Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1982 Oct;38(1):183–188. doi: 10.1128/iai.38.1.183-188.1982

Transduction of the genetic determinant for streptolysin S in group A streptococci.

S A Skjold, W R Maxted, L W Wannamaker
PMCID: PMC347716  PMID: 6754613

Abstract

The genetic determinant for streptolysin S production (SLS+) was successfully transduced to two naturally occurring nonhemolytic strains of group A streptococci (Streptococcus pyogenes), an M-type 18 strain associated with an outbreak of rheumatic fever and an M-negative variant of a type 49 strain isolated from a skin lesion. Attempts to transduce this determinant to a nonhemolytic M-type 68 strain and a nonhemolytic T-type 12 strain were not successful. Transduction was accomplished with a double temperature-sensitive mutant bacteriophage. Cellular antigenic characters and the phage sensitivity of the transductants remained unaltered. The donor strain also transduced streptomycin resistance well when the nonhemolytic type 49 strain was used as a recipient. There was no evidence of cotransduction of the determinants for streptolysin S and streptomycin resistance.

Full text

PDF
183

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball L. C. The influence of neopeptone on the formation of M-antigen by group A streptococci in culture. Med Lab Technol. 1972 Jan;29(1):18–26. [PubMed] [Google Scholar]
  2. FELLER A. E., STEVENS D. A. Sheep blood agar for the isolation of Lancefield groups of beta-hemolytic streptococci. J Lab Clin Med. 1952 Mar;39(3):484–491. [PubMed] [Google Scholar]
  3. James L., McFarland R. B. An epidemic of pharyngitis due to a nonhemolytic group A streptococcus at lowry air force base. N Engl J Med. 1971 Apr 8;284(14):750–752. doi: 10.1056/NEJM197104082841403. [DOI] [PubMed] [Google Scholar]
  4. Malke H., Köhler W. Transduction among group A Streptococci: transducibility of strains representative of thirty different M types. Zentralbl Bakteriol Orig A. 1973 Jul;224(2):194–201. [PubMed] [Google Scholar]
  5. Malke H. Transduction of Streptococcus pyogenes K 56 by temperature-sensitive mutants of the transducing phage A 25. Z Naturforsch B. 1969 Dec;24(12):1556–1561. doi: 10.1515/znb-1969-1214. [DOI] [PubMed] [Google Scholar]
  6. Oliver D. R., Brown B. L., Clewell D. B. Characterization of plasmids determining hemolysin and bacteriocin production in Streptococcus faecalis 5952. J Bacteriol. 1977 May;130(2):948–950. doi: 10.1128/jb.130.2.948-950.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Owens W., Henley F., Barridge B. D. Hemolytic mutants of group A Streptococcus pyogenes. J Clin Microbiol. 1978 Feb;7(2):153–157. doi: 10.1128/jcm.7.2.153-157.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Pinney A. M., Widdowson J. P., Maxted W. R. Inhibition of beta-haemolysis by opacity factor in group A streptococci. J Hyg (Lond) 1977 Jun;78(3):355–362. doi: 10.1017/s0022172400056254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Skjold S. A., Wannamaker L. W. Method for phage typing group A type 49 streptococci. J Clin Microbiol. 1976 Sep;4(3):232–238. doi: 10.1128/jcm.4.3.232-238.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Tagg J. R., Skjold S., Wannamaker L. W. Transduction of bacteriocin determinants in group A streptococci. J Exp Med. 1976 Jun 1;143(6):1540–1544. doi: 10.1084/jem.143.6.1540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. WILLIAMS R. E. Laboratory diagnosis of streptococcal infections. Bull World Health Organ. 1958;19(1):153–176. [PMC free article] [PubMed] [Google Scholar]
  12. Wannamaker L. W., Almquist S., Skjold S. Intergroup phage reactions and transduction between group C and group A streptococci. J Exp Med. 1973 Jun 1;137(6):1338–1353. doi: 10.1084/jem.137.6.1338. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Widdowson J. P., Maxted W. R., Grant D. L. The production of opacity in serum by group A streptococci and its relationship withthe presence of M antigen. J Gen Microbiol. 1970 Jun;61(3):343–353. doi: 10.1099/00221287-61-3-343. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES