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. 2009 Mar 25;48(3):317–325. doi: 10.1007/s12088-008-0041-2

Functional and safety aspects of enterococci in dairy foods

Arun Bhardwaj 1, R K Malik 1,✉, Prashant Chauhan 1
PMCID: PMC3476767  PMID: 23100728

Abstract

The genus Enterococcus like other LAB has also been featured in dairy industry for decades due to its specific biochemical traits such as lipolysis, proteolysis, and citrate breakdown, hence contributing typical taste and flavor to the dairy foods. Furthermore, the production of bacteriocins by enterococci (enterocins) is well documented. These technological applications have led to propose enterococci as adjunct starters or protective cultures in fermented foods. Moreover, enterococci are nowadays promoted as probiotics, which are claimed for the maintenance of normal intestinal microflora, stimulation of the immune system and improvement of nutritional value of foods. At the same time, enterococci present an emerging pool of opportunistic pathogens for humans as they cause disease, possess agents for antibiotic resistance, and are frequently armed with potential virulence factors. Because of this “dualistic” nature, the use of enterococci remains a debatable issue. However, based on a long history of safe association of particular enterococci with some traditional food fermentations, the use of such strains appears to bear no particular risk for human health. Abundance of knowledge as well as progress in molecular techniques has, however, enabled exact characterization and safety assessment of strains. Therefore, a balanced evaluation of both, beneficial and undesirable nature of enterococci is required. A clear understanding of their status may, therefore, allow their safe use as a starter, or a probiotic strain. The present review describes the broader insight of the benefits and risks of enterococci in dairy foods and their safety assessment.

Keywords: Enterococci, Food safety, Virulence and Functional properties

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References

  • 1.Cintas L.M., Casaus P., Herranz C., Nes I.F., Hernández P.E. Review: bacteriocins of lactic acid bacteria. Int Food Sci Technol. 2001;7:281–305. [Google Scholar]
  • 2.Foulquie Moreno M.R., Sarantinopoulos P., Tsakalidou E., Vuyst L. The role and application of enterococci in food and health. Int J Food Microbiol. 2006;106:1–24. doi: 10.1016/j.ijfoodmicro.2005.06.026. [DOI] [PubMed] [Google Scholar]
  • 3.Cotter P.D., Hill C., Ross R.P. Bacteriocins: developing innate immunity for food. Natu Rev. 2005;3:777–788. doi: 10.1038/nrmicro1273. [DOI] [PubMed] [Google Scholar]
  • 4.Fimland G., Johnsen L., Dalhus B., Nissen-Meyer J. Pediocin-like antimicrobial peptides (class IIa bacteriocins) and their immunity proteins: biosynthesis, structure, and mode of action. J Peptide Sci. 2005;11:688–696. doi: 10.1002/psc.699. [DOI] [PubMed] [Google Scholar]
  • 5.Franz C.M.A.P., Stiles M.E., Schleifer K.H., Holzapfel W.H. Enterococci in foods-a conundrum for food safety. Int J Food Microbiol. 2003;88:105–122. doi: 10.1016/S0168-1605(03)00174-0. [DOI] [PubMed] [Google Scholar]
  • 6.Franz C.M.A.P., Muscholl-Silberhorn A.B., Yousif N.M.K., Vancanneyt M., Swings J., Holzapfel W.H. Incidence of virulence factors and antibiotic resistance among enterococci isolated from food. Appl Environ Microbiol. 2001;67:4385–4389. doi: 10.1128/AEM.67.9.4385-4389.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Pillar C.M., Gilmore M.S. Enterococcal virulence — pathogenicity island of Enterococcus faecalis. Front Biosci. 2004;9:2335–2346. doi: 10.2741/1400. [DOI] [PubMed] [Google Scholar]
