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. 2009 Mar 25;48(3):365–371. doi: 10.1007/s12088-008-0045-y

Characterization of carbonic anhydrase from diversified genus for biomimetic carbon-dioxide sequestration

Anjana Sharma 1,✉, Abhishek Bhattacharya 1, Rajesh Pujari 1, Ankita Shrivastava 1
PMCID: PMC3476768  PMID: 23100736

Abstract

Diversified group of bacteria were screened for carbonic anhydrase (CA) activity. Significant CA activity was found in crude enzyme extracts of Enterobacter and Aeromonas isolates while minimal or negligible CA activity was observed in case of Shigella and Klebsiella spp. Optimization and characterization study of potent CA producing isolates revealed that the maximum enzyme activity of 3.86 EU/ml was observed in E. taylorae and the optimum pH range for enzyme stability was found to be 7.5–9.0 along with an optimum temperature range of 35–50 °C. The molecular mass of CA was 29-kDa indicating α-type with periplasmic and cytosolic location. Present investigation for the first time reports CA in diversified genus and optimized parameters for enhanced production of CA in Enterobacter sp. & Aeromonas sp. from fresh water bodies that inturn lay down grounds for exploitation of CA from E. taylorae as an efficient catalyst for CO2 sequestration within a bioreactor.

Keywords: Carbonic anhydrase, Characterization, CO2 sequestration, E. taylorae, Optimization

