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. 1982 Nov;38(2):588–591. doi: 10.1128/iai.38.2.588-591.1982

In vitro effect of murine-derived transfer factor on Salmonella-specific rosette formation.

R A Smith, A Esa
PMCID: PMC347779  PMID: 6183216

Abstract

The splenic lymphocytes from Salmonella-immune ICR Swiss or C3H/HeJ mice formed greater than 0.2% antigen-specific rosettes with sheep erythrocytes coated with a spent-medium protein antigen of Salmonella typhimurium. These rosette-forming lymphocytes were found to be sensitive to the effects of antithymocyte serum plus complement. Transfer factor prepared from the Salmonella-immune splenic lymphocytes of ICR Swiss mice was active in sensitizing nonimmune ICR Swiss or C3H/HeJ lymphocytes to form greater than or equal to 0.2% rosettes with salmonella antigen-coated sheep erythrocytes. These rosettes were also sensitive to antithymocyte serum and complement. Few rosettes were formed between the transfer factor-treated lymphocytes and sheep erythrocytes coated with a Listeria protein antigen. A nonimmune dialysate preparation was inactive in sensitizing nonimmune lymphocytes, as indicated by a lack of rosette formation. Neither the immune transfer factor nor the nonimmune dialysate had any enhancing or abrogating effect upon rosette formation by splenic lymphocytes from Salmonella-immune mice. The enumeration of antigen-specific rosettes may be a useful means of assaying for transfer factor activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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