  • 8.Kayser F.H. Safety aspects of enterococci from the medical point of view. Int J Food Microbiol. 2003;88:255–262. doi: 10.1016/S0168-1605(03)00188-0. [DOI] [PubMed] [Google Scholar]
  • 9.Nallapareddy N., Wenxiang H., Weinstock G.M., Murray B. Molecular characterization of a widespread, pathogenic, and antibiotic resistance receptive Enterococcus faecalis lineage and dissemination of its putative pathogenicity island. J Bacteriol. 2005;187:5709–5718. doi: 10.1128/JB.187.16.5709-5718.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 10.Dargere S., Vergnaud M., Verdon R., Saloux E., Page O., Leclercq R., Bazin C. Enterococcus gallinarum endocarditis occurring on native heart valves. J Clin Microbiol. 2002;40:2308–2310. doi: 10.1128/JCM.40.6.2308-2310.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Poyart C., Lambert T., Morand P., Abassade P., Quesne G., Baudouy Y., Trieu-Cuot P. Native valve endocarditis due to Enterococcus hirae. J Clin Microbiol. 2002;40:2689–2690. doi: 10.1128/JCM.40.7.2689-2690.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 12.Higashide T., Takahashi M., Kobayashi A., Ohkubo S., Sakurai M., Shirao Y., Tamura T., Sugiyama K. Endophtalmitis caused by Enterococcus mundtii. J Clin Microbiol. 2005;43:1475–1476. doi: 10.1128/JCM.43.3.1475-1476.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 13.Eaton T.J., Gasson M.J. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol. 2001;67:1628–1635. doi: 10.1128/AEM.67.4.1628-1635.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 14.Semedo T., Almeida Santos M., Silva Lopes M.F., Figueiredo Marqués J.J., Barreto Crespo M.T., Tenreiro R. Virulence factors in food, clinical and reference enterococci: a common trait in the genus? Syst Appl Microbiol. 2003;26:13–22. doi: 10.1078/072320203322337263. [DOI] [PubMed] [Google Scholar]
  • 15.Martín M., Gutiérrez J., Criado R., Herranz C., Cintas L.M., Hernández P.E. Genes encoding bacteriocins and their expression and potential virulence factors of enterococci isolated from wood pigeons (Columba palumbus) J Food Prot. 2006;69:520–531. doi: 10.4315/0362-028x-69.3.520. [DOI] [PubMed] [Google Scholar]
  • 16.Cocconcelli P.S., Cattivelli D., Gazzola S. Gene transfer of vancomycin and tetracycline resistances among Enterococcus faecalis during cheese and sausage fermentations. Int J Food Microbiol. 2003;88(2–3):315–323. doi: 10.1016/S0168-1605(03)00194-6. [DOI] [PubMed] [Google Scholar]
  • 17.Saavedra L., Taranto M.P., Sesma F., Valdez G.F. Homemade traditional cheeses for the isolation of probiotic Enterococcus faecium strains. Int J Food Microbiol. 2003;88:241–245. doi: 10.1016/S0168-1605(03)00186-7. [DOI] [PubMed] [Google Scholar]
  • 18.Devriese L.A., Pot B. In: The Lactic Acid Bacteria, the genera of lactic acid bacteria, the genus Enterococcus. Wood B.J.B., Holzapfel W.H., editors. London, United Kingdom: Blackei Academic and Professional; 1995. pp. 327–367. [Google Scholar]
  • 19.Lancefield R.C. A serological differentiation of human and other groups of hemolytic streptococci. J Exp Med. 1933;57:571–595. doi: 10.1084/jem.57.4.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 20.Sherman J.M. The streptococci. Bacteriol Rev. 1937;1:3–97. doi: 10.1128/br.1.1.3-97.1937. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 21.Devriese L.A., Pot B., Collins M.D. Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol. 1993;75:399–408. doi: 10.1111/j.1365-2672.1993.tb02794.x. [DOI] [PubMed] [Google Scholar]
  • 22.Stiles M (2002) Safety aspects of enterococci from food point of view. In: Symposium on Enterococci in Foods-Functional and Safety aspects, Berlin, Abstract Book.
  • 23.Schleifer K.H., Kilpper-Bälz R. Transfer of Streptococcus faecalis and Streptococcus faecium to the genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov. Int J Syst Bacteriol. 1984;34:31–34. doi: 10.1099/00207713-34-1-31. [DOI] [Google Scholar]
  • 24.Hartman P.A., Deibel R.H., Sieverding L.M. Enterococci. In: Downes F.P., Ito K., editors. Compendium of methods for the microbiological examination of foods. Washington, DC, USA: Amer Public Health Assoc; 2001. pp. 83–87. [Google Scholar]
  • 25.Mundt OJ (1986) Enterococci. In: Bergey’s Manual of Systematic Bacteriology, vol 2 (Sneath PHA, Mair NS, Sharpe ME and Holt JG eds). Williams, Wilkins and Baltimore pp. 1063–1065
  • 26.Giraffa G. Functionality of enterococci in dairy products. Int J Food Microbiol. 2003;88:215–222. doi: 10.1016/S0168-1605(03)00183-1. [DOI] [PubMed] [Google Scholar]
  • 27.Hill BM and Smythe BW (1997) A study of the microbiological quality of raw milk from individual farms. New Zealand Dairy Res Inst 1–35
  • 28.Franz C.M.A.P., Holzapfel W.H., Stiles M.E. Enterococci at the crossroads of the food safety? Int J Food Microbiol. 1999;47:1–24. doi: 10.1016/S0168-1605(99)00007-0. [DOI] [PubMed] [Google Scholar]
  • 29.Gelsomino R., Vancanneyt M., Condon S., Swings J., Cogan T.M. Enterococcal diversity in the environment of an Irish Cheddar-type cheese making factory. Int J Food Microbiol. 2001;71:177–188. doi: 10.1016/S0168-1605(01)00620-1. [DOI] [PubMed] [Google Scholar]
  • 30.Perez B.S., Lorenzo P.L., Garcia M.L., Hernandez P.E., Ordonez J.A. Heat-Resistance of Enterococci. Milchwissenschaft-Milk Sci Int. 1982;37(12):724–726. [Google Scholar]
  • 31.Anonymous (1992) EEC council directive of 16 June 1992 on milk hygiene (92/46EEC). Off J Euro Commu L268/1.
  • 32.Birollo G.A., Reinheimer J.A., Vinderola C.G. Enterococci vs. nonlactic acid microflora as hygiene indicators for sweetened yoghurt. Food Microbiol. 2001;18:597–604. doi: 10.1006/fmic.2001.0435. [DOI] [Google Scholar]
  • 33.Sarantinopoulos P., Kalantzopoulos G., Tsakalidou E. Effect of Enterococcus faecium on microbiological, physicochemical and sensory characteristics of Greek Feta cheese. Int J Food Microbiol. 2002;76:93–105. doi: 10.1016/S0168-1605(02)00021-1. [DOI] [PubMed] [Google Scholar]
  • 34.Manolopoulou E., Sarantinopoulos P., Zoidou E., Aktypis A., Moschopoulou E., Kandarakis I.G., Anifantakis E.M. Evolution of microbial populations during traditional Feta cheese manufacture and ripening. Int J Food Microbiol. 2003;82(2):153–161. doi: 10.1016/S0168-1605(02)00258-1. [DOI] [PubMed] [Google Scholar]
  • 35.Gelsomino R., Vancanneyt M., Cogan T.M., Condon S., Swings J. Source of enterococci in a farmhouse raw-milk cheese. Appl Environ Microbiol. 2002;68:3560–3565. doi: 10.1128/AEM.68.7.3560-3565.2002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 36.Andrighetto C., Knijff E., Lombardi A., Torriani S., Vancanneyt M., Kersters K., Swings J., Dellaglio F. Phenotypic and genetic diversity of enetrococci isolated from Italian cheeses. J Dairy Res. 2001;68:303–316. doi: 10.1017/S0022029901004800. [DOI] [PubMed] [Google Scholar]
