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Infection and Immunity logoLink to Infection and Immunity
. 1982 Oct;38(1):127–135. doi: 10.1128/iai.38.1.127-135.1982

Measurement of bacterial cell wall in tissues by solid-phase radioimmunoassay: correlation of distribution and persistence with experimental arthritis in rats.

R Eisenberg, A Fox, J J Greenblatt, S K Anderle, W J Cromartie, J H Schwab
PMCID: PMC347707  PMID: 6754610

Abstract

We have developed sensitive and specific solid-phase radioimmunoassays to quantitate the distribution and persistence of bacterial antigen in rats developing arthritis in response to a single injection of streptococcal cell wall material. Three separate assays were specific for either the A polysaccharide (N-acetyl-D-glucosamine), A-variant polysaccharide (polyrhamnose), or peptidoglycan (D-ala-D-ala) moieties of the streptococcal cell wall. Antigen was detected in all tissues surveyed, although the greatest amount was in the liver and spleen. By using three fractions of cell wall separated by size, we have shown that the development of arthritis correlates with the degree of cell wall deposited and persisting in the joints. Further statistical analyses suggested differences in metabolism by different tissues and differential metabolism of different antigenic epitopes in some cases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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