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. 1982 Oct;38(1):375–379. doi: 10.1128/iai.38.1.375-379.1982

Mitogenic activity in human embryonic fibroblasts early after infection by human cytomegalovirus.

N Takehara, K Ryoke, T Kurimura
PMCID: PMC347741  PMID: 6292097

Abstract

We have characterized the nonspecific lymphocyte stimulation by extracts of human cytomegalovirus-infected human embryonic fibroblasts. Cell extracts prepared at 5 h postinfection (early extract) and 72 h postinfection (late extract) were both highly mitogenic in lymphocyte preparations from adult blood, cord blood, and rabbit blood. Maximum stimulation of the lymphocytes was observed on day 3 after the addition of early or late extract under optimal conditions. Early extract stimulated both the E-rosetting and the EAC-rosetting subpopulations of human lymphocytes. The mitogenic activity appeared before 5 h postinfection and was fairly stable at 30 degrees C for 5 h.

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Selected References

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