  • 37.Čanžek Majhenič A., Rogelj I., Perko B. Enterococci from Tolminc cheese: population structure, antibiotic susceptibility and incidence of virulence determinants. Int J Food Microbiol. 2005;102:239–244. doi: 10.1016/j.ijfoodmicro.2004.12.021. [DOI] [PubMed] [Google Scholar]
  • 38.Coppola S., Parente E., Dumontet S., Lapeccerella A. The Microflora of Natural Whey Cultures Utilized as Starters in the Manufacture of Mozzarella Cheese from Water-Buffalo Milk. Lait. 1998;68(3):295–309. doi: 10.1051/lait:1988319. [DOI] [Google Scholar]
  • 39.Freitas A.C., Pais C., Malcata F.X., Hogg T.A. Microbiological characterization of Picante de Beira Baixa cheese. J Food Prot. 1995;59:155–160. doi: 10.4315/0362-028X-59.2.155. [DOI] [PubMed] [Google Scholar]
  • 40.Centeno J.A., Mene’ndez S., Rodriguez-Otero J.L. Main microbial flora present as natural starters in Cebreiro raw cow’s-milk cheese (Northwest Spain) Int J Food Microbiol. 1996;33:307–313. doi: 10.1016/0168-1605(96)01165-8. [DOI] [PubMed] [Google Scholar]
  • 41.Mene’ndez S., Godý’nez R., Centeno J.A., Rodrý’guez-Otero J.L. Microbiological, chemical and biochemical characteristics of “Tetilla” raw cows-milk cheese. Food Microbiol. 2001;18:151–158. doi: 10.1006/fmic.2000.0385. [DOI] [Google Scholar]
  • 42.Mannu L., Paba A. Genetic diversity of lactococci and enterococci isolated from home-made Pecorino Sardo ewes’ milk cheese. J Appl Microbiol. 2002;92:55–62. doi: 10.1046/j.1365-2672.2002.01489.x. [DOI] [PubMed] [Google Scholar]
  • 43.Serio A., Paparalla A., Chaves-Lopez C., Corsetti A., Suzzi G. Enterococcus populations in Pecorino Abruzzese cheese: biodiversity and safety aspects. J Food Prot. 2007;70(7):1561–1568. doi: 10.4315/0362-028x-70.7.1561. [DOI] [PubMed] [Google Scholar]
  • 44.Templer S.P., Baumgartner A. Enterococci from appenzeller and schabziger raw milk cheese: antibiotic resistance, virulence factors, and persistence of particular strains in the products. J Food Prot. 2007;70(2):450–455. doi: 10.4315/0362-028x-70.2.450. [DOI] [PubMed] [Google Scholar]
  • 45.Litopolou-Tzanetaki E. Changes in numbers and kinds of lactic acid bacteria during ripening of Kefalotyri chees. J Food Sci. 1990;55:111–113. doi: 10.1111/j.1365-2621.1990.tb06029.x. [DOI] [Google Scholar]
  • 46.Macedo C.A., Malcata F.X., Hogg T.A. Microbiological profile in Serra ewe’s cheese during ripening. J Appl Bacteriol. 1995;79:1–11. [Google Scholar]
  • 47.Sarantinopoulos P., Andrighetto C., Georgalaki M.D., Rea M.C., Lombardi A., Cogan T.M., Kalantzopoulos G., Tsakalidou E. Biochemical properties of enterococci relevant to their technological performance. Int Dairy J. 2001;11:621–647. doi: 10.1016/S0958-6946(01)00087-5. [DOI] [Google Scholar]
  • 48.Lo’pez-Diaz T.M., Santos J.A., Gonzalez C.J., Moreno B., Garcia M.L. Bacteriological quality of traditional Spanish blue cheese. Milchwissenschaft. 1995;50:503–505. [Google Scholar]
  • 49.Jensen J.P., Reinbold G.W., Washam C.J., Vedamuthu E.R. Role of enterococci in Cheddar cheese: free fatty acid appearance and citric acid utilization. J Milk Food Technol. 1975;38:78–83. [Google Scholar]
  • 50.Jensen J.P., Reinbold G.W., Washam C.J., Vedamuthu E.R. Role of enterococci in Cheddar cheese: proteolytic activity and lactic acid development. J Milk Food Technol. 1975;38:3–7. [Google Scholar]
