Abstract
The hexane, ethyl acetate, dichloromethane, methanol extracts and spent media (extracellular substances) were tested in vitro for their antibacterial activity for which one Gram-positive bacterium (Staphylococcus aureus) and four Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and Klebsiella pneumoniae) were used as test organisms. The methanol extract showed more potent activity than other organic extracts, spent medium of the culture exhibited little activity against E. coli only. No inhibitory effect was found against Klebsiella pneumoniae.The broth microdilution assay gave minimum inhibitory concentrations (MIC) values ranging from 1 to 512 μg/ml. The MIC of methanol extract against S. aureus and E. coli were 128 μg/ml and 256 μg/ml, respectively.
Keywords: Antibacterial activity, Spirulina platensis, Minimum inhibitory concentration
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References
- 1.Kreitlow S., Mundt S., Lindequist U. Cyanobacteria — a potential source of new biologically active substances. Journal of Biotechnology. 1999;70:61–63. doi: 10.1016/S0168-1656(99)00058-9. [DOI] [PubMed] [Google Scholar]
- 2.Falch B.S., Konig G.M., Wright A.D., Sticher O., Angerhofer C.K., Pezzuto J.M., Bachmann H. Biological activities of cyanobacteria:Evaluation of extracts and pure compounds. Planta Med. 1995;61:321–328. doi: 10.1055/s-2006-958092. [DOI] [PubMed] [Google Scholar]
- 3.Mundt S., kreitlow S., Nowotny A., Effmert U. Biological and pharmacological investigation of selected cyanobacteria. Int J Hyg Environ Health. 2001;203:327–334. doi: 10.1078/1438-4639-00045. [DOI] [PubMed] [Google Scholar]
- 4.MacMillan J.B., Ernst-Russell M.A., Ropp J.S., Molinski T.F. Lobocyclamides A-C, lipopeptides from a cryptic cyanobacterium mat containing Lyngbya confervoides. J Org Chem. 2002;67:8210–8215. doi: 10.1021/jo0261909. [DOI] [PubMed] [Google Scholar]
- 5.Luesch H., Yoshida W.Y., Moore R.E., Paul V.J., Mooberry S.L. Isolation, Structure determination and biological activity of lyngbyabellin A from the marine cyanobacterium Lyngbya majuscula. J Nat Prod. 2000;63:611–615. doi: 10.1021/np990543q. [DOI] [PubMed] [Google Scholar]
- 6.Papke U., Gross E.M., Francke W. Isolation, identification and determination of the absolute configuration of fischerellin B. A new algicide from the fresh water cyan obacterium Fischerella muscicola (Thuret) Tetrahedron Lett. 1997;38:379–382. doi: 10.1016/S0040-4039(96)02284-8. [DOI] [Google Scholar]
- 7.Koehn F.E., Longley R.E., Reed J.K. Microcolins A and B, new immunosuppressive peptide from the blue-green alga Lyngbya majuscula. J Nat Prod. 1992;55:613–619. doi: 10.1021/np50083a009. [DOI] [PubMed] [Google Scholar]
- 8.Hayashi T., Hayashi K. Calcium spirulan, an inhibitor of enveloped virus replication, from a blue-green alga Spirulina platensis. J Nat Prod. 1996;59:83–87. doi: 10.1021/np960017o. [DOI] [PubMed] [Google Scholar]
- 9.Perez C., Pauli M., Bazerque P. An antibiotic assay by agar-well diffusion method. Acta Biologiae et Medecine Experimentaalis. 1990;15:113–115. [Google Scholar]
- 10.NCCLS-National Committee For Clinical Laboratory Standards. (1997a) Methods For Dilution Antimicrobial Susceptibility tests for Bacteria that Grow Aerobically, Approved Standard M7-A4, Wayne, Pa
- 11.Asthana R.K., Srivastava A., Singh A.P., Deepali, Singh S.P., Nath G., Srivastava R., Srivastava B.S. Identification of an antimicrobial entity from the cyanobacterium Fischerella sp. isolated from bark of Azadirachta indica (Neem) tree. Journal of Applied Phycology. 2006;18(1):33–39. doi: 10.1007/s10811-005-9011-9. [DOI] [Google Scholar]
- 12.Issa A.A. Antibiotic production by the cyanobacteria Oscillatoria angustissima and Calothrix parietina. Env Toxic Pharm. 1999;8:33–37. doi: 10.1016/S1382-6689(99)00027-7. [DOI] [PubMed] [Google Scholar]
- 13.Bloor S., England R.R. Antibiotic production by the cyanobacterium Nostoc muscorum. Journal of Applied Phycology. 1989;1:367–372. doi: 10.1007/BF00003474. [DOI] [Google Scholar]
- 14.Fish S.A., Codd G.A. Bioactive compound production by thermophilic and thermotolerant cyanobacteria (blue-green algae) World J Microbial Biotech. 1994;10:338–341. doi: 10.1007/BF00414875. [DOI] [PubMed] [Google Scholar]
- 15.Ghasemi Y., Yazdi M.T., Shafiee A., Amini M., Shokravi S., Zarrini G. Parsiguine, A Novel Antimicrobial substance from Fischerella ambigua. Pharmceutical Biology. 2004;42(4–5):318–322. doi: 10.1080/13880200490511918. [DOI] [Google Scholar]