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. 1982 Sep;37(3):1295–1298. doi: 10.1128/iai.37.3.1295-1298.1982

High-Molecular-Weight Plasmid Correlates with Escherichia coli Enteroinvasiveness

Jeffrey R Harris 1, I Kaye Wachsmuth 1, Betty R Davis 1, Mitchell L Cohen 1
PMCID: PMC347680  PMID: 6752026

Abstract

We tested by the Sereny method and examined for plasmids 14 previously Sereny-test-positive and 17 previously untested Escherichia coli strains. The presence of a 140-megadalton plasmid was significantly associated with enteroin-vasiveness as measured by the Sereny test in the 31 E. coli strains examined.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Formal S. B., Baron L. S., Kopecko D. J., Washington O., Powell C., Life C. A. Construction of a potential bivalent vaccine strain: introduction of Shigella sonnei form I antigen genes into the galE Salmonella typhi Ty21a typhoid vaccine strain. Infect Immun. 1981 Dec;34(3):746–750. doi: 10.1128/iai.34.3.746-750.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Guerry P., LeBlanc D. J., Falkow S. General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973 Nov;116(2):1064–1066. doi: 10.1128/jb.116.2.1064-1066.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Humphreys G. O., Willshaw G. A., Anderson E. S. A simple method for the preparation of large quantities of pure plasmid DNA. Biochim Biophys Acta. 1975 Apr 2;383(4):457–463. doi: 10.1016/0005-2787(75)90318-4. [DOI] [PubMed] [Google Scholar]
  6. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moseley S. L., Huq I., Alim A. R., So M., Samadpour-Motalebi M., Falkow S. Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. J Infect Dis. 1980 Dec;142(6):892–898. doi: 10.1093/infdis/142.6.892. [DOI] [PubMed] [Google Scholar]
  8. Orskov F. Virulence factors of the bacterial cell surface. J Infect Dis. 1978 May;137(5):630–633. doi: 10.1093/infdis/137.5.630. [DOI] [PubMed] [Google Scholar]
  9. SERENY B. Experimental shigella keratoconjunctivitis; a preliminary report. Acta Microbiol Acad Sci Hung. 1955;2(3):293–296. [PubMed] [Google Scholar]
  10. Sansonetti P. J., Kopecko D. J., Formal S. B. Involvement of a plasmid in the invasive ability of Shigella flexneri. Infect Immun. 1982 Mar;35(3):852–860. doi: 10.1128/iai.35.3.852-860.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Sansonetti P. J., Kopecko D. J., Formal S. B. Shigella sonnei plasmids: evidence that a large plasmid is necessary for virulence. Infect Immun. 1981 Oct;34(1):75–83. doi: 10.1128/iai.34.1.75-83.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Silva R. M., Toledo M. R., Trabulsi L. R. Biochemical and cultural characteristics of invasive Escherichia coli. J Clin Microbiol. 1980 May;11(5):441–444. doi: 10.1128/jcm.11.5.441-444.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Toledo M. R., Reis M. H., Almeida R. G., Trabulsi L. R. Invasive strain of Escherichia coli belonging to O group 29. J Clin Microbiol. 1979 Feb;9(2):288–289. doi: 10.1128/jcm.9.2.288-289.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Tulloch E. F., Jr, Ryan K. J., Formal S. B., Franklin F. A. Invasive enteropathic Escherichia coli dysentery. An outbreak in 28 adults. Ann Intern Med. 1973 Jul;79(1):13–17. doi: 10.7326/0003-4819-79-1-13. [DOI] [PubMed] [Google Scholar]

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