  • 51.Litopoulou-Tzanetaki E., Tzanetakis N., Vafopouloumastrojiannaki A. Effect of the Type of Lactic Starter on Microbiological Chemical and Sensory Characteristics of Feta Cheese. Food Microbiol. 1993;10(1):31–41. doi: 10.1006/fmic.1993.1004. [DOI] [Google Scholar]
  • 52.Villani F., Coppola S. Selection of enterococcal strains for water-buffalo Mozzarella cheese manufacture. Annali di Microbiologia ed Enzimologia. 1994;44:97–105. [Google Scholar]
  • 53.Centeno J.A., Menendez S., Hermida M.A., Rodrý’guez-Otero J.L. Effects of the addition of Enterococcus faecalis in Cebreiro cheese manufacture. Int J Food Microbiol. 1999;48:97–111. doi: 10.1016/S0168-1605(99)00030-6. [DOI] [PubMed] [Google Scholar]
  • 54.Menendez S., Godinez R., Hermida M., Centeno J.A., Rodriguez-Otero J.L. Characteristics of “Tetilla” pasteurised milk cheese manufactured with the addition of autochthonous cultures. Food Microbiol. 2004;21:97–104. doi: 10.1016/S0740-0020(03)00014-5. [DOI] [Google Scholar]
  • 55.ACNFP (1996) Report on Enterococcus faecium, strain K77D. In: MAFF Advisory Committee on Novel Foods and Processes, Report, Ergon House c/o Nobel House, 17 Smith Square, London SW1 3JR, United Kingdom
  • 56.Leroy F., Foulquie Moreno M.R., Vuyst L. Enterococcus faecium RZS C5, an interesting bacteriocin producer to be used as a co-culture in food fermentation. Int J Food Microbiol. 2003;88(2–3):235–240. doi: 10.1016/S0168-1605(03)00185-5. [DOI] [PubMed] [Google Scholar]
  • 57.Belkum M.J., Stiles M.E. Nonlantibiotic antibacterial peptides from lactic acid bacteria. Nat Pro Rep. 2000;17:323–335. doi: 10.1039/a801347k. [DOI] [PubMed] [Google Scholar]
  • 58.Giraffa G. Enterococcal bacteriocins: their potential as anti-Listeria factors in dairy technology. Food Microbiol. 1995;12:291–299. doi: 10.1016/S0740-0020(95)80109-X. [DOI] [Google Scholar]
  • 59.Maisnier-Patin S., Forni E., Richard J. Purification, partial characterization and mode of action of enterococcin EFS2, an antilisterial bacteriocins produced by a strain of Enterococcus faecalis isolated from a cheese. Int J Food Microbiol. 1996;30:255–270. doi: 10.1016/0168-1605(96)00950-6. [DOI] [PubMed] [Google Scholar]
  • 60.Cintas L.M., Casaus P., Fena’ndez M., Herna’ndez P.E. Comparative antimicrobial activity of enterocin L50, pediocin PA-1, nisin A and lactocin S against spoilage and foodborne pathogenic bacteria. Food Microbio. 1998;l15:289–298. doi: 10.1006/fmic.1997.0160. [DOI] [Google Scholar]
  • 61.Mohar P, Čanžek Majhenič A and Rogelj I (2005) Phenotypic characterisation, antibiotic susceptibility and antimicrobial activity of enterococcal population from Karst ewe’s cheese. In: 2nd Int ASM-FEMS Conference on Enterococci, Helsingor, Denmark (poster)