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References

  • 1.Kupriyanova E., Villarejo A., Markelova A., Gerasimenko L., Zavarzin G., Samuelsson G., Los D.A., Pronina N. Extracellular Carbonic anhydrases of stromatolite forming cyanobacterium Microcoleus cathonoplastes. Microbiology. 2007;153:1149–156. doi: 10.1099/mic.0.2006/003905-0. [DOI] [PubMed] [Google Scholar]
  • 2.Bond G.M., Stranger J., Branchvold D.K., Sinsek F.A., Medina N.G., Egeland G. Development of integrated system for biomimetic CO2 Sequestration using the enzyme carbonic anhydrase. Energy & Fuels. 1999;15:309–31. doi: 10.1021/ef000246p. [DOI] [Google Scholar]
  • 3.Smith K.S., Ferry J.G. Prokaryotic Carbonic anhydrases. FEMS Microbiol Rev. 2000;24:335–366. doi: 10.1111/j.1574-6976.2000.tb00546.x. [DOI] [PubMed] [Google Scholar]
  • 4.Adler L., Brundell J., Falkbring S.O., Nyman P.O. Carbonic anhydrase from Nisseria sicca strain 6021. Bacterial Growth and Purification of enzyme. Biochemica et Biophysica Acta. 1972;284:313–323. doi: 10.1016/0005-2744(72)90068-x. [DOI] [PubMed] [Google Scholar]
  • 5.Alber B.E., Ferry J.G. A carbonic anhydrase from the archaeon Methanosarcina thermophila. Proc Natl Acad Sci USA. 1994;91:6909–6913. doi: 10.1073/pnas.91.15.6909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 6.Braus-Stromeyer S., Schnappauf G., Braus G.H., Gobner A., Drake H.L. Carbonic anhydrase in Acetobacterium woodii. J Bacteriol. 1997;179:7197–7200. doi: 10.1128/jb.179.22.7197-7200.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 7.Gill S.R., Fedorka P.J., Tweten R.K., Sleeper B.P. Purification and Properties of Carbonic anhydrase of Rhodospirullum rubrum. Arch Microbiol. 1984;138:113–118. doi: 10.1007/BF00413010. [DOI] [Google Scholar]
  • 8.Yagawa Y., Shiraiwa Y., Miyachi S. Carbonic anhydrase from the blue green algae (cyanobacterium) Anabaena variabilis. Plant Cell Physiol. 1984;25:775–783. [Google Scholar]
  • 9.Buzolyova L.S., Somov G. Autotrophic assimilation of CO2 and C1 compounds by pathogenic bacteria. Biochem (Moscow) 1999;64(10):1357–1361. [PubMed] [Google Scholar]
  • 10.Smith K.S., Zakubzick C., Whittam T.S., Ferry J.G. Carbonic anhydrase is an ancient enzyme wide spread in prokaryotes. Proc Natl Acad Sci USA. 1999;96:15184–15189. doi: 10.1073/pnas.96.26.15184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 11.Dean C.R., Ward O.P. The use of EDTA or polymixin with lysozyme for the recovery of intracellular products from Eschericia coli. Biotechnology Techniques. 1992;6(2):133–138. doi: 10.1007/BF02438819. [DOI] [Google Scholar]
  • 12.Wilbur K.M., Anderson N.G. Electrometric and Colorimetric determination of Carbonic anhydrase. J Biolchem. 1948;176:147–154. [PubMed] [Google Scholar]
  • 13.Lowry O.H., Rosenbrough N.J., Forr A.L., Randall R.J. Protein measurement with Folin Phenol Reagent. J Biolchem. 1951;193:265–275. [PubMed] [Google Scholar]
  • 14.Smith K.S., Ferry J.G. A plant type (β-Class) carbonic anhydrase in the thermophilic methanoarchaeon Methanobacterium thermoautotrophicum. J Bacteriol. 1999;181:6247–6253. doi: 10.1128/jb.181.20.6247-6253.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 15.Ames G., Prody F.L., Kustu S. Simple, rapid and quantitative release of periplasmic proteins by choloroform. J Bacteriol. 1984;160:1181–1183. doi: 10.1128/jb.160.3.1181-1183.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 16.Russel M., Model P. Filamentous phage precoat is an integral membrane protein: analysis by a new method of membrane preparation. Cell. 1982;28:177–184. doi: 10.1016/0092-8674(82)90387-7. [DOI] [PubMed] [Google Scholar]
  • 17.Laemmli U.K. Cleavage of structural proteins during the assembly of head of bacteriophage T4. Nature (London) 1970;227:680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  • 18.Merlin C., Masters M., Mcteer S., Corlson A. Why is carbonic anhydrase essential to E. coli. J Bacteriol. 2003;185(21):6415–6424. doi: 10.1128/JB.185.21.6415-6424.2003. [DOI] [PMC free article] [PubMed] [Google Scholar]
  • 19.Charles H.P., Roberts Carbon dioxide as a growth factor for mutants of E. coli. J Gen Microbiol. 1968;51:211–224. doi: 10.1099/00221287-51-2-211. [DOI] [PubMed] [Google Scholar]
  • 20.Demir N., Demir Y., Coskum P. Purification and characterization of carbonic anhydrase from human erythrocyte plasma membrane. Turk J Med Sci. 2001;31:477–482. [Google Scholar]
  • 21.Demir Y., Demir N., Nadarglu H., Bakan E. Purification and characterization of carbonic anhydrase from bovine erythrocyte plasma membrane. Biochem & Biotechnol. 2000;30(1):49–59. doi: 10.1080/10826060008544944. [DOI] [PubMed] [Google Scholar]
  • 22.Stahler M.F., Ganter L., Katherin L., Manfred K., Stephen B. Mutational analysis of Helicobacter pylori carbonic anhydrases. FEMS Immunol Medical Microbiol. 2005;44:183–189. doi: 10.1016/j.femsim.2004.10.021. [DOI] [PubMed] [Google Scholar]
  • 23.Park I.S., Hausinger R.P. Requirement of carbon dioxide for in vitro assembly of urease nickel metallocenter. Sci. 1995;267:1156–1158. doi: 10.1126/science.7855593. [DOI] [PubMed] [Google Scholar]
  • 24.Earnhardt J.N., Qian M.C., Lakkis M.M., Bergenhem N.C., Laipis P.Y., Tashian R.E., Silverman D.M. The catalytic properties of marine carbonic anhydrase VII. Biochem. 1998;37:10837–10845. doi: 10.1021/bi980046t. [DOI] [PubMed] [Google Scholar]
  • 25.Chirica L.C., Elleby B., Jonsson B.H., Lindskog S. The complete sequence, expression in E. coli, purification and some properties of carbonic anhydrase from Nisseria gonorrhoeae. Eur J Biochem. 1997;244:755–760. doi: 10.1111/j.1432-1033.1997.00755.x. [DOI] [PubMed] [Google Scholar]
  • 26.Chirica L., Peteresson C., Hurtig M., Jonsson B.H., Boren T., Lindskog S. Expression and localization of α and β Carbonic anhydrase in Helicobacter pylori. Biochemica and Biophysica Acta. 2002;160:192–199. doi: 10.1016/s1570-9639(02)00467-3. [DOI] [PubMed] [Google Scholar]
  • 27.Li W., Yu L.J., Wu Y., Jia L.P., Yuan D.X. Enhancement of calcium release from limestone by microbial extracellular carbonic anhydrase. Bioresource Tech. 2007;98:950–953. doi: 10.1016/j.biortech.2006.03.021. [DOI] [PubMed] [Google Scholar]
  • 28.Pushkas L.G., Inui M., Zahan K., Yukawa H. A periplasmic α-type carbonic anhydrase from Rhodopseudomonas palustris is essential for bicarbonate uptake. Microbiology. 2000;146:2957–2966. doi: 10.1099/00221287-146-11-2957. [DOI] [PubMed] [Google Scholar]
  • 29.Rawat M., Moroney J.V. The regulation of carbonic anhydrase and ribulose 1,5 biphosphate carboxylase / Oxygenase activase by light and CO2 in Chlamydomonas reinhardtii. Plant Physiol. 1995;109:937–944. doi: 10.1104/pp.109.3.937. [DOI] [PMC free article] [PubMed] [Google Scholar]

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