCHDJIAN C. L., KOZINN P. J. Laboratory and clinical studies on candidiasis in the newborn infant. J Pediatr. 1957 Apr;50(4):426–433. doi: 10.1016/s0022-3476(57)80252-2. [DOI] [PubMed] [Google Scholar]