  • 62.Ga’lvez A., Gime’nez-Gallego G., Maqueda M., Valdivia E. Purification and amino acid composition of peptide antibiotic AS-48 produced by Streptococcus (Enterococcus) faecalis subsp. liquefaciens S-48. Antimicro Agents Chemo. 1989;33:437–441. doi: 10.1128/aac.33.4.437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 63.Tomita H., Fujimoto S., Tanimoto K., Ike Y. Cloning and genetic organization of the bacteriocin 21 determinant encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pPD1. J Bacteriol. 1997;179:7843–7855. doi: 10.1128/jb.179.24.7843-7855.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 64.Simonetta A.C., Moragues de Velasco L.G., Friso’n L.N. Antibacterial activity of enterococci strains against Vibrio cholerae. Lett Appl Microbiol. 1997;24:139–143. doi: 10.1046/j.1472-765X.1997.00370.x. [DOI] [PubMed] [Google Scholar]
  • 65.Čanžek Majhenič A. Enterococci: yin-yang microbes. Mljekarstvo. 2006;56(1):5–20. [Google Scholar]
  • 66.Franz C.M.A.P., Belkum M.J., Holzapfel W.H., Abrioul H., Galvez A. Diversity of enterococcal bacteriocins and their grouping in a new classification scheme. FEMS Microbiol Rev. 2007;31:293–310. doi: 10.1111/j.1574-6976.2007.00064.x. [DOI] [PubMed] [Google Scholar]
  • 67.Tannock G.W., Cook G. Enterococci as members of the intestinal microflora of humans. In: Gilmore M.S., editor. The enterococci: pathogenesis, molecular biology, and antibiotic resistance. Washington: ASM Press; 2002. p. 101. [Google Scholar]
  • 68.Wunderlich P.F., Braun L., Fumagalli I., D’Apuzzo V., Heim F., Karly M., Lodi R., Politta G., Vonbank F., Ja Zeltner L. Double-blind report on the efficacy of lactic acid-producing Enterococcus SF68 in the prevention of antibiotic-associated diarrhoea and in the treatment of acute diarrhoea. J Int Medi Res. 1989;17:333–338. doi: 10.1177/030006058901700405. [DOI] [PubMed] [Google Scholar]
  • 69.Bellomo G., Mangiagle A., Nicastro L., Frigerio G. A controlled double-blind study of SF68 strain as a new biological preparation for the treatment of diarrhoea in pediatrics. Curr Therap Res. 1980;28:927–936. [Google Scholar]
  • 70.Buydens P., Debeuckelaere S. Efficacy of SF 68 in the treatment of acute diarrhea. A placebo-controlled trial. Sc J Gastroenterol. 1996;31:887–891. doi: 10.3109/00365529609051997. [DOI] [PubMed] [Google Scholar]
  • 71.Benyacoub J., Perez P.F., Rochat F., Saudan K.Y., Reuteler G., Antille N., Humen M., Antoni G.L., Cavadini C., Blum S., Schiffrin E.J. Enterococcus faecium SF68 enhances the immune response to Giardia intestinalis in mice. J Nutr. 2005;135:1171–1176. doi: 10.1093/jn/135.5.1171. [DOI] [PubMed] [Google Scholar]
  • 72.Benyacoub J., Czarnecki-Maulden G.L., Cavadini C., Sauthier T., Anderson R.E., Schiffrin E.J., Weid T. Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs. J Nut. 2003;133:1158–1162. doi: 10.1093/jn/133.4.1158. [DOI] [PubMed] [Google Scholar]
  • 73.Agerholm-Larsen L., Bell M.L., Grunwald G.K., Astrup A. The effect of a probiotic milk product on plasma cholesterol: a meta-analysis of short-term intervention studies. Euro J Clin Nutr. 2000;54(11):856–860. doi: 10.1038/sj.ejcn.1601104. [DOI] [PubMed] [Google Scholar]
  • 74.Richelsen B., Kristensen K., Pedersen S.B. Longterm (6 months) effect of a new fermented milk product on the level of plasma lipoproteins — A placebo-controlled and double blind study. Euro J Clin Nut. 1996;50(12):811–815. [PubMed] [Google Scholar]
