Abstract
Various parts of Datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against Gram-negative bacteria (Escherichia coli and Salmonella typhi) and Gram-positive bacteria (Bacillus cereus, Bacillus subtilis and Staphylococcus aureus). The results of agar well diffusion assay indicated that the pattern of inhibition depends largely upon the plant part, solvent used for extraction and the organism tested. Extracts prepared from leaves were shown to have better efficacy than stem and root extracts. Organic extracts provided potent antibacterial activity as compared to aqueous extracts. Among all the extracts, methanolic extract was found most active against almost all the bacterial species tested. Gram-positive bacteria were found most sensitive as compared to Gram-negative bacteria. Staphylococcus aureus was signifi cantly inhibited by almost all the extracts even at very low MIC followed by other Gram-positives. For Escherichia coli (a Gram-negative bacterium), the end point was not reached for ethyl acetate extract while it was very high for other extracts. The study promises an interesting future for designing a potentially active antibacterial agent from Datura innoxia.
Keywords: Datura innoxia, Antibacterial Activity, Drug Resistance, Agar Well Diffusion Assay, Minimum Inhibitory Concentration
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References
- 1.Cohen M.L. Epidemiology of drug resistance, implications for a post antimicrobial era. Science. 1992;257:1050–1055. doi: 10.1126/science.257.5073.1050. [DOI] [PubMed] [Google Scholar]
- 2.Gold S.G., Moellering R.C. Antimicrobial drug resistance. N Engl J Med. 1996;335:1445–1453. doi: 10.1056/NEJM199611073351907. [DOI] [PubMed] [Google Scholar]
- 3.Kaushik P. Glimpses of medical botany in Atharvaveda. The Vedic Path. 1985;48(2):64–67. [Google Scholar]
- 4.Nostro A., Germano M.P., Angelo V.D., Marino A., Cannatelli M.A. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Letters in Applied Microbiology. 2000;30:379–384. doi: 10.1046/j.1472-765x.2000.00731.x. [DOI] [PubMed] [Google Scholar]
- 5.Kaushik P and Dhiman AK (2000) Medicinal Plants and Raw Drugs of India. Bishen Singh Mahendra Pal Singh, New Cannaught Place, Dehradun
- 6.Samie A., Obi C.L., Bessong P.O., Namrita L. Activity profiles of fourteen selected medicinal plants from rural Venda communities in South Africa against fifteen clinical bacterial species. African Journal of Biotechnology. 2005;4(12):1443–1451. [Google Scholar]
- 7.Kaushik P. Indigenous Medicinal Plants including Microbes and Fungi. New Delhi: Today and Tomorrow’s Printers & Publishers; 1988. [Google Scholar]
- 8.Figueiredo S.F.L., Esquibel M.A. Callogenesis, Organogenesis and Micropropagation of Datura insignis. Barb Rodr R Bras Fisiol Veg. 1991;3(2):63–98. [Google Scholar]
- 9.NCCLS-National Committee for Clinical Laboratory Standards (1997b) Methods for Determining Bactericidal Activity of Antimicrobial Agents, Approved Standards M26-T, Wayne, Pa
- 10.NCCLS-National Committee for Clinical Laboratory Standards (1997a) Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standards M7-A4, Wayne, Pa
- 11.Akinpelu D.A., Onakoya T.M. Antimicrobial activities of medicinal plants used in folklore remedies in south-western. African Journal of Biotechnology. 2006;5(11):1078–1081. [Google Scholar]
- 12.Dulger B. Antimicrobial activity of some endemic Scrophulariaceae from Turkey. Pharmaceutical Biology. 2006;44(9):672–676. doi: 10.1080/13880200601009099. [DOI] [Google Scholar]
- 13.Dulger B., Kirmizi S., Arslan H., Guleryuz G. Antimicrobial activity of three endemic Verbascum species. Pharmaceutical Biology. 2002;40(8):587–589. doi: 10.1076/phbi.40.8.587.14657. [DOI] [Google Scholar]
- 14.Aliero A.A., Afolayan A.J. Antimicrobial activity of Solanum tomentosum. African Journal of Biotechnology. 2006;5(4):369–372. [Google Scholar]
- 15.Kaushik P., Singh Y. Antibacterial activity of extract of rhizome of Curcuma longa (turmeric) J Indian Bot Soc. 2000;79:191–192. [Google Scholar]
- 16.Thongson C., Davidson P.M., Mahakarnchanakul W., Weiss J. Antimicrobial activity of ultrasound-assisted solvent-extracted spices. Letters in Applied Microbiology. 2004;39:401–406. doi: 10.1111/j.1472-765X.2004.01605.x. [DOI] [PubMed] [Google Scholar]
- 17.Gnan S.O., Demello M.T. Inhibition of Staphylococcus aureus by aqueous Goiaba extracts. Journal of Ethnopharmacology. 1999;68(1–3):103–108. doi: 10.1016/S0378-8741(99)00048-3. [DOI] [PubMed] [Google Scholar]
- 18.Eftekhar F., Yousefzadi M., Tafakori V. Antimicrobial activity of Datura innoxia and Datura stramonium. Fitoterapia. 2005;76:118–120. doi: 10.1016/j.fitote.2004.10.004. [DOI] [PubMed] [Google Scholar]