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. 1982 Nov;38(2):455–461. doi: 10.1128/iai.38.2.455-461.1982

Neutralizing antibody response of rabbits and goats to caprine arthritis-encephalitis virus.

P Klevjer-Anderson, T C McGuire
PMCID: PMC347761  PMID: 6292102

Abstract

Rabbits were immunized with purified caprine arthritis-encephalitis virus and examined for neutralizing activity. Analysis of virus-antiserum interaction at 37 degrees C demonstrated little loss of viral infectivity after incubation with heat-inactivated rabbit antiserum for 60 min. However, sensitization of virus (as assessed by the addition of complement) occurred almost immediately and was 95% complete after 10 min. The complement-dependent neutralizing activity was associated with the immunoglobulin G fraction of rabbit antiserum. Addition of goat anti-rabbit immunoglobulin G to the immune rabbit serum-caprine arthritis-encephalitis virus mixture also resulted in neutralization of infectivity when unbound antibody was removed before addition of the anti-immunoglobulin. Serum from most caprine arthritis-encephalitis virus-infected goats contains antibody activity to the core protein p28, as demonstrated by immunodiffusion and enzyme-linked immunosorbent assay. However, attempts to demonstrate neutralizing activity in the serum of goats up to 1.5 years post-inoculation or in serum of hyperimmunized goats were unsuccessful when the sera were examined alone or in combination with complement or rabbit anti-goat immunoglobulin or both.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams D. S., Crawford T. B., Banks K. L., McGuire T. C., Perryman L. E. Immune responses of goats persistently infected with caprine arthritis-encephalitis virus. Infect Immun. 1980 May;28(2):421–427. doi: 10.1128/iai.28.2.421-427.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adams D. S., Crawford T. B., Klevjer-Anderson P. A pathogenetic study of the early connective tissue lesions of viral caprine arthritis-encephalitis. Am J Pathol. 1980 May;99(2):257–278. [PMC free article] [PubMed] [Google Scholar]
  3. Bartholomew R. M., Esser A. F., Müller-Eberhard H. J. Lysis of oncornaviruses by human serum. Isolation of the viral complement (C1) receptor and identification as p15E. J Exp Med. 1978 Mar 1;147(3):844–853. doi: 10.1084/jem.147.3.844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cheevers W. P., Roberson S., Klevjer-Anderson P., Crawford T. B. Characterization of caprine arthritis-encephalitis virus: a retrovirus of goats. Arch Virol. 1981;67(1):111–117. doi: 10.1007/BF01314610. [DOI] [PubMed] [Google Scholar]
  5. Coggins L. African swine fever virus. Pathogenesis. Prog Med Virol. 1974;18(0):48–63. [PubMed] [Google Scholar]
  6. Cork L. C., Hadlow W. J., Crawford T. B., Gorham J. R., Piper R. C. Infectious leukoencephalomyelitis of young goats. J Infect Dis. 1974 Feb;129(2):134–141. doi: 10.1093/infdis/129.2.134. [DOI] [PubMed] [Google Scholar]
  7. Crawford T. B., Adams D. S., Cheevers W. P., Cork L. C. Chronic arthritis in goats caused by a retrovirus. Science. 1980 Feb 29;207(4434):997–999. doi: 10.1126/science.6153243. [DOI] [PubMed] [Google Scholar]
  8. Crawford T. B., Adams D. S., Sande R. D., Gorham J. R., Henson J. B. The connective tissue component of the caprine arthritis-encephalitis syndrome. Am J Pathol. 1980 Aug;100(2):443–454. [PMC free article] [PubMed] [Google Scholar]
  9. Gardner M. B. Type C viruses of wild mice: characterization and natural history of amphotropic, ecotropic, and xenotropic MuLv. Curr Top Microbiol Immunol. 1978;79:215–259. doi: 10.1007/978-3-642-66853-1_5. [DOI] [PubMed] [Google Scholar]
  10. Kane J. P., Hardman D. A., Dimpfl J. C., Levy J. A. Apolipoprotein is responsible for neutralization of xenotropic type C virus by mouse serum. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5957–5961. doi: 10.1073/pnas.76.11.5957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Klevjer-Anderson P., Anderson L. W. Caprine arthritis-encephalitis virus infection of caprine monocytes. J Gen Virol. 1982 Jan;58(Pt 1):195–198. doi: 10.1099/0022-1317-58-1-195. [DOI] [PubMed] [Google Scholar]
  12. Klevjer-Anderson P., Cheevers W. P. Characterization of the infection of caprine synovial membrane cells by the retrovirus caprine arthritis-encephalitis virus. Virology. 1981 Apr 15;110(1):113–119. doi: 10.1016/0042-6822(81)90012-x. [DOI] [PubMed] [Google Scholar]
  13. Kono Y., Kobayashi K., Fukunaga Y. Antigenic drift of equine infectious anemia virus in chronically infected horses. Arch Gesamte Virusforsch. 1973;41(1):1–10. doi: 10.1007/BF01249923. [DOI] [PubMed] [Google Scholar]
  14. Majer M. Virus sensitization. Curr Top Microbiol Immunol. 1972;58:69–84. doi: 10.1007/978-3-642-65357-5_2. [DOI] [PubMed] [Google Scholar]
  15. Massey R. J., Schochetman G. Viral epitopes and monoclonal antibodies: isolation of blocking antibodies that inhibit virus neutralization. Science. 1981 Jul 24;213(4506):447–449. doi: 10.1126/science.6264601. [DOI] [PubMed] [Google Scholar]
  16. McGuire T. C., Musoke A. J., Kurtti T. Functional properties of bovine IgG1 and IgG2: interaction with complement, macrophages, neutrophils and skin. Immunology. 1979 Oct;38(2):249–256. [PMC free article] [PubMed] [Google Scholar]
  17. Narayan O., Griffin D. E., Clements J. E. Virus mutation during 'slow infection': temporal development and characterization of mutants of visna virus recovered from sheep. J Gen Virol. 1978 Nov;41(2):343–352. doi: 10.1099/0022-1317-41-2-343. [DOI] [PubMed] [Google Scholar]
  18. Oroszlan S., Nowinski R. C. Lysis of retroviruses with monoclonal antibodies against viral envelope proteins. Virology. 1980 Feb;101(1):296–299. doi: 10.1016/0042-6822(80)90507-3. [DOI] [PubMed] [Google Scholar]
  19. Schochetman G., Arthur L. O., Long C. W., Massey R. J. Mice with spontaneous mammary tumors develop type-specific neutralizing and cytotoxic antibodies against the mouse mammary tumor virus envelope protein gp52. J Virol. 1979 Oct;32(1):131–139. doi: 10.1128/jvi.32.1.131-139.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Snyder D. B., Myrup A. C., Dutta S. K. Complement requirement for virus neutralization by antibody and reduced serum complement levels associated with experimental equine herpesvirus 1 infection. Infect Immun. 1981 Feb;31(2):636–640. doi: 10.1128/iai.31.2.636-640.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Watarai S., Onuma M., Mikami T., Izawa H. Enhancement of antibody activity to bovine leukemia virus by complement. Arch Virol. 1981;68(2):135–142. doi: 10.1007/BF01314443. [DOI] [PubMed] [Google Scholar]

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