  • 75.Sessions V.A., Lovegrove J.A., Taylor G.R.J. The effect of a new fermented milk product on total plasma cholesterol, LDL-cholesterol and apolipoprotein B concentrations in middle-aged men and women. In: Sadler M.J., Saltmarch M., editors. Functional Foods: The Consumer, The Product, and The Evidence. London, United Kingdom: The Royal Society of Chemistry; 1997. pp. 15–19. [Google Scholar]
  • 76.Hilvak P., Odraska J., Ferencik M., Ebringer L., Jahnova E., Mikes Z. One-year application of probiotic strain Enterococcus faecium M-74 decreases serum cholesterol levels. Bratisl Lek Listy. 2005;106(2):67–72. [PubMed] [Google Scholar]
  • 77.Rossi E.A., Vendramini R.C., Carlos I.Z., Pei Y.C., Valdez G.F. Development of a novel fermented soymilk product with potential probiotic properties. Euro Food Res Technol. 1999;209:305–307. doi: 10.1007/s002170050499. [DOI] [Google Scholar]
  • 78.Allen WD, Linggood MA and Porter P (1996) Enterococcus organisms and their use as probiotics in alleviating irritable bowel syndrome symptoms. Euro Patent 0508701 (B1)
  • 79.Gardiner G.E., Ross R.P., Wallace J.M., Scanlan F.P., Jagers P., Fitzgerald G.F., Collins J.K., Stanton C. Influence of a probiotic adjunct culture of Enterococcus faecium on the quality of cheddar cheese. J Agri Food Chem. 1999;47(12):4907–4916. doi: 10.1021/jf990277m. [DOI] [PubMed] [Google Scholar]
  • 80.European Commission (2004) List of the authorized additives in feeding stuffs published in application of Article 9t (b) of Council Directive 70/524/EEC concerning additives in feeding stuffs. Official J Euro Union C50
  • 81.Giraffa G. Enterococci from foods. FEMS Microbiol Rev. 2002;26:163–171. doi: 10.1111/j.1574-6976.2002.tb00608.x. [DOI] [PubMed] [Google Scholar]
  • 82.Karovacova J., Kohajdova Z. Biogenic Amines in food. Chem Pap. 2005;59(1):70–79. [Google Scholar]
  • 83.Martuscelli M., Gardini F., Torriani S., Mastrocola D., Serio A., Chaves-Lopez C., Schirone M., Suzzi G. Production of biogenic amines during the ripening of Pecorino Abruzzese cheese. Int Dairy J. 2005;15:571–578. doi: 10.1016/j.idairyj.2004.11.008. [DOI] [Google Scholar]
  • 84.Bover-cid S., Hugas M., Izquierdo-Pulido M., Vidal-Carou M.C. Amino acid-decarboxylase activity of bacteria isolated from fermented pork sausages. Int J Food Microbiol. 2001;66:185–189. doi: 10.1016/S0168-1605(00)00526-2. [DOI] [PubMed] [Google Scholar]
  • 85.Clewell D.B. Bacterial sex pheromone-induced plasmid transfer. Cell. 1993;73:9–12. doi: 10.1016/0092-8674(93)90153-H. [DOI] [PubMed] [Google Scholar]
  • 86.Dunny G.M., Leonard B.A.B., Hedberg P.J. Pheromone-Inducible Conjugation in Enterococcus faecalis: Interbacterial and Host-Parasite Chemical Communication. J Bacteriol. 1995;177(4):871–876. doi: 10.1128/jb.177.4.871-876.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 87.Johnson A.P. The Pathogenicity of Enterococci. J Antimicro Chemo. 1994;33(6):1083–1089. doi: 10.1093/jac/33.6.1083. [DOI] [PubMed] [Google Scholar]
  • 88.Witte W., Wirth R., Klare I. Enterococci. Chemo. 1999;45:135–145. doi: 10.1159/000007174. [DOI] [PubMed] [Google Scholar]
