Skip to main content
PMC home page
Indian Journal of Microbiology logoLink to Indian Journal of Microbiology
. 2008 Jun 13;48(3):326–330. doi: 10.1007/s12088-008-0023-4

Preparation of FMD type A87/IRN inactivated vaccine by gamma irradiation and the immune response on guinea pig

Farahnaz Motamedi Sedeh 1, Akbar Khorasani 2, Kamal Shafaee 1, Hadi Fatolahi 1, Kourosh Arbabi 1
PMCID: PMC3476770  PMID: 23100729

Abstract

FMD is one of the most economically damaging diseases that affect livestock animals. In this study FMD Virus type A87/IRN was multiplied on BHK21 cells. The virus was titrated by TCID50 method, it was 107.5/ml. The FMD virus samples were inactivated by gamma ray from 60Co source at −20°C. Safety test was done by IBRS2 monolayer cell culture method, also antigenicity of irradiated and un-irradiated virus samples were studied by Complement Fixation Test. The Dose/Survival curve for irradiated FMD Virus was drawn, the optimum dose range for inactivation of FMDV type A87/IRN and unaltered antigenicity was obtained 40–44 kGy. The inactivated virus samples by irradiation and ethyleneimine (EI) were formulated respectively as vaccine with Al(OH)3 gel and other substances. The vaccines were inoculated to Guinea pigs and the results of Serum Neutralization Test for the normal vaccine and radio-vaccine showed protective titer after 8 months. The potency test of the inactivated vaccines was done, PD50 Value of the vaccines were calculated 7.06 and 5.6 for inactivated vaccine by EI and gamma irradiation respectively.

Keywords: Vaccine, Foot and Mouth Disease, Virus, gamma irradiation

Full Text

The Full Text of this article is available as a PDF (641.1 KB).

References

  • 1.Zhidong Z., Soren A. Quantitative analysis of foot and mouth disease virus RNA loads in bovine tissues. J Gene Virol. 2004;85:2567–2575. doi: 10.1099/vir.0.80011-0. [DOI] [PubMed] [Google Scholar]
  • 2.Barteling S.J., Vreeswijk J. Developments in FMD vaccines. Vaccine. 1991;9:75–87. doi: 10.1016/0264-410X(91)90261-4. [DOI] [PubMed] [Google Scholar]
  • 3.Pollard E. The action of ionizing radiation on viruses. Advan Virus Res. 1955;2:109–151. doi: 10.1016/S0065-3527(08)60531-X. [DOI] [PubMed] [Google Scholar]
  • 4.Dertinger H., Jung H. Molecular Radiation Biology. NY: Springer-Verlag; 1970. The action of radiation on viruses; pp. 174–194. [Google Scholar]
  • 5.Lombardo J.H., Smolko E.E. Biotechnological project with a gamma radiation source of 100,000 Ci. Radiat Phys Chem. 1990;35(4–6):585–589. [Google Scholar]
  • 6.Smolko E.E., Lombardo J.H. Virus inactivation studies using ion beams, electron and gamma irradiation. Nuclear Instrument and Method in physics research B. 2005;236:249–253. doi: 10.1016/j.nimb.2005.04.055. [DOI] [Google Scholar]
  • 7.Donn A., Castagnaro M., Donaldson A.I. Ultra structural and replicative of foot and mouth disease virus in persistently infected BHK-21 cells. Arch virol. 1995;140:13–25. doi: 10.1007/BF01309720. [DOI] [PubMed] [Google Scholar]
  • 8.Reed L.J., Muench H. A simple method of estimating 50% end points. Am J Hyg. 1938;27:493–497. [Google Scholar]
  • 9.FRESCURA T., VIVOLI P. Studies of the FMD virus sub-types using Antigens Inactivated by gamma radiations. Zbl Vet Med B. 1973;20:822–825. doi: 10.1111/j.1439-0450.1973.tb02055.x. [DOI] [PubMed] [Google Scholar]
  • 10.Kolmer J.A. Serum diagnosis by complement fixation test. Philadelphia: Lea and Febiger Publishers; 1928. [Google Scholar]
  • 11.Balamurugan V., Renji R., Saha S. N., Reddy G. R., Gopalakishna S. Protective immune response of the capsid precursor polypeptide of FMDV type O produced in Pichia Pastoris. Virus Rese. 2003;9:141–149. doi: 10.1016/S0168-1702(02)00357-X. [DOI] [PubMed] [Google Scholar]
  • 12.Berinstein A., Tami C., Taboga O., Smitsaart E., Carrillo E. Protective immunity against foot and mouth disease virus induced by a recombinant vaccinia virus. Vaccine. 2000;18:2231–2238. doi: 10.1016/S0264-410X(99)00561-7. [DOI] [PubMed] [Google Scholar]
  • 13.Karber (2002) FMD, Kaber Formula for calculation of virus/antibody titres. OIEA Manual, Overview
  • 14.Leucam F., Fedidia M., Dannacher G. Measurement of post vaccinal immunity to foot and mouth disease in cattle by test on guinea pigs. Rev Med Vet. 1964;115:225–245. [Google Scholar]
  • 15.Pinto A. A., Garland A. J. M. Immune response to virus infection associated antigen in cattle repeated vaccinated with foot and mouth disease virus inactivated by formalin or acetylethyleneimine. J Hyg Camb. 1979;82:41–51. doi: 10.1017/s0022172400025456. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Indian Journal of Microbiology are provided here courtesy of Springer

RESOURCES