  • 89.Su Y.A., Sulavik M.C., He P., Mäkinen K.K., Mäkinen P., Fiedler S., Wirth R., Clewell D.B. Nucleotide sequence of the gelatinase gene (gelE) from Enterococcus faecalis subsp. liquefaciens. Infect Immun. 1991;59:415–420. doi: 10.1128/iai.59.1.415-420.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 90.Qin X., Singh K.V., Weinstock G.M., Murray B.E. Effects of Enterococcus faecalis fsr genes on production of gelatinase and a serine protease and virulence. Infect Immun. 2000;68:2579–2586. doi: 10.1128/IAI.68.5.2579-2586.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 91.Mannu L., Paba A., Daga E., Comunian R., Zanetti S., Dupre I., Sechi L.A. Comparison of the incidence of virulence determinants and antibiotic resistance between Enterococcus faecium strains of dairy, animal and clinical origin. Int J Food Microbiol. 2003;88:291–304. doi: 10.1016/S0168-1605(03)00191-0. [DOI] [PubMed] [Google Scholar]
  • 92.Klare I., Konstabel C., Badstubner D., Werner G., Witte W. Occurrence and spread of antibiotic resistances in Enterococcus faecium. Int J Food Microbiol. 2003;88:269–290. doi: 10.1016/S0168-1605(03)00190-9. [DOI] [PubMed] [Google Scholar]
  • 93.Yousif N.M.K., Dawyndt P., Abriouel H., Wijaya A., Schillinger U., Vancanneyt M., Swings J. Molecular characterization, techonological properties and safety aspects of enterococci from “Hussuwa”, an African fermented sorghum products. J Appl Microbiol. 2005;98:216–228. doi: 10.1111/j.1365-2672.2004.02450.x. [DOI] [PubMed] [Google Scholar]
  • 94.Perez-Pulido R., Abriouel H., Ben Omer N., Lucas R., Martinez-Canamero M., Galvez A. Safety and potential risks of enterococci isolated from traditional fermented capers. Food Chem Toxicol. 2006;44:2070–2077. doi: 10.1016/j.fct.2006.07.008. [DOI] [PubMed] [Google Scholar]
  • 95.Omar B.N., Castro A., Lucas R., Abriouel H., Yousif N.M.K., Franz C.A.M.P., Holzapfel W.H., Perez-Pulido R., Martinez-Canamero M., Galvez A. Functional and safety aspects of enterococci isolated from different Spanish foods. Syst Appl Microbiol. 2004;27:118–130. doi: 10.1078/0723-2020-00248. [DOI] [PubMed] [Google Scholar]
  • 96.Lopes M.F.S., Simões A.P., Tenreiro R., Marques J.J.F., Crespo M.T.B. Activity and expression of a virulence factor, gelatinase, in dairy enterococci. Int J Food Microbiol. 2006;112:208–214. doi: 10.1016/j.ijfoodmicro.2006.09.004. [DOI] [PubMed] [Google Scholar]
  • 97.Sánchez J., Basanta A., Gómez-Sala B., Herranz C., Cintas L.M., Hernández P.E. Antimicrobial and safety aspects and biotechnological potential of bacteriocinogenic enterococci isolated from mallard ducks (Anas platyrhynchos) Int J Food Microbiol. 2007;117:295–305. doi: 10.1016/j.ijfoodmicro.2007.04.012. [DOI] [PubMed] [Google Scholar]
  • 98.Hufnagel M., Koch S., Kropec A., Huebner J. Opsonophagocytic assay as a potentially useful tool for assessing safety of enterococcal preparations. Int J Food Microbiol. 2003;88:263–267. doi: 10.1016/S0168-1605(03)00189-2. [DOI] [PubMed] [Google Scholar]
  • 99.Kropec A., Hufnagel M., Zimmermann K., Huebner J. In vitro assessment of the host response against Enterococcus faecalis used in probiotic preparations. Infect. 2005;33(5/6):377–379. doi: 10.1007/s15010-005-5063-7. [DOI] [PubMed] [Google Scholar]

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